Lipid Tests: Solubility, Saponification, and Unsaturation

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10 Questions

What is the principle behind the solubility test?

Solubility of lipids in nonpolar solvents

What is the purpose of using Dichloromethane in the unsaturation test?

To react with double bonds in unsaturated fats

What is the reaction that occurs in the saponification test?

Hydrolysis

What is the purpose of using potassium bisulfite in the acrolein test?

To detect the presence of glycerin

What is the result of a positive test in the grease spot test?

A translucent spot will remain on the filter paper

What is the primary indication of unsaturation in a lipid sample when iodine is added to it?

A color change to colorless or yellow

What is the limitation of the iodine test in determining the characteristics of a lipid sample?

It does not determine physical properties such as melting point and boiling point

What is the result of heating an oil with potassium bisulfate?

Dehydration of the glycerol portion to form Acrolein

What is the characteristic of the smell produced when an oil is heated with potassium bisulfate?

Pungent and resembling burnt oil

What is the primary advantage of the potassium bisulfate test over the iodine test?

It is a more qualitative test that provides a pungent smell

Test your knowledge of various lipid tests, including the Solubility Test, Grease Spot Test, Saponification Test, and Unsaturation Test. Learn about the principles and reagents involved in these tests, and how they are used to detect and analyze lipids.

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