20 Questions
What is the role of IF1 in the 30s complex formation?
It blocks the initiator tRNA from entering the 'A-site'
What is the role of IF2 in the 30s complex formation?
It places the initiator tRNA at P-site
Which initiation factors remain bound with the 30S subunit during 30s complex formation?
IF1 and IF3
What is the role of IF3 in the 30s complex formation?
It blocks binding of the 50s subunit with 30s subunit
What is the function of Streptomycin in inhibiting 30s complex formation?
It binds to the 16s rRNA in the 30S subunit and inhibits binding of the initiator tRNA with the start codon
What is the consequence of Streptomycin binding to the 16s rRNA in the 30S subunit?
Mis-reading of the template causes polypeptide frame shifting
What is the role of IF1 and IF2 in placing the Initiator tRNA to 'P-site'?
Both IF1 and IF2 help in placing the Initiator tRNA to 'P-site'
Which initiation factor blocks the initiator tRNA from entering the 'A-site'?
IF1
What is the consequence of mis-reading of the template during 30s complex formation?
Polypeptide frame shifting
What is the consequence of Streptomycin inhibiting binding of the initiator tRNA with the start codon?
Initiator tRNA is placed incorrectly
What is the function of IF1 in the 30s complex formation?
It blocks the initiator tRNA from entering the 'A-site'
Which initiation factor remains bound with the 30S subunit during the 30s complex formation?
IF3
What is the function of IF3 in the 30s complex formation?
It blocks binding of the 50s subunit with 30s subunit
Which initiation factor places the initiator tRNA (fmet-tRNA) at P-site during the 30s complex formation?
IF2
What is the role of both IF1 and IF2 in the 30s complex formation?
They place the initiator tRNA at P-site
Which antibiotic binds to the 16s rRNA in the 30S subunit and inhibits binding of the initiator tRNA with the start codon during the 30s complex formation?
Streptomycin
What is the consequence of streptomycin binding to the 16s rRNA during the 30s complex formation?
Initiator tRNA is placed incorrectly
Which initiation factor(s) help in blocking the binding of the 50s subunit with 30s subunit during the 30s complex formation?
IF3
Which initiation factor helps in placing the initiator tRNA to 'P-site' during the 30s complex formation?
IF2
Which initiation factor blocks the initiator tRNA from entering the 'A-site' during the 30s complex formation?
IF1
Test your knowledge on 30s complex formation and the three initiation factors required for it. Learn about the roles of IF1, IF2, and IF3 in placing the initiator tRNA in the correct position and how certain antibiotics can inhibit this process. Dive into the details of molecular biology and challenge yourself with this quiz.
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