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Questions and Answers
In a dihybrid cross, what phenotypic ratio is typically observed in the offspring, assuming independent assortment?
In a dihybrid cross, what phenotypic ratio is typically observed in the offspring, assuming independent assortment?
- 3:1
- 1:1:1:1
- 9:3:3:1 (correct)
- 1:2:1
What condition is essential for observing the classic 9:3:3:1 phenotypic ratio in a dihybrid cross?
What condition is essential for observing the classic 9:3:3:1 phenotypic ratio in a dihybrid cross?
- One of the genes must exhibit dominance, while the other exhibits incomplete dominance.
- The genes must be located close together on the same chromosome.
- The genes must exhibit complete linkage.
- The alleles at both genes must be assorting randomly with respect to each other. (correct)
What is the probability of producing a gamete with the genotype pt from an individual with the genotype PpTt, assuming independent assortment?
What is the probability of producing a gamete with the genotype pt from an individual with the genotype PpTt, assuming independent assortment?
- 1/2
- 1/4 (correct)
- 1/8
- 1/16
According to Mendel's principle of independent assortment, how do alleles of different genes behave during gamete formation?
According to Mendel's principle of independent assortment, how do alleles of different genes behave during gamete formation?
What is the expected frequency of each possible gamete combination from a dihybrid individual, assuming independent assortment?
What is the expected frequency of each possible gamete combination from a dihybrid individual, assuming independent assortment?
In a monohybrid cross (Pp x Pp), what is the probability of obtaining a homozygous recessive (pp) offspring?
In a monohybrid cross (Pp x Pp), what is the probability of obtaining a homozygous recessive (pp) offspring?
What does Mendel's Law of Segregation state?
What does Mendel's Law of Segregation state?
In the context of a Chi-Square test, what does it mean to 'reject the null hypothesis'?
In the context of a Chi-Square test, what does it mean to 'reject the null hypothesis'?
In a monohybrid cross where both parents are heterozygous (Pp), what is the expected phenotypic ratio of the offspring?
In a monohybrid cross where both parents are heterozygous (Pp), what is the expected phenotypic ratio of the offspring?
A researcher performs a cross and observes a phenotypic ratio of 2.8:1. If the expected ratio is 3:1, what statistical test can be used to determine if the observed results are significantly different from the expected results?
A researcher performs a cross and observes a phenotypic ratio of 2.8:1. If the expected ratio is 3:1, what statistical test can be used to determine if the observed results are significantly different from the expected results?
If the Chi-Square test statistic ($χ^2$) is less than the critical value, what conclusion can be drawn?
If the Chi-Square test statistic ($χ^2$) is less than the critical value, what conclusion can be drawn?
In the context of genetic crosses, what is the significance of generating F1 offspring?
In the context of genetic crosses, what is the significance of generating F1 offspring?
A plant breeder crosses two pea plants and obtains the following results: 600 purple-flowered plants and 200 white-flowered plants. Based on this data, what is the approximate observed phenotypic ratio?
A plant breeder crosses two pea plants and obtains the following results: 600 purple-flowered plants and 200 white-flowered plants. Based on this data, what is the approximate observed phenotypic ratio?
In Mendel's dihybrid cross experiment, what phenotypic ratio is expected in the offspring if the genes assort independently?
In Mendel's dihybrid cross experiment, what phenotypic ratio is expected in the offspring if the genes assort independently?
What is the purpose of calculating a chi-square ($\chi^2$) value in the context of Mendel's experiments?
What is the purpose of calculating a chi-square ($\chi^2$) value in the context of Mendel's experiments?
In Mendel's dihybrid cross data, if the calculated $\chi^2$ value is 4.13 with 3 degrees of freedom, and the corresponding p-value is 0.25, what conclusion can be drawn?
In Mendel's dihybrid cross data, if the calculated $\chi^2$ value is 4.13 with 3 degrees of freedom, and the corresponding p-value is 0.25, what conclusion can be drawn?
Mendel's laws of inheritance directly refuted which prevailing idea about heredity?
Mendel's laws of inheritance directly refuted which prevailing idea about heredity?
In a dihybrid cross involving two traits, if the observed numbers of offspring phenotypes are purple/tall (372), purple/short (113), white/tall (126), and white/short (29) out of a total of 640, which calculation is used to determine the expected number of purple/tall offspring, assuming independent assortment?
