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Questions and Answers

What is the end product of the Gelatin test?

  • Liquid amino acid + smaller peptides (correct)
  • Blue strip
  • Solid gelatin
  • Amino acids + larger peptides

    • What is the medium used in the Gelatin test?

    Gelatin strip

    What indicates a positive result for the Gelatin test?

    Blue strip or liquid gelatin

    What is the medium used in the Anaerobic Jar?

    <p>Nutrient agar</p> Signup and view all the answers

    What is the substrate in the Anaerobic Jar?

    <p>Oxygen</p> Signup and view all the answers

    What is the chemical used in the Anaerobic Jar?

    <p>Anaerogen chemical</p> Signup and view all the answers

    What is the O2 indicator in the Anaerobic Jar?

    <p>Resazorin</p> Signup and view all the answers

    What is the end product of the Anaerobic Jar?

    <p>Water</p> Signup and view all the answers

    What indicates a positive result for the Anaerobic Jar?

    <p>Clear color means that the oxygen was not present</p> Signup and view all the answers

    What indicates a positive result for the Anaerobic Jar?

    <p>Pink means that the oxygen was present</p> Signup and view all the answers

    What is the substrate in the Catalase test?

    <p>H2O2 (Hydrogen peroxide)</p> Signup and view all the answers

    What is the bacterial enzyme in the Catalase test?

    <p>Catalase Endoenzyme</p> Signup and view all the answers

    What are the end products of the Catalase test?

    <p>Water + Oxygen molecules</p> Signup and view all the answers

    Name the bacteria participating in the Catalase test.

    <p>Staphylococcus epidermidis and Enterococcus faecalis</p> Signup and view all the answers

    What is the substrate in the Oxidase test?

    <p>O2</p> Signup and view all the answers

    What is the enzyme in the Oxidase test?

    <p>Oxidase Enzyme</p> Signup and view all the answers

    What are the end products of the Oxidase test?

    <p>H2O (water)</p> Signup and view all the answers

    What indicates a positive result in the Oxidase test?

    <p>Pink</p> Signup and view all the answers

    What indicates a negative result in the Oxidase test?

    <p>Clear</p> Signup and view all the answers

    What is the medium used in the Decarboxylase test?

    <p>Decarboxylase medium</p> Signup and view all the answers

    What are the substrates in the Decarboxylase test?

    <p>Amino acids: Lysine and Ornithine</p> Signup and view all the answers

    What are the bacterial enzymes in the Decarboxylase test?

    <p>Lysine decarboxylase and Ornithine decarboxylase</p> Signup and view all the answers

    What is the end product for Lysine in the Decarboxylase test?

    <p>Cadaverine</p> Signup and view all the answers

    What is the end product for Ornithine in the Decarboxylase test?

    <p>Putrescine</p> Signup and view all the answers

    What is the appearance of a positive result in the Decarboxylase test?

    <p>Purple</p> Signup and view all the answers

    What is the appearance of a negative result in the Decarboxylase test?

    <p>Yellow</p> Signup and view all the answers

    What is the pH indicator in the Decarboxylase test?

    <p>Bromocresol purple</p> Signup and view all the answers

    What is the medium used in the Phenylalanine Deaminase test?

    <p>Phenylalanine Agar</p> Signup and view all the answers

    What is the substrate in the Phenylalanine Deaminase test?

    <p>Phenylalanine</p> Signup and view all the answers

    What is the bacterial enzyme in the Phenylalanine Deaminase test?

    <p>Phenylalanine deaminase</p> Signup and view all the answers

    What are the end products of the Phenylalanine Deaminase test?

    <p>Phenylpyruvic acid and Ammonia (NH3)</p> Signup and view all the answers

    What indicates a positive appearance in the Phenylalanine Deaminase test?

    <p>Green</p> Signup and view all the answers

    What indicates a negative result in the Phenylalanine Deaminase test?

    <p>Yellow</p> Signup and view all the answers

    What is the reagent in the Phenylalanine Deaminase test?

    <p>FeCl3 (Iron(III) chloride)</p> Signup and view all the answers

    What is the substrate in the Casein Hydrolysis test?

    <p>White casein (milk protein)</p> Signup and view all the answers

    What is the enzyme in the Casein Hydrolysis test?

