Podcast
Questions and Answers
Which of the following is NOT a recommended practice for handling liquid or cultures in the lab?
Which of the following is NOT a recommended practice for handling liquid or cultures in the lab?
- Discard gloves and wash hands
- Reuse tips/loops/spreaders (correct)
- Always replace lids on containers
- Clean up once finished
What should be included in the correct labeling of agar plates?
What should be included in the correct labeling of agar plates?
- Organism's full name or shorthand name
- The type of agar
- The date of streaking
- All of the above (correct)
What is the purpose of the primary bed in a streak plate?
What is the purpose of the primary bed in a streak plate?
- To create a thick lawn of bacteria (correct)
- To obtain single colonies
- To stop the thawing of bacteria
- To dilute the culture down the plate
How many streaks should be made to create the secondary bed in a streak plate?
How many streaks should be made to create the secondary bed in a streak plate?
What should be done after creating the tertiary bed in a streak plate?
What should be done after creating the tertiary bed in a streak plate?
What is the purpose of an overnight culture?
What is the purpose of an overnight culture?
What should be done before pipetting the broth into a labeled bijou for an overnight culture?
What should be done before pipetting the broth into a labeled bijou for an overnight culture?
What should be done with the loop after transferring the colony into the filled bijou for an overnight culture?
What should be done with the loop after transferring the colony into the filled bijou for an overnight culture?
What should be done after finishing the experiment in the lab?
What should be done after finishing the experiment in the lab?
How many cultures are permitted out of the freezer at a time?
How many cultures are permitted out of the freezer at a time?
Which location provides the most protection for staff and the most sterile environment if kept clear and clean?
Which location provides the most protection for staff and the most sterile environment if kept clear and clean?
What should be done before handling any equipment, reagents, or cultures in an experiment?
What should be done before handling any equipment, reagents, or cultures in an experiment?
What is the aim of aseptic technique?
What is the aim of aseptic technique?
Which location is the least sterile but adequate for most lab work if kept clear and clean?
Which location is the least sterile but adequate for most lab work if kept clear and clean?
What should be done to avoid compromising the sterility of sterile equipment before use?
What should be done to avoid compromising the sterility of sterile equipment before use?
What should be done to avoid touching anything that is not prepared and cleaned for use?
What should be done to avoid touching anything that is not prepared and cleaned for use?
What should be done to avoid the need to pass hands or containers over other items being used?
What should be done to avoid the need to pass hands or containers over other items being used?
What is the purpose of the gloves in aseptic technique?
What is the purpose of the gloves in aseptic technique?
What is the purpose of the fume hood in aseptic technique?
What is the purpose of the fume hood in aseptic technique?
What should be done to the chosen surface before using it in a laboratory task?
What should be done to the chosen surface before using it in a laboratory task?
Which of the following is the correct procedure for creating an overnight culture using a bead?
Which of the following is the correct procedure for creating an overnight culture using a bead?
What is the purpose of creating a streak plate when using a bead to create an overnight culture?
What is the purpose of creating a streak plate when using a bead to create an overnight culture?
What is the appropriate volume to resuspend the pellet in new media during the washing of bacteria?
What is the appropriate volume to resuspend the pellet in new media during the washing of bacteria?
What is the maximum RPM for general use when using a centrifuge?
What is the maximum RPM for general use when using a centrifuge?
Which of the following is the correct procedure for washing bacteria?
Which of the following is the correct procedure for washing bacteria?
What is the purpose of using immersion oil when visualizing bacteria under a microscope?
What is the purpose of using immersion oil when visualizing bacteria under a microscope?
What is the appropriate temperature for incubating the bijoux in an incubator?
What is the appropriate temperature for incubating the bijoux in an incubator?
What should be done if there is not enough sample or an even number of Eppendorf tubes needed for washing bacteria?
What should be done if there is not enough sample or an even number of Eppendorf tubes needed for washing bacteria?
What should be done after removing the supernatant in the centrifuge during the washing of bacteria?
What should be done after removing the supernatant in the centrifuge during the washing of bacteria?
What is the appropriate way to handle a vial when using a bead to create an overnight culture?
What is the appropriate way to handle a vial when using a bead to create an overnight culture?
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