In a dihybrid cross involving two traits, if the observed numbers of offspring phenotypes are purple/tall (372), purple/short (113), white/tall (126), and white/short (29) out of a total of 640, which calculation is used to determine the expected number of purple/tall offspring, assuming independent assortment?
Which process directly leads to the segregation of alleles, as described by Mendel's Law of Segregation?
Which process directly leads to the segregation of alleles, as described by Mendel's Law of Segregation?
In a monohybrid cross, if the F1 generation consistently displays the trait of only one parent, which principle is being demonstrated?
In a monohybrid cross, if the F1 generation consistently displays the trait of only one parent, which principle is being demonstrated?
A plant has the genotype AaBb for two independently assorting genes. What proportion of its gametes will be Ab, assuming independent assortment?
A plant has the genotype AaBb for two independently assorting genes. What proportion of its gametes will be Ab, assuming independent assortment?
What is the expected outcome of a reciprocal cross?
What is the expected outcome of a reciprocal cross?
In the context of quantitative traits, which equation best represents the relationship between phenotype, genotype, and environment?
In the context of quantitative traits, which equation best represents the relationship between phenotype, genotype, and environment?
Mendel chose pea plants for his experiments for several reasons. Which of the following is NOT one of the reasons?
Mendel chose pea plants for his experiments for several reasons. Which of the following is NOT one of the reasons?
If a plant with the genotype 'Aa' self-pollinates, and 'A' is dominant for purple flowers while 'a' is recessive for white flowers, what proportion of the F1 generation would you expect to have white flowers?
If a plant with the genotype 'Aa' self-pollinates, and 'A' is dominant for purple flowers while 'a' is recessive for white flowers, what proportion of the F1 generation would you expect to have white flowers?
What is the most accurate definition of an allele?
What is the most accurate definition of an allele?
In Mendelian genetics, what distinguishes alleles from genes?
In Mendelian genetics, what distinguishes alleles from genes?
What is the primary difference between the F1 and F2 generations in Mendelian genetics?
What is the primary difference between the F1 and F2 generations in Mendelian genetics?
Which of the following best describes Mendel's approach to studying inheritance?
Which of the following best describes Mendel's approach to studying inheritance?
If 'D' represents the dominant allele for tall plants and 'd' represents the recessive allele for dwarf plants, what genotypes would be considered homozygous?
If 'D' represents the dominant allele for tall plants and 'd' represents the recessive allele for dwarf plants, what genotypes would be considered homozygous?
Suppose a researcher is studying a Mendelian trait in pea plants and observes a 3:1 phenotypic ratio in the F2 generation. What can they infer about the genotypes of the F1 generation?
Suppose a researcher is studying a Mendelian trait in pea plants and observes a 3:1 phenotypic ratio in the F2 generation. What can they infer about the genotypes of the F1 generation?
If a true-breeding tall plant (TT) is crossed with a true-breeding dwarf plant (tt), what will be the genotype of the F1 generation?
If a true-breeding tall plant (TT) is crossed with a true-breeding dwarf plant (tt), what will be the genotype of the F1 generation?
A pea plant with round seeds (RR) is crossed with a pea plant with wrinkled seeds (rr). All the F1 generation plants have round seeds. If two plants from the F1 generation are crossed, what is the probability of obtaining a plant with wrinkled seeds in the F2 generation?
A pea plant with round seeds (RR) is crossed with a pea plant with wrinkled seeds (rr). All the F1 generation plants have round seeds. If two plants from the F1 generation are crossed, what is the probability of obtaining a plant with wrinkled seeds in the F2 generation?
In the context of Mendelian genetics, what does the reappearance of a trait in the F2 generation that was absent in the F1 generation indicate?
In the context of Mendelian genetics, what does the reappearance of a trait in the F2 generation that was absent in the F1 generation indicate?
In a chi-square test, what does a high P-value (e.g., 0.54) indicate regarding the null hypothesis?
In a chi-square test, what does a high P-value (e.g., 0.54) indicate regarding the null hypothesis?
How is the number of degrees of freedom (df) calculated in a chi-square test?
How is the number of degrees of freedom (df) calculated in a chi-square test?
