Mendel's Laws of Inheritance
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Questions and Answers

In a dihybrid cross, what phenotypic ratio is typically observed in the offspring, assuming independent assortment?

  • 3:1
  • 1:1:1:1
  • 9:3:3:1 (correct)
  • 1:2:1

What condition is essential for observing the classic 9:3:3:1 phenotypic ratio in a dihybrid cross?

  • One of the genes must exhibit dominance, while the other exhibits incomplete dominance.
  • The genes must be located close together on the same chromosome.
  • The genes must exhibit complete linkage.
  • The alleles at both genes must be assorting randomly with respect to each other. (correct)

What is the probability of producing a gamete with the genotype pt from an individual with the genotype PpTt, assuming independent assortment?

  • 1/2
  • 1/4 (correct)
  • 1/8
  • 1/16

According to Mendel's principle of independent assortment, how do alleles of different genes behave during gamete formation?

<p>They assort independently of each other. (B)</p> Signup and view all the answers

What is the expected frequency of each possible gamete combination from a dihybrid individual, assuming independent assortment?

<p>25% for each gamete type (A)</p> Signup and view all the answers

In a monohybrid cross (Pp x Pp), what is the probability of obtaining a homozygous recessive (pp) offspring?

<p>1/4 (D)</p> Signup and view all the answers

What does Mendel's Law of Segregation state?

<p>The two members of a gene pair (alleles) segregate (separate) from each other during gamete formation and are randomly distributed to the offspring. (A)</p> Signup and view all the answers

In the context of a Chi-Square test, what does it mean to 'reject the null hypothesis'?

<p>The observed data deviates significantly from the expected values, suggesting a factor other than chance is influencing the results. (D)</p> Signup and view all the answers

In a monohybrid cross where both parents are heterozygous (Pp), what is the expected phenotypic ratio of the offspring?

<p>3:1 (B)</p> Signup and view all the answers

A researcher performs a cross and observes a phenotypic ratio of 2.8:1. If the expected ratio is 3:1, what statistical test can be used to determine if the observed results are significantly different from the expected results?

<p>Chi-Square test (B)</p> Signup and view all the answers

If the Chi-Square test statistic ($χ^2$) is less than the critical value, what conclusion can be drawn?

<p>The null hypothesis cannot be rejected. (D)</p> Signup and view all the answers

In the context of genetic crosses, what is the significance of generating F1 offspring?

<p>Crossing F1 individuals allows observation of the segregation of alleles and recombination of traits in the F2 generation. (C)</p> Signup and view all the answers

A plant breeder crosses two pea plants and obtains the following results: 600 purple-flowered plants and 200 white-flowered plants. Based on this data, what is the approximate observed phenotypic ratio?

<p>3:1 (C)</p> Signup and view all the answers

In Mendel's dihybrid cross experiment, what phenotypic ratio is expected in the offspring if the genes assort independently?

<p>9:3:3:1 (A)</p> Signup and view all the answers

What is the purpose of calculating a chi-square ($\chi^2$) value in the context of Mendel's experiments?

<p>To assess the probability that observed results deviate from expected results by chance alone. (C)</p> Signup and view all the answers

In Mendel's dihybrid cross data, if the calculated $\chi^2$ value is 4.13 with 3 degrees of freedom, and the corresponding p-value is 0.25, what conclusion can be drawn?

<p>The observed results do not significantly differ from the expected results. (D)</p> Signup and view all the answers

Mendel's laws of inheritance directly refuted which prevailing idea about heredity?

<p>The idea of blending inheritance. (A)</p> Signup and view all the answers

In a dihybrid cross involving two traits, if the observed numbers of offspring phenotypes are purple/tall (372), purple/short (113), white/tall (126), and white/short (29) out of a total of 640, which calculation is used to determine the expected number of purple/tall offspring, assuming independent assortment?

<p>$\frac{9}{16} \times 640$ (D)</p> Signup and view all the answers

Which process directly leads to the segregation of alleles, as described by Mendel's Law of Segregation?

<p>Meiosis I and II during gamete formation. (A)</p> Signup and view all the answers

In a monohybrid cross, if the F1 generation consistently displays the trait of only one parent, which principle is being demonstrated?

<p>Principle of Uniformity of F1 (B)</p> Signup and view all the answers

A plant has the genotype AaBb for two independently assorting genes. What proportion of its gametes will be Ab, assuming independent assortment?

