Bacteriology

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Questions and Answers

Which staining technique is used to differentiate Gram-positive and Gram-negative bacteria?

  • Capsule staining
  • Gram staining (correct)
  • Spore staining
  • Flagellar staining

What color do Gram-positive bacteria stain in Gram staining?

  • Pink
  • Red
  • Purple
  • Blue (correct)

What color do Gram-negative bacteria stain in Gram staining?

  • Pink (correct)
  • Red
  • Blue
  • Purple

Which bacterial structure can be revealed using special/structural staining techniques?

<p>Capsules (D)</p> Signup and view all the answers

Which type of bacteria are rod-shaped?

<p>Bacilli (D)</p> Signup and view all the answers

Which type of bacteria are oval-shaped and can appear as coccobacilli?

<p>Coccobacilli (C)</p> Signup and view all the answers

Which bacteria strain is found in Yakult?

<p>Lactobacilli shirota (C)</p> Signup and view all the answers

Which staining technique might have been used for the purple stain in Figure 27?

<p>Another staining technique (B)</p> Signup and view all the answers

Which bacteria are Gram-negative coccobacilli according to Figure 27?

<p>Haemophilus (A)</p> Signup and view all the answers

What is the purpose of differential staining?

<p>To differentiate bacterial structures (A)</p> Signup and view all the answers

Which staining technique is commonly used to differentiate Gram-positive and Gram-negative bacteria?

<p>Gram staining (B)</p> Signup and view all the answers

What color do Gram-positive bacteria stain in Gram staining?

<p>Blue (B)</p> Signup and view all the answers

Which bacterial structure can be revealed using special/structural staining techniques?

<p>Spores (B)</p> Signup and view all the answers

Which type of bacteria are oval-shaped and can appear as coccobacilli?

<p>Haemophilus (B)</p> Signup and view all the answers

Which bacteria strain is found in Yakult?

<p>Lactobacilli (A)</p> Signup and view all the answers

Which of the following is NOT a characteristic of bacteria that contribute to their ability to cause disease?

<p>Lack of specific receptors on host cells (D)</p> Signup and view all the answers

Which of the following enzymes is responsible for degrading collagen, the major protein of fibrous connective tissue?

<p>Collagenase (B)</p> Signup and view all the answers

Which of the following antiphagocytic factors is NOT correctly matched with its example?

<p>Pili - S. pyogenes (A)</p> Signup and view all the answers

Which of the following statements about bacterial pathogenesis is NOT true?

<p>Bacteria always cause symptoms by infecting host cells. (C)</p> Signup and view all the answers

Which of the following factors does NOT influence the degree of disease caused by bacteria?

<p>Presence of extrachromosomal genes (C)</p> Signup and view all the answers

Which of the following is NOT a natural defense mechanism of the human body against bacterial invasion?

<p>Airways (B)</p> Signup and view all the answers

Which of the following is NOT a way in which bacteria can adapt and better survive in their environment?

<p>Increased susceptibility to the immune system (C)</p> Signup and view all the answers

Which of the following is an example of a bacterial enzyme that dissolves coagulated plasma and aids in the rapid spread through tissues?

<p>Streptokinase/fibrinolysin (B)</p> Signup and view all the answers

Which of the following is NOT an example of an antiphagocytic factor produced by bacteria?

<p>M protein (A)</p> Signup and view all the answers

Which of the following is NOT a characteristic of bacterial biofilms?

<p>Ability to invade host cells (C)</p> Signup and view all the answers

Which of the following is NOT a way in which bacteria can adapt and better survive in their environment?

<p>Gaining or losing genetic traits (C)</p> Signup and view all the answers

Which of the following is NOT an example of an antiphagocytic factor produced by bacteria?

<p>Surface protein A (C)</p> Signup and view all the answers

Which of the following enzymes is responsible for degrading collagen, the major protein of fibrous connective tissue?

<p>Collagenase (D)</p> Signup and view all the answers

Which of the following is NOT a characteristic of bacterial biofilms?

