Mechanosensation and PIEZO2 in Mice
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Questions and Answers

What is the significance of PIEZO2 in mechanosensation?

  • It is mainly responsible for pain perception.
  • It plays an essential role in perineal touch and sexual function. (correct)
  • It has no known function in mammals.
  • It primarily influences visual sensory pathways.

    • What behavioral test was used to assess penile protrusion in the study?

  • Social interaction test
  • Climbing test
  • Nest building test
  • Penile Protrusion Test (correct)

    • What deficits are caused by impaired PIEZO2-dependent mechanosensation?

  • Decreased muscle coordination.
  • Increased food intake.
  • Inhibited vision and hearing.
  • Impaired perigenital sensation and copulation. (correct)

    • What limitation did the study face regarding human subjects?

    <p>PIEZO2 deficiency is extremely rare.</p> Signup and view all the answers

    Which of the following is NOT a role associated with PIEZO2 in the study?

    <p>Facilitating balance.</p> Signup and view all the answers

    In what condition might PIEZO2 have therapeutic potential?

    <p>Hypo and hypersensitivity.</p> Signup and view all the answers

    What was a method used to evaluate mechanosensation in the study?

    <p>Behavioral assays.</p> Signup and view all the answers

    How was the functional role of PIEZO2 characterized in the study?

    <p>It was determined essential for perineal mechanosensation.</p> Signup and view all the answers

    What is the role of PIEZO2 in mice based on the study?

    <p>It is essential for discriminative touch.</p> Signup and view all the answers

    What behavioral responses were measured in the study when using von Frey filaments?

    <p>Withdrawal, flinch, and jump responses.</p> Signup and view all the answers

    Why is the perineal mechanosensation significant in this context?

    <p>It is essential for sexual function.</p> Signup and view all the answers

    What testing condition was used to measure responses to touch in mice?

    <p>Mice were habituated on a mesh floor covered by a transparent glass vessel.</p> Signup and view all the answers

    What was the method of stimulus application used in the study?

    <p>Direct application of nylon monofilaments.</p> Signup and view all the answers

    What kind of mutations were anticipated in the study regarding the PIEZO2 channel?

    <p>Non-functional mutations affecting touch discrimination.</p> Signup and view all the answers

    Which of the following best describes the mechanical sensitivity assay used in the experiment?

    <p>Single force touch from varying filament weights.</p> Signup and view all the answers

    What is the expected outcome if PIEZO2 is non-functional in mechanosensation?

    <p>Loss of touch sensitivity.</p> Signup and view all the answers

    What is the significance of PIEZO2 in mechanosensation?

    <p>It is essential for detecting gentle stimuli.</p> Signup and view all the answers

    How are HTMRs categorized based on their response to stimuli?

    <p>By responding primarily to pinch and/or high force von Frey filaments.</p> Signup and view all the answers

    What factor distinguishes air-puff cells in mechanosensation research?

    <p>Responses that are at least twice as strong as those to gentle brush stimuli.</p> Signup and view all the answers

    What does the formula for calculating dF/F represent?

    <p>Relative change in fluorescence over baseline fluorescence.</p> Signup and view all the answers

    In which way are LTMRs distinguished from HTMRs?

    <p>LTMRs respond to any gentle stimuli while HTMRs primarily to painful stimuli.</p> Signup and view all the answers

    What type of stimuli do High Threshold Mechanosensory Neurons (HTMRs) primarily respond to?

    <p>Pinch and high force von Frey filaments.</p> Signup and view all the answers

    What does the imaging of cellular responses likely indicate about the stimulus applied?

    <p>Higher intensity stimuli elicit stronger cellular responses.</p> Signup and view all the answers

    What aspect of mechanosensation is critical for sexual function?

    <p>The role of PIEZO2 and perineal mechanosensation.</p> Signup and view all the answers

    Study Notes

    Piezo Channels

    • PIEZO2 is a mechanically activated ion channel
    • PIEZO2 is essential for discriminative touch
    • Perineal mechanosensation is essential for sexual function in mice
    • PIEZO2 deficiency results in impaired perineal mechanosensation, impacting sexual function
    • Measuring behavioural sensitivity involves applying calibrated von Frey filaments to the hind-paw or perineum, observing responses like withdrawal, flinch, and jump
    • Threshold for sensitivity is determined by recording the force that elicits 5 out of 10 responses
    • PIEZO2 deficiency results in lower sensitivity thresholds compared to wild-type mice
    • Investigating the cellular response to mechanical stimulation involves measuring calcium activity in neurons using GCaMP6f, a fluorescent calcium indicator
    • Neurons are classified as High Threshold Mechanosensory Neurons (HTMRs) or Low Threshold Mechanosensory Neurons (LTMRs) based on their response to different stimuli
    • HTMRs respond mainly to pinch and high force von Frey filaments
    • LTMRs respond to gentle stimuli such as air puff and brush
    • Air-puff cells are identified as those that exhibit significantly higher responses to air-puff compared to brush stimuli
    • These findings have implications for understanding the role of PIEZO2 in human sexual function and potential therapeutic applications for hypo- and hypersensitivity disorders.

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    Lecture 5 Part 2 Mechanosenstions PDF

    Description

    This quiz explores the role of PIEZO2 as a mechanically activated ion channel in discriminative touch and its importance in perineal mechanosensation. It examines the impact of PIEZO2 deficiency on sexual function in mice, measuring sensitivity thresholds, and classifying neurons based on their mechanosensory responses.

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