Tools of Molecular Genetics I,II
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Questions and Answers

Which of the following is a tool used in molecular genetics for gene expression profiling?

  • Polymerase chain reaction
  • Restriction endonucleases
  • Microarrays (correct)
  • Gel electrophoresis
  • What is the purpose of Southern blotting?

  • To create genomic libraries
  • To analyze gene expression
  • To create recombinant DNA
  • To measure DNA fragment sizes (correct)
  • Which of the following is a type of cloning vector commonly used in genetic engineering?

  • Plasmid vectors (correct)
  • PCR libraries
  • cDNA libraries
  • Genomic libraries
  • Which type of RNA is used to construct a cDNA library?

    <p>mRNA</p> Signup and view all the answers

    What is ligated to cDNA fragments for massively parallel sequencing?

    <p>Sequencing adaptors</p> Signup and view all the answers

    What do the red boxes in Figure C represent?

    <p>Exon reads</p> Signup and view all the answers

    Which of the following is NOT a step in a single PCR cycle?

    <p>Separation of DNA strands by size</p> Signup and view all the answers

    What is the main purpose of PCR?

    <p>To amplify specific sequences from samples of genomic DNA</p> Signup and view all the answers

    Which method is commonly used for DNA sequencing?

    <p>Dideoxynucleotide (Sanger) Method</p> Signup and view all the answers

    What is the main advantage of Next Generation Sequencing (NGS) technology?

    <p>It can sequence an entire human genome in a single day</p> Signup and view all the answers

    Which enzyme is commonly used for enzymatic digestion of DNA at a specific sequence?

    <p>EcoRI</p> Signup and view all the answers

    What is the purpose of gel electrophoresis in the RNA-seq method?

    <p>To separate DNA fragments according to size</p> Signup and view all the answers

    What is the purpose of Southern blotting in the RNA-seq method?

    <p>To detect specific DNA fragments by hybridization</p> Signup and view all the answers

    Which enzyme is commonly used for DNA amplification in the polymerase chain reaction (PCR)?

    <p>Taq Polymerase</p> Signup and view all the answers

    Which of the following is true about cloning using plasmid vectors?

    <p>The DNA inserts have EcoRI-compatible ends</p> Signup and view all the answers

    What is the purpose of the cos sites in l phage cloning vectors?

    <p>To enable circularization of the vector in the host bacterium</p> Signup and view all the answers

    What is the main difference between genomic libraries and cDNA libraries?

    <p>Genomic libraries contain DNA fragments from the entire genome, while cDNA libraries only contain expressed sequences</p> Signup and view all the answers

    What is the purpose of microarrays in gene expression profiling?

    <p>To measure the expression levels of thousands of genes</p> Signup and view all the answers

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