Podcast
Questions and Answers
Which of the following is a tool used in molecular genetics for gene expression profiling?
Which of the following is a tool used in molecular genetics for gene expression profiling?
- Polymerase chain reaction
- Restriction endonucleases
- Microarrays (correct)
- Gel electrophoresis
What is the purpose of Southern blotting?
What is the purpose of Southern blotting?
- To create genomic libraries
- To analyze gene expression
- To create recombinant DNA
- To measure DNA fragment sizes (correct)
Which of the following is a type of cloning vector commonly used in genetic engineering?
Which of the following is a type of cloning vector commonly used in genetic engineering?
- Plasmid vectors (correct)
- PCR libraries
- cDNA libraries
- Genomic libraries
Which type of RNA is used to construct a cDNA library?
Which type of RNA is used to construct a cDNA library?
What is ligated to cDNA fragments for massively parallel sequencing?
What is ligated to cDNA fragments for massively parallel sequencing?
What do the red boxes in Figure C represent?
What do the red boxes in Figure C represent?
Which of the following is NOT a step in a single PCR cycle?
Which of the following is NOT a step in a single PCR cycle?
What is the main purpose of PCR?
What is the main purpose of PCR?
Which method is commonly used for DNA sequencing?
Which method is commonly used for DNA sequencing?
What is the main advantage of Next Generation Sequencing (NGS) technology?
What is the main advantage of Next Generation Sequencing (NGS) technology?
Which enzyme is commonly used for enzymatic digestion of DNA at a specific sequence?
Which enzyme is commonly used for enzymatic digestion of DNA at a specific sequence?
What is the purpose of gel electrophoresis in the RNA-seq method?
What is the purpose of gel electrophoresis in the RNA-seq method?
What is the purpose of Southern blotting in the RNA-seq method?
What is the purpose of Southern blotting in the RNA-seq method?
Which enzyme is commonly used for DNA amplification in the polymerase chain reaction (PCR)?
Which enzyme is commonly used for DNA amplification in the polymerase chain reaction (PCR)?
Which of the following is true about cloning using plasmid vectors?
Which of the following is true about cloning using plasmid vectors?
What is the purpose of the cos sites in l phage cloning vectors?
What is the purpose of the cos sites in l phage cloning vectors?
What is the main difference between genomic libraries and cDNA libraries?
What is the main difference between genomic libraries and cDNA libraries?
What is the purpose of microarrays in gene expression profiling?
What is the purpose of microarrays in gene expression profiling?