<p>A slimy coat on solid surfaces (C)</p> Signup and view all the answers

Which of the following statements about bacterial pathogenesis is NOT true?

<p>Virulent bacteria always cause excessive inflammatory or immune system response (C)</p> Signup and view all the answers

Flashcards

Gram staining

A technique to differentiate Gram-positive and Gram-negative bacteria.

Gram-positive bacteria

Bacteria that stain blue in Gram staining.

Gram-negative bacteria

Bacteria that stain pink in Gram staining.

Capsules

A bacterial structure that can be revealed using special staining techniques.

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Bacilli

Rod-shaped bacteria.

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Coccobacilli

Oval-shaped bacteria that can appear as short rods.

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Lactobacilli shirota

A strain of bacteria found in Yakult.

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Differential staining

A method used to differentiate bacterial structures.

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Spores

A bacterial structure that can also be revealed with special staining.

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Haemophilus

Gram-negative coccobacilli bacteria.

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Collagenase

An enzyme that degrades collagen, major protein in connective tissue.

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Antiphagocytic factors

Traits that help bacteria avoid being engulfed by immune cells.

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Bacterial pathogenesis

The process by which bacteria cause disease.

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Bacterial biofilms

Community of bacteria adhering to surfaces, often resistant to treatment.

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Immunosuppressive adaptations

Ways bacteria can evolve to better survive the immune system.

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Virulent bacteria

Bacteria that can cause severe disease symptoms.

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Streptokinase

An enzyme that dissolves coagulated plasma to help bacteria spread.

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Airways (defense mechanism)

NOT a natural defense against bacterial invasion.

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Surface protein A

NOT an example of an antiphagocytic factor produced by bacteria.

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Pili

Hair-like structures on bacteria that aid in attachment.

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Excessive immune response

NOT a requirement for bacteria to cause symptoms.

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Lack of specific receptors

NOT a characteristic that contributes to bacterial disease ability.

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Gaining genetic traits

NOT a way for bacteria to adapt better in their environment.

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Collagen degradation

The breakdown of collagen by enzymes like collagenase.

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Inflammatory response

The body's response that can occur during bacterial infection.

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Nutrient acquisition

How bacteria obtain necessary nutrients from their environment.

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Study Notes

Bacterial Staining Techniques

  • Gram Staining is employed to distinguish between Gram-positive and Gram-negative bacteria.
  • Color Results:
    • Gram-positive bacteria stain purple due to a thick peptidoglycan layer in their cell walls.
    • Gram-negative bacteria stain pink because of a thinner peptidoglycan layer surrounded by an outer membrane.

Special/Structural Staining Techniques

  • Special or structural staining can reveal bacterial structures like capsules, endospores, and flagella.

Bacterial Shapes

  • Rod-shaped bacteria are termed bacilli.
  • Oval-shaped bacteria can appear as coccobacilli.

Specific Bacteria

  • The strain found in Yakult is Lactobacillus casei.

Differential Staining Purpose

  • Differential staining techniques serve to distinguish different types of bacteria based on their cell wall composition and structure.

Bacterial Pathogenesis and Characteristics

  • Enzyme for Collagen Degradation: Enzymes such as collagenase enable bacteria to break down collagen, facilitating tissue invasion.
  • Antiphagocytic Factors: Only specific examples can be matched accurately; incorrect correlations do not accurately represent their function.
  • Disease Causation Factors: Various factors influence the severity of disease caused by bacteria, such as virulence and host immunity.

Human Defense Mechanisms against Bacteria

  • The body has natural defense mechanisms, but their effectiveness can vary; certain factors do not contribute to defense.

Biofilms and Bacterial Adaptation

  • Bacterial Biofilm Misconceptions: Certain characteristics often attributed to biofilms may not be accurate.
  • Bacteria exhibit adaptive mechanisms to survive, but not all listed options are valid strategies.

Common Misconceptions

  • Understanding which factors mistakenly appear as characteristics of bacteria or biofilms is essential for grasping bacterial pathogenesis and survival mechanisms.

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