A researcher performs a cross between two pea plants with the genotype Pp. They observe 650 purple flowers and 270 white flowers. Using a chi-square test, they calculate a χ2 value of 2.5 and have 1 degree of freedom. Based on a critical χ2 value of 3.841 (p=0.05), what conclusion can they draw?
A researcher performs a cross between two pea plants with the genotype Pp. They observe 650 purple flowers and 270 white flowers. Using a chi-square test, they calculate a χ2 value of 2.5 and have 1 degree of freedom. Based on a critical χ2 value of 3.841 (p=0.05), what conclusion can they draw?
What does Mendel's Principle of Segregation state?
What does Mendel's Principle of Segregation state?
What is the purpose of performing reciprocal crosses in genetics?
What is the purpose of performing reciprocal crosses in genetics?
Mendel's work disproved which of the following pre-existing ideas about inheritance?
Mendel's work disproved which of the following pre-existing ideas about inheritance?
How many alleles are present in a gamete for a single trait?
How many alleles are present in a gamete for a single trait?
Mendel crossed true-breeding plants with different traits, and noted that the F1 generation resembled only one of the parents. What does this observation suggest?
Mendel crossed true-breeding plants with different traits, and noted that the F1 generation resembled only one of the parents. What does this observation suggest?
Flashcards
Principle of Segregation
Principle of Segregation
Alleles for a gene are separated during gamete formation and reunite during fertilization.
Principle of Independent Assortment
Principle of Independent Assortment
The alleles of different genes assort independently during gamete formation.
Dihybrid Cross
Dihybrid Cross
A genetic cross that examines the inheritance of two different traits simultaneously.
Gametes Frequency 1/4
Gametes Frequency 1/4
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9:3:3:1 Ratio
9:3:3:1 Ratio
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Law of Segregation
Law of Segregation
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Law of Independent Assortment
Law of Independent Assortment
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Gene
Gene
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Allele
Allele
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Genotype
Genotype
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Phenotype
Phenotype
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Mendelian Traits
Mendelian Traits
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Quantitative Traits
Quantitative Traits
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Punnett Square
Punnett Square
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Monohybrid Cross
Monohybrid Cross
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F1 Generation
F1 Generation
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F2 Generation
F2 Generation
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Phenotypic Ratio
Phenotypic Ratio
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Chi-Square Test
Chi-Square Test
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3:1 Ratio
3:1 Ratio
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Homozygous
Homozygous
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Heterozygous
Heterozygous
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Dominant vs. Recessive Alleles
Dominant vs. Recessive Alleles
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Mendel's Dihybrid Cross
Mendel's Dihybrid Cross
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Observations vs. Expectations
Observations vs. Expectations
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Chi-Square (χ2) Test
Chi-Square (χ2) Test
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Independent Assortment
Independent Assortment
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Null Hypothesis (H0)
Null Hypothesis (H0)
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Observed vs. Expected
Observed vs. Expected
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Chi-Square (χ2)
Chi-Square (χ2)
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Degrees of Freedom
Degrees of Freedom
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P-value
P-value
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Mendel's Principle of Segregation
Mendel's Principle of Segregation
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Mendel's Principle of Independent Assortment
Mendel's Principle of Independent Assortment
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Reciprocal Crosses
Reciprocal Crosses
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Study Notes
Mendel's Principles/Laws
- Mendel's principles, encompassing segregation and independent assortment, arise from meiosis I and II.
- Mendel lacked knowledge of meiosis; subsequent researchers, using microscopes, connected chromosome movement with the inheritance of traits.
Review of Terms
- Gene: The fundamental unit of heredity, located on DNA (except RNA viruses).
- Allele: A variant form of a gene.
- Genotype: An organism's genetic makeup.
- Phenotype: Observable characteristics of an organism.
- Phenotype = Genotype + Environment + G X E.
- For Mendelian traits, Phenotype = Genotype.
Why Peas?
- Peas are easy to grow and produce many seeds.
- They have short generation times.
- Peas readily self-fertilize and can be cross-fertilized.
- Peas exhibit many categorical traits (traits not influenced by the environment).
How to Cross a Pea Plant
- Flowers are perfect.
- Remove stamens to prevent self-pollination.
- Collect pollen from another flower.
- Cross-fertilize using the pollen to reproduce the plant.
- Observe offspring phenotypes.
