Introduction to Genetic Engineering
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Questions and Answers

Which of the following organisms do not contain DNA?

  • Plants
  • Bacteria
  • Yeast
  • Human body (correct)

What is the function of DNA in a cell?

  • To convert genes
  • To remind of DNA structure
  • To illustrate cell machinery
  • To create proteins (correct)

Which section of the course provides a detailed explanation of DNA and proteins?

  • Biology of Biotech course (correct)
  • Section 2
  • Section 1
  • Section 3

What is the topic of section 3 of the course?

<p>Creating recombinant proteins (C)</p> Signup and view all the answers

Which molecule is the building block of DNA?

<p>Nucleotide (C)</p> Signup and view all the answers

How many different bases are there in DNA?

<p>Four (A)</p> Signup and view all the answers

What is the order of base pairing in DNA?

<p>A-T, C-G (A)</p> Signup and view all the answers

What is the role of post-translational modifications in proteins?

<p>To determine the shape and function of proteins (A)</p> Signup and view all the answers

Which enzyme is used to create a gap in the plasmid and cut the human DNA at the same sequence?

<p>EcoR1 (D)</p> Signup and view all the answers

What is the purpose of adding DNA ligase to the solution?

<p>To seal the plasmid (D)</p> Signup and view all the answers

What is the term used to describe DNA molecules that have been created by combining DNA from more than one source?

<p>Recombinant DNA (D)</p> Signup and view all the answers

Which cell types are commonly used to create recombinant proteins?

<p>Bacterial cells and mammalian cells (B)</p> Signup and view all the answers

Which type of cells are more sensitive to changes in the cell culture environment?

<p>Mammalian cells (A)</p> Signup and view all the answers

Why are bacterial cells unable to be used to create many proteins?

<p>Bacterial cells lack the ability to perform complex protein processing (C)</p> Signup and view all the answers

Which protein type requires the use of mammalian cells to be manufactured?

<p>Monoclonal antibodies (B)</p> Signup and view all the answers

What is the purpose of injecting ADVATE in patients with Hemophilia A?

<p>To replace a missing protein (B)</p> Signup and view all the answers

Which of the following best describes recombinant DNA?

<p>DNA molecules that have been created by combining DNA from more than one source (A)</p> Signup and view all the answers

What is the purpose of restriction enzymes in genetic engineering?

<p>To recognize specific DNA sequences and cut DNA at those spots (C)</p> Signup and view all the answers

What is a plasmid?

<p>A circular piece of DNA that can easily be transferred between cells (D)</p> Signup and view all the answers

How is the gene of interest integrated into a plasmid?

<p>By cutting the plasmid with restriction enzymes and then reconnecting it with the gene of interest (A)</p> Signup and view all the answers

Which method is used to remove bacterial cells that do not contain the plasmid?

<p>Antibiotic resistance gene (B)</p> Signup and view all the answers

Why is a liposome used to transfer recombinant DNA into mammalian cells?

<p>Liposomes can easily enter the cell through the membrane (B)</p> Signup and view all the answers

Why can't antibiotic selection be used to select mammalian cells that have taken up the plasmid?

<p>Mammalian cells are resistant to antibiotics (B)</p> Signup and view all the answers

Why is bacterial cell culture faster and cheaper compared to mammalian cell culture?

<p>Bacterial cells divide more quickly than mammalian cells (B)</p> Signup and view all the answers

Which of the following best describes fusion proteins?

<p>Proteins that combine the characteristics of two different proteins (D)</p> Signup and view all the answers

What is the advantage of fusion proteins like Alprolix and Eloctate?

<p>They combine the blood clotting ability of a clotting factor with the stability of an antibody (B)</p> Signup and view all the answers

Why are antibodies considered stable in a patient's body?

<p>They typically last for several weeks (C)</p> Signup and view all the answers

What is the main method used to produce most biologic drugs?

<p>Bacterial cells or CHO cells (C)</p> Signup and view all the answers

What is the purpose of genetically engineering animals to produce biologic drugs?

<p>To get an animal to produce a therapeutic protein (C)</p> Signup and view all the answers

Why are blood clotting factors not stable in a patient's body?

<p>They need to be infused every other day (C)</p> Signup and view all the answers

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