Lipid Tests: Solubility, Saponification, and Unsaturation
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Questions and Answers

What is the principle behind the solubility test?

  • Detection of unsaturated fatty acids
  • Formation of soap or salts of fatty acid
  • Solubility of lipids in nonpolar solvents (correct)
  • Reaction with potassium bisulfite

What is the purpose of using Dichloromethane in the unsaturation test?

  • To detect the presence of glycerin
  • To form soap or salts of fatty acid
  • To react with double bonds in unsaturated fats (correct)
  • To detect the presence of lipids

What is the reaction that occurs in the saponification test?

  • Polymerization
  • Oxidation
  • Esterification
  • Hydrolysis (correct)

What is the purpose of using potassium bisulfite in the acrolein test?

<p>To detect the presence of glycerin (B)</p> Signup and view all the answers

What is the result of a positive test in the grease spot test?

<p>A translucent spot will remain on the filter paper (D)</p> Signup and view all the answers

What is the primary indication of unsaturation in a lipid sample when iodine is added to it?

<p>A color change to colorless or yellow (A)</p> Signup and view all the answers

What is the limitation of the iodine test in determining the characteristics of a lipid sample?

<p>It does not determine physical properties such as melting point and boiling point (B)</p> Signup and view all the answers

What is the result of heating an oil with potassium bisulfate?

<p>Dehydration of the glycerol portion to form Acrolein (C)</p> Signup and view all the answers

What is the characteristic of the smell produced when an oil is heated with potassium bisulfate?

<p>Pungent and resembling burnt oil (A)</p> Signup and view all the answers

What is the primary advantage of the potassium bisulfate test over the iodine test?

<p>It is a more qualitative test that provides a pungent smell (C)</p> Signup and view all the answers

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