10 Questions
What is the purpose of a hydropathy plot in protein sequences?
To locate the membrane-spanning helices
How is lateral diffusion in membranes defined?
The constant motion of lipids and membrane proteins
What does a peak of +84 kJ mol−1 or more indicate on a hydropathy plot?
Possible membranespanning α helix
How are biological membranes described in the text?
Dynamic and not rigid
Which process can be visualized for proteins using FRAP?
Lateral diffusion
What technique involves labeling a cell-surface component with a fluorescent chromophore?
FRAP
What is destroyed (bleached) during the FRAP process?
Fluorescent molecules in a region
How are lipids and many membrane proteins constantly moving in membranes?
By visualizing lateral diffusion
What does a high FRAP activity imply?
Constant motion of lipids and proteins
What is the main purpose of fluorescence recovery after photobleaching (FRAP)?
Visualize lateral diffusion of lipids and proteins
Test your knowledge on the melting temperature of phosphatidylcholine with different pairs of identical fatty acid chains. The quiz covers the relationship between fatty acid chain length, saturation, and the resulting melting temperature based on Table 12.3.
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