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Questions and Answers
Which temperature range should reagents be kept at?
How should PCR tubes with tablets be stored for more than 3 weeks?
How long can the solution with protease added to the lysis buffer be stored in the fridge?
How long can diluted lysing agent 1 (24E assay) be stored at 20-30°C?
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How long can the mixture of diluted lysing agent 1 and lysing agent 2 be used?
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How long can cooling blocks be used after removal from the fridge?
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How long can unopened lysates be stored when refrigerated at 4°C?
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Where should sample preparation be done for the BAX Applications Project?
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What should be done when doing BAX application projects?
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Where can guidelines for validated food products be found?
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What should be done to prepare bacterial cultures for MicroSnap/InSite?
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What should be done before running BAX kits?
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When should PCR tubes be hydrated for accurate results?
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What should be done when running the full process of BAX kits?
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Where can guidance on running a RiboPrinter assay be found?
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Reagents should be stored in the freezer to extend their shelf life.
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The mylar bag of PCR tubes should be sealed with desiccant if storing for more than 3 weeks.
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The protease solution can be stored in the fridge for up to 2 weeks.
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Diluted lysing agent 1 (24E assay) can be stored at room temperature for up to 6 months.
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The mixture of diluted lysing agent 1 and lysing agent 2 must be used within 4 hours.
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Cooling blocks should be refrigerated and used within 30 minutes of removal from the fridge.
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Opened lysates can be stored for up to one week when frozen at -20°C.
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The BAX method should always be run without running a reference detection method alongside it.
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ISO guidelines for food product testing can be found in the BAX system User Guide for each BAX kit.
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To minimize contamination, lysis steps for BAX kits should be performed in the PCR workstation hood or biosafety cabinet.
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PCR tubes should be hydrated before loading them into the instrument to ensure accurate results.
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All standard and 24E kits can be run simultaneously in the BAX instrument.
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The RiboPrinter can be used to obtain an ID for cultures isolated through the reference method.
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The BAX results should be saved into the project folder after downloading them from the BAX computer.
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A report summarizing the key findings should be submitted for approval by the Applications Manager after completing the project.
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