Mass Spectrometry Principles

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Questions and Answers

What is the primary function of a mass spectrometer?

  • To analyze the structure of a gas phase molecule
  • To detect the relative abundance of ionic species
  • To generate charged molecules or fragments (correct)
  • To separate ions based on their mass

What happens to atoms when they become ions?

  • They lose or gain protons
  • They lose or gain neutrons
  • They lose or gain molecules
  • They lose or gain electrons (correct)

What is the basis for separating ions in a mass spectrometer?

  • Their atomic number
  • Their charge alone
  • Their mass alone
  • Their mass-to-charge ratio (correct)

What is recorded or measured at the end of the mass spectrometry process?

<p>The relative abundance of each ionic species (D)</p> Signup and view all the answers

What is the term for a group of electrically charged atoms?

<p>Ion (D)</p> Signup and view all the answers

What is the primary goal of ionizing a gas phase molecule in a mass spectrometer?

<p>To analyze the masses of the produced ions (B)</p> Signup and view all the answers

What is the result of an atom losing or gaining electrons?

<p>The formation of an electrically charged ion (C)</p> Signup and view all the answers

What is the relationship between the mass and charge of an ion in a mass spectrometer?

<p>The mass-to-charge ratio is used to separate ions (D)</p> Signup and view all the answers

What is the final step in the mass spectrometry process?

<p>Recording the relative abundance of each ionic species (A)</p> Signup and view all the answers

What is an ionic species?

<p>A type of ion with a specific mass-to-charge ratio (B)</p> Signup and view all the answers

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Study Notes

Mass Spectrometry

  • A mass spectrometer generates charged molecules or fragments of an analyte in a gas phase.
  • The objective of a mass spectrometer is to ionize a gas phase molecule and analyze the masses of its produced ions.

Ionization

  • Ions are electrically charged atoms or groups of atoms formed when atoms lose or gain electrons.
  • Atoms can become negatively charged (gain electrons) or positively charged (lose electrons) ions.

Separation and Detection

  • Produced ions are separated according to their mass-to-charge ratio (m/z).
  • The relative abundance of each ionic species present is recorded (measured) after separation.

Mass Spectrometry

  • A mass spectrometer generates charged molecules or fragments of an analyte in a gas phase.
  • The objective of a mass spectrometer is to ionize a gas phase molecule and analyze the masses of its produced ions.

Ionization

  • Ions are electrically charged atoms or groups of atoms formed when atoms lose or gain electrons.
  • Atoms can become negatively charged (gain electrons) or positively charged (lose electrons) ions.

Separation and Detection

  • Produced ions are separated according to their mass-to-charge ratio (m/z).
  • The relative abundance of each ionic species present is recorded (measured) after separation.

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