Mass Spectrometry Principles Revision Lecture
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Questions and Answers

What ionization method is suitable for many non-polar compounds?

  • Electrospray ionization (ESI)
  • Protonation
  • Atmospheric pressure photoionization (APPI) (correct)
  • Photoionization

Which ionization method produces radical ions and protonated/deprotonated species?

  • Electrospray ionization (ESI)
  • Protonation
  • Deprotonation
  • Photoionization (correct)

What does the spacing between isotopologues determine in mass spectrometry?

  • Molecular weight
  • Charge state (correct)
  • Resolving power
  • Neutral molecule mass

Which parameter tells us about the charge state in mass spectrometry?

<p>Isotopologue spacing (D)</p> Signup and view all the answers

In electrospray ionization (ESI), what is measured on the mass spectrometer?

<p>$M + nH+$ (D)</p> Signup and view all the answers

What should the resolving power have to be in order to resolve isotopologues in mass spectrometry?

<p>High (C)</p> Signup and view all the answers

Which course should students search for if they are looking for past papers from the year 2021?

<p>CH3G5 (B)</p> Signup and view all the answers

Which topic was included in the older course but is not part of CH3F2 from 2019-2020 onwards?

<p>Interpretation of EI mass spectra (C)</p> Signup and view all the answers

In which book can students find information on Mass Spectrometry?

<p>Mass Spectrometry: Principles and Applications by Edmond de Hoffmann and Vincent Stroobant (C)</p> Signup and view all the answers

Which author did not write a book on Mass Spectrometry?

<p>Laura Brown (D)</p> Signup and view all the answers

Where can students find past papers for the year 2020-2021?

<p>Moodle platform (C)</p> Signup and view all the answers

What is the correct formula for the neutral form of naphthalene?

<p>C10H8 (C)</p> Signup and view all the answers

In positive-ion mode for naphthalene by APPI, what is the correct representation for the singly-charged species?

<p>C10H8 + H (C)</p> Signup and view all the answers

What is the charge on an electron?

<p>1.60 x 10^-19 C (B)</p> Signup and view all the answers

What is wrong with the calculation that was performed for the neutral form of C10H8?

<p>Used average masses instead of monoisotopic masses (A)</p> Signup and view all the answers

Which unit should be used for reporting masses instead of 'amu'?

<p>Da (C)</p> Signup and view all the answers

What number of significant figures should have been used in the calculation?

<p>~5 (B)</p> Signup and view all the answers

Why is MALDI ionization well-suited for polymer analysis?

<p>It avoids the formation of multiply-charged ions. (C)</p> Signup and view all the answers

In MALDI-TOF, what is the role of the TOF mass analyzer?

<p>It provides resolving power for the experiments. (D)</p> Signup and view all the answers

How is the n value calculated in the given equations?

<p>n = (b - 1.007) / (a - b) (D)</p> Signup and view all the answers

What experimental advantages does TOF offer in MALDI-TOF analysis?

<p>High speed of data acquisition (C)</p> Signup and view all the answers

How does the combination of MALDI and TOF overcome data analysis complexities in polymer analysis?

<p>By avoiding overlaps of polymer distributions. (D)</p> Signup and view all the answers

What is the purpose of choosing an organic matrix in MALDI ionization technique?

<p>To minimize the fragmentation of the analyte (C)</p> Signup and view all the answers

In MALDI, what is the typical ratio of matrix to analyte in the solution prepared for deposition?

<p>1000:1 (A)</p> Signup and view all the answers

What is the role of the UV laser in MALDI ionization?

<p>To facilitate ionization of the analyte molecules (B)</p> Signup and view all the answers

Which ionization mode occurs when the analyte is protonated in MALDI?

<p>Positive-ion mode (C)</p> Signup and view all the answers

What is a common observation in APPI mechanisms according to the text?

<p>Aliphatic compounds show loss of CO2 and HCOOH in APPI (A)</p> Signup and view all the answers

What nomenclature was proposed by Roepstorff and Fohlman regarding sequence ions in mass spectra of peptides?

<p>Roepstorff cleavage nomenclature (C)</p> Signup and view all the answers

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