Mastering Aseptic Technique

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Which of the following is NOT a recommended practice for handling liquid or cultures in the lab?

Discard gloves and wash hands

Reuse tips/loops/spreaders (correct)

Always replace lids on containers

Clean up once finished

What should be included in the correct labeling of agar plates?

Organism's full name or shorthand name

The type of agar

The date of streaking

All of the above (correct)

What is the purpose of the primary bed in a streak plate?

To create a thick lawn of bacteria (correct)

To obtain single colonies

To stop the thawing of bacteria

To dilute the culture down the plate

How many streaks should be made to create the secondary bed in a streak plate?

Three Signup and view all the answers

What should be done after creating the tertiary bed in a streak plate?

Discard the loop Signup and view all the answers

What is the purpose of an overnight culture?

To provide a working liquid culture Signup and view all the answers

What should be done before pipetting the broth into a labeled bijou for an overnight culture?

Remove a single colony from the streak plate Signup and view all the answers

What should be done with the loop after transferring the colony into the filled bijou for an overnight culture?

Discard the loop Signup and view all the answers

What should be done after finishing the experiment in the lab?

All of the above Signup and view all the answers

How many cultures are permitted out of the freezer at a time?

5 Signup and view all the answers

Which location provides the most protection for staff and the most sterile environment if kept clear and clean?

Biological safety cabinet (BSC) Signup and view all the answers

What should be done before handling any equipment, reagents, or cultures in an experiment?

Clean the gloves with Klericide Signup and view all the answers

What is the aim of aseptic technique?

To prevent contamination by taking simple measures Signup and view all the answers

Which location is the least sterile but adequate for most lab work if kept clear and clean?

Benchtop Signup and view all the answers

What should be done to avoid compromising the sterility of sterile equipment before use?

Open any sterile equipment carefully Signup and view all the answers

What should be done to avoid touching anything that is not prepared and cleaned for use?

Avoid touching sterile equipment Signup and view all the answers

What should be done to avoid the need to pass hands or containers over other items being used?

Arrange equipment to avoid the need Signup and view all the answers

What is the purpose of the gloves in aseptic technique?

To protect staff from contamination Signup and view all the answers

What is the purpose of the fume hood in aseptic technique?

To protect staff from fumes Signup and view all the answers

What should be done to the chosen surface before using it in a laboratory task?

Wipe down the work area with Virkon Signup and view all the answers

Which of the following is the correct procedure for creating an overnight culture using a bead?

Using a sterile loop, take a bead out of the vial of organism of choice and gently place into the bijoux. Signup and view all the answers

What is the purpose of creating a streak plate when using a bead to create an overnight culture?

To confirm the purity of the bead. Signup and view all the answers

What is the appropriate volume to resuspend the pellet in new media during the washing of bacteria?

The same volume as the initial media. Signup and view all the answers

What is the maximum RPM for general use when using a centrifuge?

4000-5000rpm Signup and view all the answers

Which of the following is the correct procedure for washing bacteria?

Place the Eppendorfs inside their appropriate holes, ensuring they are placed opposite each other. Signup and view all the answers

What is the purpose of using immersion oil when visualizing bacteria under a microscope?

To refine the clarity of the image. Signup and view all the answers

What is the appropriate temperature for incubating the bijoux in an incubator?

37°C Signup and view all the answers

What should be done if there is not enough sample or an even number of Eppendorf tubes needed for washing bacteria?

Fill the remaining Eppendorf with an equal volume of the media used to culture the bacteria. Signup and view all the answers

What should be done after removing the supernatant in the centrifuge during the washing of bacteria?

Re-suspend the pellet in new media. Signup and view all the answers

What is the appropriate way to handle a vial when using a bead to create an overnight culture?

Open one vial at a time. Signup and view all the answers