Mendel's Experiments
- Mendel looked at seven pea plant traits.
- He isolated true-breeding strains.
- He studied each trait separately.
- He designed careful breeding experiments.
- He diligently recorded his results.
- He used large sample sizes.
- He followed traits across multiple generations.
Mendel's 1st Law: Principle of Segregation
- The two alleles (members of a gene pair) separate during gamete formation.
- Alleles are randomly distributed to offspring; each offspring receives one allele from each parent.
Mendel's Principle of Segregation (Terminology)
- P Generation: Parental generation.
- F₁ Generation: First filial generation.
- F₂ Generation: Second filial generation.
- F₃, F₄, F₅: Subsequent generations.
- Monohybrid Cross: A mating between true-breeding strains that differ in one trait.
Terminology Continued: Reciprocal Crosses
- Reciprocal crosses are when the parental traits are switched from the parent flower to the other.
- For example the pollen of a purple flower is used to fertilize a white flower, then the pollen of a white flower is used to fertilize a purple flower. This is to see if the trait difference is between the pollen or the seed.
Principle of Uniformity of F₁
- F₁ offspring from a monohybrid cross resemble one parent (and each other).
1 Trait Disappears in the F₁
- One trait disappears in F₁ individuals.
Traits Reappear in F₂
- The missing trait appears in the F₂ generation.
- Mendel correctly reasoned that the information to produce the trait was present in the F₁ generation in the form of "factors" (now called genes) and was not blended.
Letters used to Designate Alleles
- Dominant allele is represented using uppercase letters (e.g., P).
- Recessive allele is represented using lowercase letters (e.g., p).
Heterozygous vs. Homozygous
- Homozygotes have two copies of the same allele (e.g., PP or pp).
- Heterozygotes have one copy of each allele (e.g., Pp).
Same Flower Example
- This represents the self-pollination and the F₁ generation.
Punnett Square: Let's Make an F₁
- A Punnett square demonstrates a hypothetical crossing of a parental plant with PP and pp phenotypes (showing 100% of the offspring having a Pp phenotype).
Punnett Square: Monohybrid Cross
- A monohybrid cross is the mating of parental individuals that differ only in one character trait.
Punnett Square: Monohybrid Cross (Crossing F1 Individuals)
- A Punnett square for a monohybrid cross.
If Factors Segregate
- If factors segregate in F₁, then the expected phenotypic ratio in offspring is 3:1.
How Did Mendel Determine This?
- Mendel's data showed a near 3:1 ratio for all traits.
Character and F₂ Ratio
- Mendel looked at various traits, which yielded ratios close to, but not exactly, 3:1.
How Did Mendel Determine This? (cont.)
- The observed ratios were not exactly 3:1.
- The question raised was if the ratios were "close enough."
Binomial Distribution
- The probability of offspring phenotypes changes based on how many offspring are produced.
Question: Deviation from Expectations
- How to determine if deviation from expectation is due to chance or a causative factor.
Mendel's Experiment: Pp x Pp
- Data that shows the ratio of offspring compared to expected values.
Testing Mendel's First Law
- The principle of segregation explains how alleles separate during gamete formation and how offspring receive one allele per trait from each parent.
Mendel’s first Results/Conclusions
- Results of reciprocal crosses are always the same.
- F₁ individuals resemble only one parent.
- Missing trait in F₁ reappears in F₂ generation.
- alleles/factors segregate into gametes and reunite to re-form the new traits in offspring.
Mendel's Second Law: Principle of Independent Assortment
- Alleles of different genes independently assort.
- All allele combinations are equally likely.
Example: Flower Color and Plant Height
- Mendel’s second law discusses traits based on two separate genes.
What are the Possible Gametes?
- This question pertains to the formation of gametes having varying allele combinations.
Mendel’s Data from Dihybrid Cross?
- This section shows the observed and expected values of a dihybrid cross, examining the ratios of phenotypes and the test statistic.
Implications of Mendel's Laws
- Mendel's laws falsified the concept of blending inheritance.
- Genes/factors control inheritance.
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Description
Review of Mendel's principles, including segregation and independent assortment, originating from meiosis I and II. Key terms: gene, allele, genotype, and phenotype. Peas were chosen for genetics due to ease of growth, short generation times and categorical traits.