Mastering Reagent Storage
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Questions and Answers

Which temperature range should reagents be kept at?

  • -20°C
  • 2-8°C (correct)
  • 4°C
  • 20-30°C
  • How should PCR tubes with tablets be stored for more than 3 weeks?

  • In a mylar bag sealed into a larger bag/Tupperware box with desiccant (correct)
  • In an open kit
  • In a mylar bag
  • In a Tupperware box
  • How long can the solution with protease added to the lysis buffer be stored in the fridge?

  • 3 weeks
  • 4 weeks
  • 1 week
  • 2 weeks (correct)
  • How long can diluted lysing agent 1 (24E assay) be stored at 20-30°C?

    <p>6 months</p> Signup and view all the answers

    How long can the mixture of diluted lysing agent 1 and lysing agent 2 be used?

    <p>4 hours</p> Signup and view all the answers

    How long can cooling blocks be used after removal from the fridge?

    <p>30 mins</p> Signup and view all the answers

    How long can unopened lysates be stored when refrigerated at 4°C?

    <p>7 days</p> Signup and view all the answers

    Where should sample preparation be done for the BAX Applications Project?

    <p>On the bench in the main laboratory</p> Signup and view all the answers

    What should be done when doing BAX application projects?

    <p>Run a reference detection method alongside the BAX method</p> Signup and view all the answers

    Where can guidelines for validated food products be found?

    <p>In the BAX system User Guide for each BAX kit</p> Signup and view all the answers

    What should be done to prepare bacterial cultures for MicroSnap/InSite?

    <p>Inoculate, activate and incubate MicroSnap according to kit insert</p> Signup and view all the answers

    What should be done before running BAX kits?

    <p>Reconstitute buffers according to the kit insert or User Guide</p> Signup and view all the answers

    When should PCR tubes be hydrated for accurate results?

    <p>When the instrument reaches correct temperature to load</p> Signup and view all the answers

    What should be done when running the full process of BAX kits?

    <p>Open the rack drawer by pressing the dimpled area on the right side</p> Signup and view all the answers

    Where can guidance on running a RiboPrinter assay be found?

    <p>In the RP Program User Guide</p> Signup and view all the answers

    Reagents should be stored in the freezer to extend their shelf life.

    <p>False</p> Signup and view all the answers

    The mylar bag of PCR tubes should be sealed with desiccant if storing for more than 3 weeks.

    <p>True</p> Signup and view all the answers

    The protease solution can be stored in the fridge for up to 2 weeks.

    <p>True</p> Signup and view all the answers

    Diluted lysing agent 1 (24E assay) can be stored at room temperature for up to 6 months.

    <p>False</p> Signup and view all the answers

    The mixture of diluted lysing agent 1 and lysing agent 2 must be used within 4 hours.

    <p>True</p> Signup and view all the answers

    Cooling blocks should be refrigerated and used within 30 minutes of removal from the fridge.

    <p>True</p> Signup and view all the answers

    Opened lysates can be stored for up to one week when frozen at -20°C.

    <p>True</p> Signup and view all the answers

    The BAX method should always be run without running a reference detection method alongside it.

    <p>False</p> Signup and view all the answers

    ISO guidelines for food product testing can be found in the BAX system User Guide for each BAX kit.

    <p>True</p> Signup and view all the answers

    To minimize contamination, lysis steps for BAX kits should be performed in the PCR workstation hood or biosafety cabinet.

    <p>True</p> Signup and view all the answers

    PCR tubes should be hydrated before loading them into the instrument to ensure accurate results.

    <p>False</p> Signup and view all the answers

    All standard and 24E kits can be run simultaneously in the BAX instrument.

    <p>True</p> Signup and view all the answers

    The RiboPrinter can be used to obtain an ID for cultures isolated through the reference method.

    <p>True</p> Signup and view all the answers

    The BAX results should be saved into the project folder after downloading them from the BAX computer.

    <p>True</p> Signup and view all the answers

    A report summarizing the key findings should be submitted for approval by the Applications Manager after completing the project.

    <p>True</p> Signup and view all the answers

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