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Marine Microbiology Molecular Techniques Quiz

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Marine Microbiology Molecular Techniques Quiz

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Questions and Answers

What is the main difference between Gram-positive and Gram-negative bacteria?

Presence of a thick peptidoglycan layer in Gram-positive bacteria.

Differences in the structure of the cell wall. (correct)

Presence of an outer membrane in Gram-negative bacteria.

Method of staining used during observation.

What does VBNC (Viable but Non-Cultivable) refer to in marine microbiology?

Bacteria that are easily observable under a microscope.

Bacteria that are resistant to staining methods.

Bacteria that exist but cannot be cultured using standard techniques. (correct)

Bacteria that can only grow on specific culture media.

What do molecular methods involve in marine microbiology?

Direct observation of bacteria under a microscope.

Analysis of DNA, RNA, and proteins to describe microbial communities. (correct)

Staining of bacterial samples for identification purposes.

Culturing bacteria on different media to observe growth patterns.

Why is RNA better suited for studying the function of a type of bacteria compared to DNA?

RNA can make copies until it produces the required protein function. Signup and view all the answers

What is the main function of Taq polymerase in PCR (Polymerase Chain Reaction)?

Making copies of DNA segments during the PCR process. Signup and view all the answers

Why is the 16S ribosomal RNA gene often used as a gene marker in bacterial studies?

It has high variability across different bacterial species. Signup and view all the answers

What is the target of bacterial gene identification and why does it have high variability?

Single species identification Signup and view all the answers

What is the difference between environmental DNA (eDNA) and general DNA?

General DNA is isolated from the environment, while eDNA is DNA from inside the cell Signup and view all the answers

What is the impact of molecular biology on marine microbiology?

Insight into microbial ecology and physiology Signup and view all the answers

What does 'omic' sciences involve?

Exploring DNA and RNA to understand bacterial functions Signup and view all the answers

Why are microbes important for the food web?

They help in waste decomposition Signup and view all the answers

How can microbes help reduce oil pollution in the ocean?

By feeding on oil particles Signup and view all the answers