Tools of Molecular Genetics I,II

Play an AI-generated podcast conversation about this lesson

Which of the following is a tool used in molecular genetics for gene expression profiling?

Polymerase chain reaction

Restriction endonucleases

Microarrays (correct)

Gel electrophoresis

What is the purpose of Southern blotting?

To create genomic libraries

To analyze gene expression

To create recombinant DNA

To measure DNA fragment sizes (correct)

Which of the following is a type of cloning vector commonly used in genetic engineering?

Plasmid vectors (correct)

PCR libraries

cDNA libraries

Genomic libraries

Which type of RNA is used to construct a cDNA library?

mRNA Signup and view all the answers

What is ligated to cDNA fragments for massively parallel sequencing?

Sequencing adaptors Signup and view all the answers

What do the red boxes in Figure C represent?

Exon reads Signup and view all the answers

Which of the following is NOT a step in a single PCR cycle?

Separation of DNA strands by size Signup and view all the answers

What is the main purpose of PCR?

To amplify specific sequences from samples of genomic DNA Signup and view all the answers

Which method is commonly used for DNA sequencing?

Dideoxynucleotide (Sanger) Method Signup and view all the answers

What is the main advantage of Next Generation Sequencing (NGS) technology?

It can sequence an entire human genome in a single day Signup and view all the answers

Which enzyme is commonly used for enzymatic digestion of DNA at a specific sequence?

EcoRI Signup and view all the answers

What is the purpose of gel electrophoresis in the RNA-seq method?

To separate DNA fragments according to size Signup and view all the answers

What is the purpose of Southern blotting in the RNA-seq method?

To detect specific DNA fragments by hybridization Signup and view all the answers

Which enzyme is commonly used for DNA amplification in the polymerase chain reaction (PCR)?

Taq Polymerase Signup and view all the answers

Which of the following is true about cloning using plasmid vectors?

The DNA inserts have EcoRI-compatible ends Signup and view all the answers

What is the purpose of the cos sites in l phage cloning vectors?

To enable circularization of the vector in the host bacterium Signup and view all the answers

What is the main difference between genomic libraries and cDNA libraries?

Genomic libraries contain DNA fragments from the entire genome, while cDNA libraries only contain expressed sequences Signup and view all the answers

What is the purpose of microarrays in gene expression profiling?

To measure the expression levels of thousands of genes Signup and view all the answers