Medical Protozoology: Features, Structure

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Questions and Answers

Considering the evolutionary pressures on parasitic protozoa, which adaptation would MOST likely arise in a species exhibiting exclusively asexual reproduction within a single host?

  • Heightened sensitivity to environmental cues signaling host stress to trigger encystation and dormancy.
  • Specialized surface ligands for evading antibody recognition while maintaining host cell adhesion. (correct)
  • Enhanced genetic recombination mechanisms to combat host immune responses.
  • Increased reliance on complex life cycles involving multiple hosts to maximize transmission opportunities.

Given the diverse mechanisms of protozoan motility, which statement BEST describes the evolutionary trade-offs associated with each method?

  • Pseudopodia offer rapid bursts of speed useful in high-predation environments, at the cost of energy efficiency.
  • Apicomplexan gliding motility maximizes speed and minimizes energy consumption but renders them ineffective in complex environments.
  • Flagella are ideal for traversing mucosal barriers but are energetically inefficient in nutrient-poor environments.
  • Cilia provide high maneuverability and efficient nutrient uptake, but are susceptible to viscous drag in dense tissues. (correct)

In the context of protozoan nutrition, what functional implications arise from the presence or absence of a cytostome in different parasitic species?

  • The presence of a cytostome is directly correlated with the complexity of the protozoan's life cycle, indicating a higher degree of host specialization.
  • The absence of a cytostome necessitates a strictly osmotrophic mode of nutrition, limiting the range of potential host tissues that can be colonized.
  • Protozoa with cytostomes exhibit greater metabolic flexibility, capable of both phagocytosis and absorption of dissolved nutrients from the extracellular environment. (correct)
  • Protozoa lacking a cytostome are obligate intracellular parasites, relying exclusively on direct nutrient uptake from the host cell cytoplasm.

How does the differential distribution of chromatin within the nucleus of protozoa contribute to adaptive strategies in the face of varying environmental stresses?

<p>Chromatin distribution affects gene expression, influencing the ability of protozoa to regulate metabolism under stress. (C)</p> Signup and view all the answers

In complex protozoan life cycles involving multiple hosts, what selective advantages are conferred by alternating between asexual and sexual reproduction?

<p>Asexual reproduction in the vertebrate host ensures efficient clonal expansion, while sexual reproduction in the invertebrate vector promotes genetic diversity aiding in adaptation. (D)</p> Signup and view all the answers

Considering the diverse strategies protozoa employ for excretion, which scenario BEST illustrates the adaptive significance of contractile vacuoles?

<p>Contractile vacuoles enable protozoa to regulate cell turgor and expel waste products in freshwater environments. (C)</p> Signup and view all the answers

Given the various modes of protozoan transmission, which strategy would be MOST effective in preventing the spread of a parasite with exclusively transplacental transmission?

<p>Screening pregnant individuals for parasitic infections and providing targeted treatment to prevent vertical transmission. (C)</p> Signup and view all the answers

Considering the classification of protozoa, what are the KEY evolutionary distinctions between Sarcodina and Mastigophora that underlie their diverse parasitic strategies?

<p>Sarcodina utilize pseudopodia for locomotion and phagocytosis, enabling them to thrive as free-living amoebae and opportunistic parasites, whereas Mastigophora use flagella and are more specialized intestinal or urogenital parasites. (B)</p> Signup and view all the answers

In the context of protozoan secretions, what advantage is conferred by the production of digestive and proteolytic enzymes by parasitic species?

<p>Secretion of digestive and proteolytic enzymes allows for extracellular digestion and nutrient acquisition, effectively increasing the range of exploitable hosts and tissues. (B)</p> Signup and view all the answers

Given protozoan secretion capacity, which aspect of protozoan biology is MOST enhanced by secretion of toxic and antigenic substances?

<p>The manipulation of host immune responses to establish chronic infections. (A)</p> Signup and view all the answers

Flashcards

What are Protozoa?

Single-celled eukaryotic microorganisms that perform various functions like reproduction, digestion, and respiration.

What is Ectoplasm?

The outer, homogenous layer of cytoplasm in protozoa, used for locomotion, food engulfment and protection.

What is Endoplasm?

The inner, granular part of cytoplasm containing nucleus, golgi bodies and food vacuoles. Functions include nutrition and reproduction

How do protozoa move?

Protozoa can move via pseudopodia, cilia, or flagella, each facilitating movement in different ways.

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What is Binary Fission?

Asexual reproduction where a parasite divides into two or more equal parts.

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What is Multiple Fission?

Asexual reproduction of a parasite where the nucleus divides several times before the cell divides.

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What is Endodyogeny?

A type of asexual reproduction where a parasite multiplies by internal budding, forming two daughter cells.

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What is a Trophozoite?

The active, feeding, moving, and multiplying stage of a parasitic protozoan.

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What is a Cyst?

The resting or resistant stage of a parasitic protozoan, often bounded by a tough cell wall.

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How do protozoa infect?

Ingestion, eating improperly cooked meat, insect bites, sexual, transplacental transmission and autoinfection.

