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Lipid Tests: Solubility, Saponification, and Unsaturation

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Lipid Tests: Solubility, Saponification, and Unsaturation

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Questions and Answers

What is the principle behind the solubility test?

Detection of unsaturated fatty acids

Formation of soap or salts of fatty acid

Solubility of lipids in nonpolar solvents (correct)

Reaction with potassium bisulfite

What is the purpose of using Dichloromethane in the unsaturation test?

To detect the presence of glycerin

To form soap or salts of fatty acid

To react with double bonds in unsaturated fats (correct)

To detect the presence of lipids

What is the reaction that occurs in the saponification test?

Polymerization

Oxidation

Esterification

Hydrolysis (correct)

What is the purpose of using potassium bisulfite in the acrolein test?

To detect the presence of glycerin Signup and view all the answers

What is the result of a positive test in the grease spot test?

A translucent spot will remain on the filter paper Signup and view all the answers

What is the primary indication of unsaturation in a lipid sample when iodine is added to it?

A color change to colorless or yellow Signup and view all the answers

What is the limitation of the iodine test in determining the characteristics of a lipid sample?

It does not determine physical properties such as melting point and boiling point Signup and view all the answers

What is the result of heating an oil with potassium bisulfate?

Dehydration of the glycerol portion to form Acrolein Signup and view all the answers

What is the characteristic of the smell produced when an oil is heated with potassium bisulfate?

Pungent and resembling burnt oil Signup and view all the answers

What is the primary advantage of the potassium bisulfate test over the iodine test?

It is a more qualitative test that provides a pungent smell Signup and view all the answers