Mastering the Power of "Dead" or dCas9

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Questions and Answers

Which of the following is NOT a field resulting from biotechnology?

Proteomics
Transcriptomics
Genomics
Bioinformatics (correct)

What is the purpose of DNA gel electrophoresis?

To synthesize complementary DNA strands
To create recombinant DNA molecules
To separate DNA fragments by size (correct)
To analyze gene expression

Which enzyme is responsible for joining DNA molecules covalently?

Restriction enzyme
Reverse transcriptase
DNA polymerase
DNA ligase (correct)

What is the main application of gene therapy?

Correcting genetic defects (D) Signup and view all the answers

Which of the following is NOT a common restriction enzyme?

Reverse transcriptase (C) Signup and view all the answers

Which enzyme is responsible for creating a recombinant DNA molecule?

DNA ligase (C) Signup and view all the answers

Which technique did Mary-Claire King use to match children with their relatives in Argentina during the 'Dirty War'?

Mitochondrial DNA analysis (D) Signup and view all the answers

What is the purpose of a vector in DNA cloning?

To generate recombinant molecules (D) Signup and view all the answers

What is the main focus of biotechnology today?

Manipulating DNA (B) Signup and view all the answers

What is the main function of a selectable marker in a vector?

To eliminate cells that have not taken up the vector (C) Signup and view all the answers

What is the purpose of genetic engineering?

To alter yeast and mammalian cells growing in culture (A) Signup and view all the answers

What is the purpose of reverse transcriptase in cloning eukaryotic genes into bacteria?

To obtain a copy of DNA that lacks introns (B) Signup and view all the answers

What is the role of microbiological and biochemical techniques in biotechnology?

To solve practical problems (B) Signup and view all the answers

What is the main application of CRISPR-Cas technologies?

Gene editing (B) Signup and view all the answers

What is the significance of Mary-Claire King's use of mitochondrial DNA analysis in Argentina?

It allowed her to match children with their relatives (B) Signup and view all the answers

Which enzyme is used in PCR to synthesize cDNA from mRNA template?

Reverse transcriptase (B) Signup and view all the answers

What is the purpose of qPCR?

To determine the relative amount of target DNA in a sample (D) Signup and view all the answers

What does FISH stand for?

Fluorescence In Situ Hybridization (C) Signup and view all the answers

What is the purpose of colony blotting?

To detect specific DNA sequence in colonies grown on agar plates (A) Signup and view all the answers

What is the function of DNA microarrays?

To study gene expression in organisms (C) Signup and view all the answers

Which of the following is NOT a characteristic of dCas9 in gene function studies?

It cuts DNA (A) Signup and view all the answers

What are the two components that diagnostic kits for infectious diseases or cancers using CRISPR-Cas technologies rely on?

Cas nuclease and RNA guide (A) Signup and view all the answers

What is the name of the project that aimed to sequence all known animal, plant, protozoan, and fungal species?

The Earth BioGenome Project (B) Signup and view all the answers

What is the process called when computers align and merge data from millions of overlapping small DNA fragments to create an entire sequence?

Sequence assembly (D) Signup and view all the answers

Which sequencing method uses long fragments of DNA passed through a microscopic pore where different nucleotides block electric current to varying degrees?

Nanopore Sequencing (D) Signup and view all the answers

Which of the following accurately describes the process of gene expression?

mRNA is isolated from an organism and used to synthesize labeled, single stranded cDNA (D) Signup and view all the answers

What is the purpose of hybridization in the context of microarray analysis?

To anneal fragments complementary to the oligonucleotides (A) Signup and view all the answers

Which of the following is a potential concern regarding genetic engineering?

Creation of new infectious agents for bioterrorism (D) Signup and view all the answers

What is the purpose of the Recombinant DNA Advisory Committee (RAC)?

To develop guidelines for using recombinant DNA and cloning (D) Signup and view all the answers

What are some concerns related to genetic changes from CRISPR/Cas9 technologies?

Difficulties in detecting genetic changes (D) Signup and view all the answers

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