<p>1/4 (A)</p> Signup and view all the answers

What is the expected outcome of a reciprocal cross?

<p>The expression of the same traits in both sexes, regardless of which parent contributed which trait. (B)</p> Signup and view all the answers

In the context of quantitative traits, which equation best represents the relationship between phenotype, genotype, and environment?

<p>Phenotype = Genotype + Environment + (Genotype x Environment) (D)</p> Signup and view all the answers

Mendel chose pea plants for his experiments for several reasons. Which of the following is NOT one of the reasons?

<p>They have a long generation time. (C)</p> Signup and view all the answers

If a plant with the genotype 'Aa' self-pollinates, and 'A' is dominant for purple flowers while 'a' is recessive for white flowers, what proportion of the F1 generation would you expect to have white flowers?

<p>25% (C)</p> Signup and view all the answers

What is the most accurate definition of an allele?

<p>A variant form of a gene. (B)</p> Signup and view all the answers

In Mendelian genetics, what distinguishes alleles from genes?

<p>Alleles are alternative forms of a gene that control specific traits. (B)</p> Signup and view all the answers

What is the primary difference between the F1 and F2 generations in Mendelian genetics?

<p>The F1 generation results from a cross between true-breeding parental strains, while the F2 generation results from self-pollination of the F1 generation. (B)</p> Signup and view all the answers

Which of the following best describes Mendel's approach to studying inheritance?

<p>Isolating true-breeding strains and tracking single traits across generations. (A)</p> Signup and view all the answers

If 'D' represents the dominant allele for tall plants and 'd' represents the recessive allele for dwarf plants, what genotypes would be considered homozygous?

<p>DD and dd (B)</p> Signup and view all the answers

Suppose a researcher is studying a Mendelian trait in pea plants and observes a 3:1 phenotypic ratio in the F2 generation. What can they infer about the genotypes of the F1 generation?

<p>All F1 plants are heterozygous. (C)</p> Signup and view all the answers

If a true-breeding tall plant (TT) is crossed with a true-breeding dwarf plant (tt), what will be the genotype of the F1 generation?

<p>Tt (D)</p> Signup and view all the answers

A pea plant with round seeds (RR) is crossed with a pea plant with wrinkled seeds (rr). All the F1 generation plants have round seeds. If two plants from the F1 generation are crossed, what is the probability of obtaining a plant with wrinkled seeds in the F2 generation?

<p>25% (D)</p> Signup and view all the answers

In the context of Mendelian genetics, what does the reappearance of a trait in the F2 generation that was absent in the F1 generation indicate?

<p>The trait was present in the F1 generation as a recessive allele and reappears when two recessive alleles combine in the F2 generation. (A)</p> Signup and view all the answers

In a chi-square test, what does a high P-value (e.g., 0.54) indicate regarding the null hypothesis?

<p>There is a high probability of observing the obtained deviation or more extreme deviations by chance if the null hypothesis is true. (B)</p> Signup and view all the answers

How is the number of degrees of freedom (df) calculated in a chi-square test?

<p>df = Number of classes of data - 1 (B)</p> Signup and view all the answers

A researcher performs a cross between two pea plants with the genotype Pp. They observe 650 purple flowers and 270 white flowers. Using a chi-square test, they calculate a χ2 value of 2.5 and have 1 degree of freedom. Based on a critical χ2 value of 3.841 (p=0.05), what conclusion can they draw?

<p>Accept the null hypothesis; there is no significant difference between observed and expected results. (D)</p> Signup and view all the answers

What does Mendel's Principle of Segregation state?

<p>Alleles separate during gamete formation and randomly reunite during fertilization. (D)</p> Signup and view all the answers

What is the purpose of performing reciprocal crosses in genetics?

<p>To determine if the inheritance of a trait is sex-linked. (C)</p> Signup and view all the answers

Mendel's work disproved which of the following pre-existing ideas about inheritance?

<p>The idea of blending inheritance, where traits mix to form intermediate characteristics. (B)</p> Signup and view all the answers

How many alleles are present in a gamete for a single trait?

<p>one (A)</p> Signup and view all the answers

Mendel crossed true-breeding plants with different traits, and noted that the F1 generation resembled only one of the parents. What does this observation suggest?