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Study Notes

Introduction to Medical Protozoology Learning Objectives

  • Recognize general features and structure of protozoa
  • Explain biological processes in protozoa
  • Recognize life cycle of protozoa
  • Enlist modes of transmission of protozoa and their control measures
  • Outline classification of protozoa

Protozoa

  • Single-celled eukaryotic microorganisms
  • Perform all functions including reproduction, digestion, excretion, and respiration
  • Derives from the Greek words "protos" (first) and "zoon" (animal)
  • Parasitic protozoa adapt to different host species

General Features of Protozoa

  • Most protozoa are non-pathogenic
  • Opportunistic protozoa causes severe disease in immunocompromised individuals
  • Some protozoa cause major diseases such as malaria
  • Exhibit a wide range of sizes (1 - 150 μm), shapes, and structures

Structure of Protozoa

  • The protozoan cell is covered with a cell membrane
  • It consists of cytoplasm and a nucleus

Cytoplasm

  • Composed of ectoplasm and endoplasm

Ectoplasm

  • The outer homogeneous part
  • Functions as an organ for locomotion
  • Engulfs food using pseudopodia
  • Assists in respiration, ingestion, and waste discharge
  • Provides sensory and protective covering for the cell

Endoplasm

  • The inner granular portion
  • Contains the nucleus, golgi bodies, endoplasmic reticulum, and food vacuoles
  • Includes contractile vacuoles that regulate osmotic pressure
  • Functions in nutrition (metabolism) and reproduction

Nucleus

  • Usually single, but can be double or multiple
  • Some species have as many as 100 nuclei in a single cell
  • Contains one or more nucleoli or a central karyosome (DNA-containing body)
  • Chromatin can be distributed along the periphery
  • Essential for regulation of cell activity and reproduction

Biology of Protozoa: Movement

  • Protozoa moves by pseudopodia (Amoeba), cilia (Balantidium), and flagella (Giardia and Leishmania) with undulating membrane (Trypanosoma)
  • Other protozoa use an apical complex for host cell penetration instead of locomotion organs, such as spore-forming apicomplexan protozoa like Plasmodium, Toxoplasma, and Cryptosporidium

Biology of Protozoa: Respiration

  • Protozoa respires by either directly taking oxygen or through a metabolic process.

Biology of Protozoa: Nutrition

  • Absorbing liquid food
  • Ingesting solid food using pseudopodia or cytostome, with food vacuoles

Biology of Protozoa: Excretion

  • Performed by osmotic pressure, contractile vacuoles, diffusion, or cytopyge

Biology of Protozoa: Secretion

  • Protozoa secrete cyst walls, pigments, digestive and proteolytic enzymes, and toxic and antigenic substances

Biology of Protozoa: Reproduction

  • Asexual and sexual reproduction include;

Asexual Reproduction

  • Binary fission occurs when a parasite divides either longitudinally or transversally into two or more equal parts, such as in Amoeba and ciliates
  • In multiple fission, the nucleus undergoes several successive divisions (schizogony, merogony), for example, the malaria parasite (Plasmodium) in humans
  • Endodyogeny involves parasite multiplication by internal budding, resulting in the formation of two daughter cells, such as Toxoplasma

Sexual Reproduction

  • Conjugation occurs when two organisms join and reciprocally exchange nuclear material, as seen in Balantidium
  • Gametogony is when male and female gametocytes are produced, and after fertilization, the zygote gives rise to numerous sporozoites, as seen in the malaria parasite in mosquitoes

Life Cycle of Protozoa

  • Simple: Protozoa needs only one host within which they multiply asexually e.g. Amoeba
  • Complex: Protozoa multiply asexually in one host (human) and sexually in another host (mosquito) e.g. Malaria parasite

Life Stages of Protozoa

  • Trophozoite is the active, feeding, moving, and multiplying stage of the parasite.
  • Cyst is the resting or resistant stage of protozoa, bounded by a tough cell wall

Modes of Infection in Protozoa

  • Ingestion of the infective stage: Giardia intestinalis cyst, Entamoeba histolytica cyst, Cryptosporidium parvum oocyst, Toxoplasma gondii oocyst
  • Eating improperly cooked meat: Toxoplasma gondii cyst
  • Insect bite: Female Anopheles transmits malaria, female sand fly (Phlebotomus) transmits leishmaniasis, kissing bug (Reduviid or Triatomine) transmits Chagas disease
  • Sexual transmission: Trichomonas vaginalis trophozoite
  • Blood transfusion: Plasmodium trophozoites and merozoites
  • Organ transplantation: Leishmania donovani amastigote
  • Transplacental transmission: Toxoplasma gondii tachyzoites
  • Autoinfection: Can be either internal or external (Feco-oral transmission), e.g., Giardia lamblia, Entamoeba histolytica, Cryptosporidium parvum

Prevention and Control of Protozoa

  • Health education
  • Environmental sanitation and personal hygiene
  • Case treatment
  • Pure water supply
  • Safe and clean food
  • Sanitary disposal of feces
  • Vectors control

Classification of Protozoa

Phylum: Sarcomastigophora

  • Subphylum: Sarcodina (Amoebae) are parasitic and free-living amoebas
  • Subphylum: Mastigophora (Flagellates) can be intestinal (Giardia) or urogenital (Trichomonas)
  • Haemo-somatic: (Leishmania and Trypanosoma)

Phylum: Ciliophora

  • Balantidium

Phylum: Apicomplexa

  • Plasmodium, Toxoplasma & Cryptosporidium

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