30 Questions
Which of the following is NOT a field resulting from biotechnology?
Bioinformatics
What is the purpose of DNA gel electrophoresis?
To separate DNA fragments by size
Which enzyme is responsible for joining DNA molecules covalently?
DNA ligase
What is the main application of gene therapy?
Correcting genetic defects
Which of the following is NOT a common restriction enzyme?
Reverse transcriptase
Which enzyme is responsible for creating a recombinant DNA molecule?
DNA ligase
Which technique did Mary-Claire King use to match children with their relatives in Argentina during the 'Dirty War'?
Mitochondrial DNA analysis
What is the purpose of a vector in DNA cloning?
To generate recombinant molecules
What is the main focus of biotechnology today?
Manipulating DNA
What is the main function of a selectable marker in a vector?
To eliminate cells that have not taken up the vector
What is the purpose of genetic engineering?
To alter yeast and mammalian cells growing in culture
What is the purpose of reverse transcriptase in cloning eukaryotic genes into bacteria?
To obtain a copy of DNA that lacks introns
What is the role of microbiological and biochemical techniques in biotechnology?
To solve practical problems
What is the main application of CRISPR-Cas technologies?
Gene editing
What is the significance of Mary-Claire King's use of mitochondrial DNA analysis in Argentina?
It allowed her to match children with their relatives
Which enzyme is used in PCR to synthesize cDNA from mRNA template?
Reverse transcriptase
What is the purpose of qPCR?
To determine the relative amount of target DNA in a sample
What does FISH stand for?
Fluorescence In Situ Hybridization
What is the purpose of colony blotting?
To detect specific DNA sequence in colonies grown on agar plates
What is the function of DNA microarrays?
To study gene expression in organisms
Which of the following is NOT a characteristic of dCas9 in gene function studies?
It cuts DNA
What are the two components that diagnostic kits for infectious diseases or cancers using CRISPR-Cas technologies rely on?
Cas nuclease and RNA guide
What is the name of the project that aimed to sequence all known animal, plant, protozoan, and fungal species?
The Earth BioGenome Project
What is the process called when computers align and merge data from millions of overlapping small DNA fragments to create an entire sequence?
Sequence assembly
Which sequencing method uses long fragments of DNA passed through a microscopic pore where different nucleotides block electric current to varying degrees?
Nanopore Sequencing
Which of the following accurately describes the process of gene expression?
mRNA is isolated from an organism and used to synthesize labeled, single stranded cDNA
What is the purpose of hybridization in the context of microarray analysis?
To anneal fragments complementary to the oligonucleotides
Which of the following is a potential concern regarding genetic engineering?
Creation of new infectious agents for bioterrorism
What is the purpose of the Recombinant DNA Advisory Committee (RAC)?
To develop guidelines for using recombinant DNA and cloning
What are some concerns related to genetic changes from CRISPR/Cas9 technologies?
Difficulties in detecting genetic changes
Test your knowledge on the use of "Dead" or dCas9 in gene function studies. Learn about its ability to bind to specific DNA sequences, block RNA polymerase, deliver molecules, activate genes, and locate genes using fluorescent markers.
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