<p>The trait that appears in the F1 generation is dominant. (A)</p> Signup and view all the answers

Flashcards

Principle of Segregation

Alleles for a gene are separated during gamete formation and reunite during fertilization.

Principle of Independent Assortment

The alleles of different genes assort independently during gamete formation.

Dihybrid Cross

A genetic cross that examines the inheritance of two different traits simultaneously.

Gametes Frequency 1/4

In a dihybrid cross, each gamete combination has an equal chance of occurring, specifically 1/4.

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9:3:3:1 Ratio

The expected phenotypic ratio in a dihybrid cross if genes assort independently.

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Law of Segregation

The two alleles for a gene separate during gamete formation.

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Law of Independent Assortment

Alleles of different genes segregate independently during gamete formation.

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Gene

The basic unit of heredity located on DNA.

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Allele

A variant form of a gene, representing different traits.

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Genotype

The genetic makeup of an organism that determines traits.

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Phenotype

The observable characteristics or traits of an organism.

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Mendelian Traits

Traits that follow the simple patterns of inheritance Mendel studied.

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Quantitative Traits

Traits influenced by both genotype and environment (G x E).

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Punnett Square

A diagram used to predict the genotype of offspring from parents' alleles.

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Monohybrid Cross

A genetic cross involving one trait controlled by a single gene with two alleles.

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F1 Generation

The first filial generation resulting from a cross of parents with different traits.

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F2 Generation

The second filial generation resulting from self-pollination of F1 individuals.

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Phenotypic Ratio

The ratio of different phenotypes in the offspring from a genetic cross.

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Chi-Square Test

A statistical test to compare observed data with expected data to see if differences are significant.

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3:1 Ratio

The expected phenotypic ratio for a monohybrid cross of heterozygous parents.

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Homozygous

Having two identical alleles for a trait (e.g., PP or pp).

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Heterozygous

Having one of each allele for a trait (e.g., Pp).

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Dominant vs. Recessive Alleles

Dominant alleles are represented by capital letters; recessive by lowercase.

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Mendel's Dihybrid Cross

An experiment that examined the inheritance of two traits, showing that alleles assort independently.

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Observations vs. Expectations

Comparing observed phenotypic counts to expected counts based on ratios.

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Chi-Square (χ2) Test

A statistical method to determine if observed results significantly differ from expected results.

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Independent Assortment

Mendel's principle stating alleles of different genes assort independently during gamete formation.

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Null Hypothesis (H0)

A statement that there is no difference between observed and expected values.

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Observed vs. Expected

Observed values are what you get from an experiment; expected values are predicted outcomes.

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Chi-Square (χ2)

A statistical test used to determine how expected values compare to observed values.

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Degrees of Freedom

Calculated as the number of categories minus one, used in statistical analysis.

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P-value

The probability of obtaining a result as extreme as observed if H0 is true.

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Mendel's Principle of Segregation

Alleles separate into gametes and can reunite randomly in offspring.

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Mendel's Principle of Independent Assortment

Alleles of different genes assort independently, giving equal chances of combinations.

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Reciprocal Crosses

Crosses between two parents that show the same results regardless of parental role.

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Study Notes

Mendel's Principles/Laws

  • Mendel's principles, encompassing segregation and independent assortment, arise from meiosis I and II.
  • Mendel lacked knowledge of meiosis; subsequent researchers, using microscopes, connected chromosome movement with the inheritance of traits.

Review of Terms

  • Gene: The fundamental unit of heredity, located on DNA (except RNA viruses).
  • Allele: A variant form of a gene.
  • Genotype: An organism's genetic makeup.
  • Phenotype: Observable characteristics of an organism.
  • Phenotype = Genotype + Environment + G X E.
  • For Mendelian traits, Phenotype = Genotype.

Why Peas?

  • Peas are easy to grow and produce many seeds.
  • They have short generation times.
  • Peas readily self-fertilize and can be cross-fertilized.
  • Peas exhibit many categorical traits (traits not influenced by the environment).

How to Cross a Pea Plant

  • Flowers are perfect.
  • Remove stamens to prevent self-pollination.
  • Collect pollen from another flower.
  • Cross-fertilize using the pollen to reproduce the plant.
  • Observe offspring phenotypes.

