30 Questions
Which of the following organisms do not contain DNA?
Human body
What is the function of DNA in a cell?
To create proteins
Which section of the course provides a detailed explanation of DNA and proteins?
Biology of Biotech course
What is the topic of section 3 of the course?
Creating recombinant proteins
Which molecule is the building block of DNA?
Nucleotide
How many different bases are there in DNA?
Four
What is the order of base pairing in DNA?
A-T, C-G
What is the role of post-translational modifications in proteins?
To determine the shape and function of proteins
Which enzyme is used to create a gap in the plasmid and cut the human DNA at the same sequence?
EcoR1
What is the purpose of adding DNA ligase to the solution?
To seal the plasmid
What is the term used to describe DNA molecules that have been created by combining DNA from more than one source?
Recombinant DNA
Which cell types are commonly used to create recombinant proteins?
Bacterial cells and mammalian cells
Which type of cells are more sensitive to changes in the cell culture environment?
Mammalian cells
Why are bacterial cells unable to be used to create many proteins?
Bacterial cells lack the ability to perform complex protein processing
Which protein type requires the use of mammalian cells to be manufactured?
Monoclonal antibodies
What is the purpose of injecting ADVATE in patients with Hemophilia A?
To replace a missing protein
Which of the following best describes recombinant DNA?
DNA molecules that have been created by combining DNA from more than one source
What is the purpose of restriction enzymes in genetic engineering?
To recognize specific DNA sequences and cut DNA at those spots
What is a plasmid?
A circular piece of DNA that can easily be transferred between cells
How is the gene of interest integrated into a plasmid?
By cutting the plasmid with restriction enzymes and then reconnecting it with the gene of interest
Which method is used to remove bacterial cells that do not contain the plasmid?
Antibiotic resistance gene
Why is a liposome used to transfer recombinant DNA into mammalian cells?
Liposomes can easily enter the cell through the membrane
Why can't antibiotic selection be used to select mammalian cells that have taken up the plasmid?
Mammalian cells are resistant to antibiotics
Why is bacterial cell culture faster and cheaper compared to mammalian cell culture?
Bacterial cells divide more quickly than mammalian cells
Which of the following best describes fusion proteins?
Proteins that combine the characteristics of two different proteins
What is the advantage of fusion proteins like Alprolix and Eloctate?
They combine the blood clotting ability of a clotting factor with the stability of an antibody
Why are antibodies considered stable in a patient's body?
They typically last for several weeks
What is the main method used to produce most biologic drugs?
Bacterial cells or CHO cells
What is the purpose of genetically engineering animals to produce biologic drugs?
To get an animal to produce a therapeutic protein
Why are blood clotting factors not stable in a patient's body?
They need to be infused every other day
N/A
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