Mastering Conventional Blood Culture Techniques

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Questions and Answers

Which method is used to detect bacteria in conventional blood culture?

  • Visual examination of bottles
  • Sub-culturing on chocolate blood agar (correct)
  • Incubation in commercially prepared bottles
  • Detection of carbon dioxide production

What is the purpose of venting the blood culture bottle with a sterile, cotton-plugged needle?

  • To detect bacteria by acridine orange stain
  • To allow the growth of facultative organisms
  • To provide a low oxidation-reduction potential (correct)
  • To grow obligate bacteria

How can bacteria be detected after 6 to 18 hours of incubation in conventional blood culture?

  • By acridine orange stain
  • By sub-culturing on chocolate blood agar (correct)
  • By visual examination of bottles
  • By measuring carbon dioxide production

What does the BACTEC system measure to detect bacterial growth?

<p>Carbon dioxide production (B)</p> Signup and view all the answers

Which of the following is a characteristic of the BACTEC system?

<p>It is fully automated with incubator, shaker, and detector (B)</p> Signup and view all the answers

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