Mastering Immersion Methods and the Becke Line Phenomenon

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Questions and Answers

Which method is used to measure the refractive index (RI) of a particle immersed in a liquid of different refractive index?

  • Objective Distance
  • Diffraction Halo
  • Oblique Illumination
  • Becke Line (correct)

What happens to the Becke Line when the particle has a higher RI index than the medium?

  • Disappears
  • Moves toward the bright center (correct)
  • Moves away from the dark center
  • Stays in the same position

What happens to the Becke Line when the particle has a lower RI index than the medium?

  • Moves toward the bright center
  • Disappears
  • Stays in the same position
  • Moves away from the dark center (correct)

What is the purpose of Oblique Illumination in RI measurement?

<p>To create asymmetrical lighting (B)</p> Signup and view all the answers

What is the name of the contrast that outlines a transparent, irregular particle immersed in a liquid of different refractive index?

<p>Becke Line (C)</p> Signup and view all the answers

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