Podcast
Questions and Answers
What is the purpose of a hydropathy plot in protein sequences?
What is the purpose of a hydropathy plot in protein sequences?
- To determine the lateral diffusion rate
- To locate the membrane-spanning helices (correct)
- To visualize FRAP
- To measure the melting temperature of phosphatidylcholine
How is lateral diffusion in membranes defined?
How is lateral diffusion in membranes defined?
- The constant motion of lipids and membrane proteins (correct)
- The process of visualizing FRAP
- The destruction of fluorescent molecules
- The melting process of phosphatidylcholine
What does a peak of +84 kJ mol−1 or more indicate on a hydropathy plot?
What does a peak of +84 kJ mol−1 or more indicate on a hydropathy plot?
- High FRAP activity
- Presence of porin proteins
- Possible membranespanning α helix (correct)
- Stable membrane fluidity
How are biological membranes described in the text?
How are biological membranes described in the text?
Which process can be visualized for proteins using FRAP?
Which process can be visualized for proteins using FRAP?
What technique involves labeling a cell-surface component with a fluorescent chromophore?
What technique involves labeling a cell-surface component with a fluorescent chromophore?
What is destroyed (bleached) during the FRAP process?
What is destroyed (bleached) during the FRAP process?
How are lipids and many membrane proteins constantly moving in membranes?
How are lipids and many membrane proteins constantly moving in membranes?
What does a high FRAP activity imply?
What does a high FRAP activity imply?
What is the main purpose of fluorescence recovery after photobleaching (FRAP)?
What is the main purpose of fluorescence recovery after photobleaching (FRAP)?