Podcast
Questions and Answers
Which temperature range should reagents be kept at?
Which temperature range should reagents be kept at?
- -20°C
- 2-8°C (correct)
- 4°C
- 20-30°C
How should PCR tubes with tablets be stored for more than 3 weeks?
How should PCR tubes with tablets be stored for more than 3 weeks?
- In a mylar bag sealed into a larger bag/Tupperware box with desiccant (correct)
- In an open kit
- In a mylar bag
- In a Tupperware box
How long can the solution with protease added to the lysis buffer be stored in the fridge?
How long can the solution with protease added to the lysis buffer be stored in the fridge?
- 3 weeks
- 4 weeks
- 1 week
- 2 weeks (correct)
How long can diluted lysing agent 1 (24E assay) be stored at 20-30°C?
How long can diluted lysing agent 1 (24E assay) be stored at 20-30°C?
How long can the mixture of diluted lysing agent 1 and lysing agent 2 be used?
How long can the mixture of diluted lysing agent 1 and lysing agent 2 be used?
How long can cooling blocks be used after removal from the fridge?
How long can cooling blocks be used after removal from the fridge?
How long can unopened lysates be stored when refrigerated at 4°C?
How long can unopened lysates be stored when refrigerated at 4°C?
Where should sample preparation be done for the BAX Applications Project?
Where should sample preparation be done for the BAX Applications Project?
What should be done when doing BAX application projects?
What should be done when doing BAX application projects?
Where can guidelines for validated food products be found?
Where can guidelines for validated food products be found?
What should be done to prepare bacterial cultures for MicroSnap/InSite?
What should be done to prepare bacterial cultures for MicroSnap/InSite?
What should be done before running BAX kits?
What should be done before running BAX kits?
When should PCR tubes be hydrated for accurate results?
When should PCR tubes be hydrated for accurate results?
What should be done when running the full process of BAX kits?
What should be done when running the full process of BAX kits?
Where can guidance on running a RiboPrinter assay be found?
Where can guidance on running a RiboPrinter assay be found?
Reagents should be stored in the freezer to extend their shelf life.
Reagents should be stored in the freezer to extend their shelf life.
The mylar bag of PCR tubes should be sealed with desiccant if storing for more than 3 weeks.
The mylar bag of PCR tubes should be sealed with desiccant if storing for more than 3 weeks.
The protease solution can be stored in the fridge for up to 2 weeks.
The protease solution can be stored in the fridge for up to 2 weeks.
Diluted lysing agent 1 (24E assay) can be stored at room temperature for up to 6 months.
Diluted lysing agent 1 (24E assay) can be stored at room temperature for up to 6 months.
The mixture of diluted lysing agent 1 and lysing agent 2 must be used within 4 hours.
The mixture of diluted lysing agent 1 and lysing agent 2 must be used within 4 hours.
Cooling blocks should be refrigerated and used within 30 minutes of removal from the fridge.
Cooling blocks should be refrigerated and used within 30 minutes of removal from the fridge.
Opened lysates can be stored for up to one week when frozen at -20°C.
Opened lysates can be stored for up to one week when frozen at -20°C.
The BAX method should always be run without running a reference detection method alongside it.
The BAX method should always be run without running a reference detection method alongside it.
ISO guidelines for food product testing can be found in the BAX system User Guide for each BAX kit.
ISO guidelines for food product testing can be found in the BAX system User Guide for each BAX kit.
To minimize contamination, lysis steps for BAX kits should be performed in the PCR workstation hood or biosafety cabinet.
To minimize contamination, lysis steps for BAX kits should be performed in the PCR workstation hood or biosafety cabinet.
PCR tubes should be hydrated before loading them into the instrument to ensure accurate results.
PCR tubes should be hydrated before loading them into the instrument to ensure accurate results.
All standard and 24E kits can be run simultaneously in the BAX instrument.
All standard and 24E kits can be run simultaneously in the BAX instrument.
The RiboPrinter can be used to obtain an ID for cultures isolated through the reference method.
The RiboPrinter can be used to obtain an ID for cultures isolated through the reference method.
The BAX results should be saved into the project folder after downloading them from the BAX computer.
The BAX results should be saved into the project folder after downloading them from the BAX computer.
A report summarizing the key findings should be submitted for approval by the Applications Manager after completing the project.
A report summarizing the key findings should be submitted for approval by the Applications Manager after completing the project.
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