Mastering the Power of "Dead" or dCas9
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Questions and Answers

Which of the following is NOT a field resulting from biotechnology?

  • Proteomics
  • Transcriptomics
  • Genomics
  • Bioinformatics (correct)

    • What is the purpose of DNA gel electrophoresis?

  • To synthesize complementary DNA strands
  • To create recombinant DNA molecules
  • To separate DNA fragments by size (correct)
  • To analyze gene expression

    • Which enzyme is responsible for joining DNA molecules covalently?

  • Restriction enzyme
  • Reverse transcriptase
  • DNA polymerase
  • DNA ligase (correct)

    • What is the main application of gene therapy?

    <p>Correcting genetic defects</p> Signup and view all the answers

    Which of the following is NOT a common restriction enzyme?

    <p>Reverse transcriptase</p> Signup and view all the answers

    Which enzyme is responsible for creating a recombinant DNA molecule?

    <p>DNA ligase</p> Signup and view all the answers

    Which technique did Mary-Claire King use to match children with their relatives in Argentina during the 'Dirty War'?

    <p>Mitochondrial DNA analysis</p> Signup and view all the answers

    What is the purpose of a vector in DNA cloning?

    <p>To generate recombinant molecules</p> Signup and view all the answers

    What is the main focus of biotechnology today?

    <p>Manipulating DNA</p> Signup and view all the answers

    What is the main function of a selectable marker in a vector?

    <p>To eliminate cells that have not taken up the vector</p> Signup and view all the answers

    What is the purpose of genetic engineering?

    <p>To alter yeast and mammalian cells growing in culture</p> Signup and view all the answers

    What is the purpose of reverse transcriptase in cloning eukaryotic genes into bacteria?

    <p>To obtain a copy of DNA that lacks introns</p> Signup and view all the answers

    What is the role of microbiological and biochemical techniques in biotechnology?

    <p>To solve practical problems</p> Signup and view all the answers

    What is the main application of CRISPR-Cas technologies?

    <p>Gene editing</p> Signup and view all the answers

    What is the significance of Mary-Claire King's use of mitochondrial DNA analysis in Argentina?

    <p>It allowed her to match children with their relatives</p> Signup and view all the answers

    Which enzyme is used in PCR to synthesize cDNA from mRNA template?

    <p>Reverse transcriptase</p> Signup and view all the answers

    What is the purpose of qPCR?

    <p>To determine the relative amount of target DNA in a sample</p> Signup and view all the answers

    What does FISH stand for?

    <p>Fluorescence In Situ Hybridization</p> Signup and view all the answers

    What is the purpose of colony blotting?

    <p>To detect specific DNA sequence in colonies grown on agar plates</p> Signup and view all the answers

    What is the function of DNA microarrays?

    <p>To study gene expression in organisms</p> Signup and view all the answers

    Which of the following is NOT a characteristic of dCas9 in gene function studies?

    <p>It cuts DNA</p> Signup and view all the answers

    What are the two components that diagnostic kits for infectious diseases or cancers using CRISPR-Cas technologies rely on?

    <p>Cas nuclease and RNA guide</p> Signup and view all the answers

    What is the name of the project that aimed to sequence all known animal, plant, protozoan, and fungal species?

    <p>The Earth BioGenome Project</p> Signup and view all the answers

    What is the process called when computers align and merge data from millions of overlapping small DNA fragments to create an entire sequence?

    <p>Sequence assembly</p> Signup and view all the answers

    Which sequencing method uses long fragments of DNA passed through a microscopic pore where different nucleotides block electric current to varying degrees?

    <p>Nanopore Sequencing</p> Signup and view all the answers

    Which of the following accurately describes the process of gene expression?

    <p>mRNA is isolated from an organism and used to synthesize labeled, single stranded cDNA</p> Signup and view all the answers

    What is the purpose of hybridization in the context of microarray analysis?

    <p>To anneal fragments complementary to the oligonucleotides</p> Signup and view all the answers

    Which of the following is a potential concern regarding genetic engineering?

    <p>Creation of new infectious agents for bioterrorism</p> Signup and view all the answers

    What is the purpose of the Recombinant DNA Advisory Committee (RAC)?

    <p>To develop guidelines for using recombinant DNA and cloning</p> Signup and view all the answers

    What are some concerns related to genetic changes from CRISPR/Cas9 technologies?

    <p>Difficulties in detecting genetic changes</p> Signup and view all the answers

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