    <p>Casease exoenzyme</p> Signup and view all the answers

    What are the end products of the Casein Hydrolysis test?

    <p>Amino acids, dipeptides or polypeptides</p> Signup and view all the answers

    What is the appearance of a positive result in the Casein Hydrolysis test?

    <p>Clear</p> Signup and view all the answers

    What is the appearance of a negative result in the Casein Hydrolysis test?

    <p>No change in creamy color</p> Signup and view all the answers

    What is the medium in the Urea tablet test?

    <p>Urea tablet</p> Signup and view all the answers

    What is the substrate in the Urea tablet test?

    <p>Urea</p> Signup and view all the answers

    What is the enzyme in the Urea tablet test?

    <p>Endoenzyme Urease</p> Signup and view all the answers

    What are the end products in the Urea tablet test?

    <p>2 ammonia (2NH3) and Carbon dioxide (CO2)</p> Signup and view all the answers

    What indicates a positive result in the Urea tablet test?

    <p>Hot Pink</p> Signup and view all the answers

    What indicates a negative result in the Urea tablet test?

    <p>No change; stays Yellow</p> Signup and view all the answers

    What is the pH indicator in the Urea tablet test?

    <p>Phenol Red</p> Signup and view all the answers

    Name the bacteria causing the Urea tablet test.

    <p>Proteus mirabilis</p> Signup and view all the answers

    Study Notes

    Gelatin Test

    • End product consists of liquid amino acids and smaller peptides.
    • The medium used for the test is a gelatin strip.
    • A positive result is indicated by a blue strip or liquid gelatin.

    Anaerobic Jar

    • The medium utilized is nutrient agar.
    • Oxygen serves as the substrate in the Anaerobic Jar.
    • The chemical used in this setup is anaerogen.
    • Resazorin acts as the O2 indicator, revealing oxygen presence.
    • The end product of the process is water.
    • A clear color indicates a positive result, meaning oxygen was not present.
    • A pink color suggests the presence of oxygen.

    Catalase Test

    • The substrate, which is also the reagent, is hydrogen peroxide (H2O2).
    • The bacterial enzyme involved is catalase endoenzyme.
    • The end products of this reaction are water and oxygen molecules.
    • Bacteria participating in the test include Staphylococcus epidermidis found on the skin and Enterococcus faecalis found in the gut.

    Oxidase Test

    • O2 is the substrate in the oxidase test.
    • The enzyme responsible for the reaction is the oxidase enzyme.
    • The end product of the reaction is water (H2O).
    • A pink color indicates a positive result, while a clear color denotes a negative result.

    Decarboxylase Test

    • The medium used is decarboxylase medium.
    • Amino acids lysine and ornithine act as substrates for the test.
    • Bacterial enzymes involved include lysine decarboxylase and ornithine decarboxylase.
    • The end product for lysine is cadaverine, while for ornithine, it is putrescine.
    • A positive result is indicated by a purple color, while a yellow color indicates a negative result.
    • Bromocresol purple is the pH indicator used in the test.

    Phenylalanine Deaminase Test

    • The medium used is phenylalanine agar.
    • The substrate for the test is phenylalanine.
    • The bacterial enzyme responsible is phenylalanine deaminase.
    • End products include phenylpyruvic acid and ammonia (NH3), neutralizing each other.
    • A positive appearance is indicated by a green color, while yellow signifies a negative result.
    • Iron(III) chloride (FeCl3) is the reagent used in the test.

    Casein Hydrolysis

    • The substrate is white casein, which is a milk protein.
    • The enzyme involved is casease, an exoenzyme.
    • End products include amino acids, dipeptides, or polypeptides.
    • A positive result is indicated by a clear appearance, while no change in creamy color denotes a negative result.

    Urea Tablet

    • The medium used is a urea tablet.
    • The substrate for the reaction is urea.
    • The enzyme present is urease, an endoenzyme.
    • The reaction yields two ammonia (2NH3) and carbon dioxide (CO2) as end products.
    • A positive result is indicated by a hot pink color, while a negative result remains yellow, indicating no change.
    • Phenol red functions as the pH indicator in this test.
    • Proteus mirabilis is a bacteria known to cause the urea reaction.

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