Marine Microbiology Molecular Techniques Quiz

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Questions and Answers

What is the main difference between Gram-positive and Gram-negative bacteria?

  • Presence of a thick peptidoglycan layer in Gram-positive bacteria.
  • Differences in the structure of the cell wall. (correct)
  • Presence of an outer membrane in Gram-negative bacteria.
  • Method of staining used during observation.

What does VBNC (Viable but Non-Cultivable) refer to in marine microbiology?

  • Bacteria that are easily observable under a microscope.
  • Bacteria that are resistant to staining methods.
  • Bacteria that exist but cannot be cultured using standard techniques. (correct)
  • Bacteria that can only grow on specific culture media.

What do molecular methods involve in marine microbiology?

  • Direct observation of bacteria under a microscope.
  • Analysis of DNA, RNA, and proteins to describe microbial communities. (correct)
  • Staining of bacterial samples for identification purposes.
  • Culturing bacteria on different media to observe growth patterns.

Why is RNA better suited for studying the function of a type of bacteria compared to DNA?

<p>RNA can make copies until it produces the required protein function. (C)</p> Signup and view all the answers

What is the main function of Taq polymerase in PCR (Polymerase Chain Reaction)?

<p>Making copies of DNA segments during the PCR process. (C)</p> Signup and view all the answers

Why is the 16S ribosomal RNA gene often used as a gene marker in bacterial studies?

<p>It has high variability across different bacterial species. (B)</p> Signup and view all the answers

What is the target of bacterial gene identification and why does it have high variability?

<p>Single species identification (D)</p> Signup and view all the answers

What is the difference between environmental DNA (eDNA) and general DNA?

<p>General DNA is isolated from the environment, while eDNA is DNA from inside the cell (A)</p> Signup and view all the answers

What is the impact of molecular biology on marine microbiology?

<p>Insight into microbial ecology and physiology (B)</p> Signup and view all the answers

What does 'omic' sciences involve?

<p>Exploring DNA and RNA to understand bacterial functions (B)</p> Signup and view all the answers

Why are microbes important for the food web?

<p>They help in waste decomposition (B)</p> Signup and view all the answers

How can microbes help reduce oil pollution in the ocean?

<p>By feeding on oil particles (A)</p> Signup and view all the answers

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