Mendel's Experiments

  • Mendel looked at seven pea plant traits.
  • He isolated true-breeding strains.
  • He studied each trait separately.
  • He designed careful breeding experiments.
  • He diligently recorded his results.
  • He used large sample sizes.
  • He followed traits across multiple generations.

Mendel's 1st Law: Principle of Segregation

  • The two alleles (members of a gene pair) separate during gamete formation.
  • Alleles are randomly distributed to offspring; each offspring receives one allele from each parent.

Mendel's Principle of Segregation (Terminology)

  • P Generation: Parental generation.
  • F₁ Generation: First filial generation.
  • F₂ Generation: Second filial generation.
  • F₃, F₄, F₅: Subsequent generations.
  • Monohybrid Cross: A mating between true-breeding strains that differ in one trait.

Terminology Continued: Reciprocal Crosses

  • Reciprocal crosses are when the parental traits are switched from the parent flower to the other.
  • For example the pollen of a purple flower is used to fertilize a white flower, then the pollen of a white flower is used to fertilize a purple flower. This is to see if the trait difference is between the pollen or the seed.

Principle of Uniformity of F₁

  • F₁ offspring from a monohybrid cross resemble one parent (and each other).

1 Trait Disappears in the F₁

  • One trait disappears in F₁ individuals.

Traits Reappear in F₂

  • The missing trait appears in the F₂ generation.
  • Mendel correctly reasoned that the information to produce the trait was present in the F₁ generation in the form of "factors" (now called genes) and was not blended.

Letters used to Designate Alleles

  • Dominant allele is represented using uppercase letters (e.g., P).
  • Recessive allele is represented using lowercase letters (e.g., p).

Heterozygous vs. Homozygous

  • Homozygotes have two copies of the same allele (e.g., PP or pp).
  • Heterozygotes have one copy of each allele (e.g., Pp).

Same Flower Example

  • This represents the self-pollination and the F₁ generation.

Punnett Square: Let's Make an F₁

  • A Punnett square demonstrates a hypothetical crossing of a parental plant with PP and pp phenotypes (showing 100% of the offspring having a Pp phenotype).

Punnett Square: Monohybrid Cross

  • A monohybrid cross is the mating of parental individuals that differ only in one character trait.

Punnett Square: Monohybrid Cross (Crossing F1 Individuals)

  • A Punnett square for a monohybrid cross.

If Factors Segregate

  • If factors segregate in F₁, then the expected phenotypic ratio in offspring is 3:1.

How Did Mendel Determine This?

  • Mendel's data showed a near 3:1 ratio for all traits.

Character and F₂ Ratio

  • Mendel looked at various traits, which yielded ratios close to, but not exactly, 3:1.

How Did Mendel Determine This? (cont.)

  • The observed ratios were not exactly 3:1.
  • The question raised was if the ratios were "close enough."

Binomial Distribution

  • The probability of offspring phenotypes changes based on how many offspring are produced.

Question: Deviation from Expectations

  • How to determine if deviation from expectation is due to chance or a causative factor.

Mendel's Experiment: Pp x Pp

  • Data that shows the ratio of offspring compared to expected values.

Testing Mendel's First Law

  • The principle of segregation explains how alleles separate during gamete formation and how offspring receive one allele per trait from each parent.

Mendel’s first Results/Conclusions

  • Results of reciprocal crosses are always the same.
  • F₁ individuals resemble only one parent.
  • Missing trait in F₁ reappears in F₂ generation.
  • alleles/factors segregate into gametes and reunite to re-form the new traits in offspring.

Mendel's Second Law: Principle of Independent Assortment

  • Alleles of different genes independently assort.
  • All allele combinations are equally likely.

Example: Flower Color and Plant Height

  • Mendel’s second law discusses traits based on two separate genes.

What are the Possible Gametes?

  • This question pertains to the formation of gametes having varying allele combinations.

Mendel’s Data from Dihybrid Cross?

  • This section shows the observed and expected values of a dihybrid cross, examining the ratios of phenotypes and the test statistic.

Implications of Mendel's Laws

  • Mendel's laws falsified the concept of blending inheritance.
  • Genes/factors control inheritance.

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Review of Mendel's principles, including segregation and independent assortment, originating from meiosis I and II. Key terms: gene, allele, genotype, and phenotype. Peas were chosen for genetics due to ease of growth, short generation times and categorical traits.

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