Microbial Growth/ Control
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Questions and Answers

Which of the following is a characteristic that effects microbial growth?

  • Chemical and physical factors (correct)
  • Continuous culture
  • Reproductive strategies
  • Binary fission

What is the purpose of a dilution series in measuring bacterial growth?

  • To identify the growth phases
  • To measure the viable count (correct)
  • To determine the lag phase
  • To estimate the cell count

In a continuous culture, what happens to the culture when fresh medium is trickled in at a steady rate?

  • The culture reaches the death phase
  • The culture enters the stationary phase
  • The culture remains in the exponential phase (correct)
  • The culture undergoes binary fission

What is the reproductive strategy of bacteria and archaea?

<p>Asexual only (A)</p> Signup and view all the answers

What is the reproductive strategy of eukaryotic microbes?

<p>Sexual and asexual (B)</p> Signup and view all the answers

What is the purpose of a chemostat or turbidostat in continuous culture?

<p>To simulate natural environments (A)</p> Signup and view all the answers

When should you inoculate the bacteria in the laboratory experiment?

<p>When the cell concentration reaches 5 x 10^8 cells/ml (A)</p> Signup and view all the answers

What must bacteria and archaea do prior to division?

<p>Replicate and segregate the genome (D)</p> Signup and view all the answers

Which phase of bacterial growth is characterized by cells dividing repeatedly at their maximal rate?

<p>Exponential phase (A)</p> Signup and view all the answers

Which method of counting bacteria is typically carried out by a dilution series and counting only plates with 30-300 colonies?

<p>Viable count (A)</p> Signup and view all the answers

Which optical technique can be used to estimate cell numbers accurately by measuring visible turbidity?

<p>Absorbance method (C)</p> Signup and view all the answers

What is the formula to calculate final number of Cells?

<p>Final cell count = number of cell originally * 2^ number of generation (B)</p> Signup and view all the answers

What is the purpose of the lag phase in bacterial growth?

<p>Regenerate pools of essential nutrients before growth (C)</p> Signup and view all the answers

What is the main reason for the death phase in bacterial growth?

<p>All of the above (D)</p> Signup and view all the answers

Which technique can be used to count cells directly and give an accurate number, but cannot distinguish between living and dead cells?

<p>Petroff-Hauser chamber (D)</p> Signup and view all the answers

In continuous culture using a chemostat, what controls the microbial growth rate?

<p>Flow rate (B)</p> Signup and view all the answers

Which of the following is NOT considered a psychrophile?

<p>An organism with an optimum temperature of 25°C (D)</p> Signup and view all the answers

Which of the following is a characteristic of thermophiles?

<p>Optimum temperature of 60°C (B)</p> Signup and view all the answers

Which of the following types of organisms can grow equally well with or without oxygen?

<p>Aerotolerant anaerobes (A)</p> Signup and view all the answers

Where are biofilms most commonly found?

<p>On conditioned surfaces (A)</p> Signup and view all the answers

What is the term for the density-dependent communication between bacterial cells in biofilms?

<p>Quorum sensing (A)</p> Signup and view all the answers

What is the autoinducer molecule produced by many gram-negative organisms in quorum sensing?

<p>AHL (C)</p> Signup and view all the answers

Which of the following is NOT a factor that may affect microbial growth?

<p>Type of substrate (D)</p> Signup and view all the answers

Which of the following is a characteristic of mesophiles?

<p>Optimum temperature of 30°C (A)</p> Signup and view all the answers

Which of the following is a characteristic of facultative anaerobes?

<p>Grow with or without oxygen, but prefer oxygen (B)</p> Signup and view all the answers

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