Clinical Microscopy Pre-Boards Exam (Matthew 6:33-34)

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Questions and Answers

It is also known as the FISHBONE diagram; it determines the cause of a problem and identify the different elements that contribute to the problem. It relates the interaction between equipment, methods, and customers.

  • Cause-and-effect diagram (correct)
  • Histogram
  • Pareto chart
  • Flowchart

Based on the principle which states that 80% of the trouble comes from 20% of the problems; this chart is used to mainly identify the problems. The information in this type of graph displays the major contributors to a problem in descending order of importance.

  • Flowchart
  • Histogram
  • Pareto chart (correct)
  • Cause-and-effect diagram

Red diamond or fire hazard, classified according to the Flash Point:

  • A to D
  • 1 to 10
  • 0 to 4 (correct)
  • A to C

Type of extinguisher for flammable organic chemicals:

<p>Class B (B)</p> Signup and view all the answers

Carbon dioxide fire extingushers:

<p>Class B and C fires (B)</p> Signup and view all the answers

Three-dimensional images of high contrast and resolution are obtained, without haloing:

<p>Interference contrast microscopy (B)</p> Signup and view all the answers

Converting a brightfield microscope for polarizing microscopy requires:

<p>Two polarizing filters-one placed below the condenser and one placed between the objective and the eyepiece (A)</p> Signup and view all the answers

Kohler illumination is:

<p>A method to ensure optimal contrast and resolution (A)</p> Signup and view all the answers

How many nephrons are found in the average kidney?

<p>1.3 million (C)</p> Signup and view all the answers

More than 66% of filtered water, sodium, and chloride and 100% of filtered glucose, amino acids, and proteins, are reabsorbed in the:

<p>Proximal tubules (D)</p> Signup and view all the answers

Water reabsorption occurs throughout the nephron except in the:

<p>Ascending limb of the loops of Henle (C)</p> Signup and view all the answers

The final concentration of the urine is determined within the:

<p>Collecting ducts (A)</p> Signup and view all the answers

It corrects renal blood flow in the following ways: causing VASODILATION OF THE AFFERENT ARTERIOLES and CONSTRICTION OF THE EFFERENT ARTERIOLES, stimulating reabsorption of sodium and water in the proximal convoluted tubules, and triggering the release of the sodium-retaining hormone aldosterone by the adrenal cortex and antidiuretic hormone by the hypothalamus:

<p>Angiotensin II (C)</p> Signup and view all the answers

Excellent sediment preservative:

<p>Formalin (D)</p> Signup and view all the answers

For best preservation of urinary sediments, the preservatives of choice are:

<p>Boric acid and thymol (A)</p> Signup and view all the answers

Quantitative urine tests are performed on:

<p>Timed specimens (B)</p> Signup and view all the answers

A negative urine pregnancy test performed on a random specimen may need to be repeated using a:

<p>First morning specimen (C)</p> Signup and view all the answers

The correct method for labeling urine specimen containers is to:

<p>Attach the label to the container (C)</p> Signup and view all the answers

More representative measure of renal concentrating ability can be obtained by measuring:

<p>Osmolarity (D)</p> Signup and view all the answers

The concentration of a normal urine specimen can be estimated by which of the following:

<p>Color (A)</p> Signup and view all the answers

The concentration of a normal urine specimen can be estimated by which of the following?

<p>Glomerular bleeding (C)</p> Signup and view all the answers

The microscopic of a cloudy amber urine is reported as rare WBCs and epithelial cells. What does this suggest?

<p>Possible mix-up of specimen and sediment (D)</p> Signup and view all the answers

Refractive index:

<p>Comparison of the velocity of light in air with the velocity of light in a solution (B)</p> Signup and view all the answers

Refractometer results are valid up to ___; specimen with greater than this SG should be diluted and remeasured.

<p>1.035 (C)</p> Signup and view all the answers

For specific gravity reagent pad: highly buffered alkaline urine may cause low readings, and 0.005 may be added to readings from urines with pH of ___ or greater.

<p>6.5 (C)</p> Signup and view all the answers

High protein diet:

<p>Acid urine (A)</p> Signup and view all the answers

Which of the following tests is affected LEAST by standing or improperly stored urine?

<p>Protein (B)</p> Signup and view all the answers

Leaving a reagent strip in the specimen for too long will:

<p>Cause reagents to leach from the pads (C)</p> Signup and view all the answers

Leaving excess urine on the reagent strip after removing it from the specimen will:

<p>Cause runover between reagent pads (A)</p> Signup and view all the answers

Failure to mix a specimen before inserting the reagent strip will primarily affect the:

<p>Both B and C (D)</p> Signup and view all the answers

Incorporation of the indicator bromthymol blue on the reagent pad MEASURES THE CHANGE IN pH.

<p>Specific gravity (C)</p> Signup and view all the answers

Daily loss of protein in urine normally does not exceed:

<p>150 mg (D)</p> Signup and view all the answers

Glucose oxidase-peroxidase reagent strip and Clinitest result in galactosuria:

<p>Negative reagent strip, positive Clinitest (C)</p> Signup and view all the answers

Ketonuria is associated with increased metabolism of:

<p>Lipids (A)</p> Signup and view all the answers

A speckled pattern on the blood pad of the reagent strip indicates:

<p>Hematuria (A)</p> Signup and view all the answers

Uniform blue color on the blood pad of the reagent strip:

  1. Hematuria.
  2. Hemoglobinuria
  3. Myoglobinuria:

<p>2 and 3 (C)</p> Signup and view all the answers

Medication associated with myoglobinuria:

<p>Statin (C)</p> Signup and view all the answers

Positive result for Ictotest:

<p>Blue to purple mat (A)</p> Signup and view all the answers

Urobilinogen is ___ and labile.

<p>Colorless (A)</p> Signup and view all the answers

One (1) Ehrlich unit is equivalent to:

<p>1 mg/dL (B)</p> Signup and view all the answers

Urine preserved with formalin:

<p>False negative urobilinogen (B)</p> Signup and view all the answers

Hoesch reagent:

<p>Ehrlich reagent dissolved in 6 M HC1 (B)</p> Signup and view all the answers

Positive Greiss reaction:

<p>Escherichia coli (D)</p> Signup and view all the answers

High urine specific gravity:

<p>False negative nitrite (B)</p> Signup and view all the answers

Purple colors are observed in the positive reactions for:

<p>Ketone and leukocytes (B)</p> Signup and view all the answers

Significant Albumin Excretion Rate (AER) :

<p>20 to 200 μg/min (D)</p> Signup and view all the answers

Abnormal result for the ALBUMIN: CREATININE ratio:

<p>30 to 300 mg/g (C)</p> Signup and view all the answers

Differentiation among RBCs, yeast, and oil droplets may be accomplished by all of the following EXCEPT:

<p>Lysis of yeast cells by acetic acid (C)</p> Signup and view all the answers

Glitter cells are neutrophils:

<p>Exposed to urine with low specific gravity (B)</p> Signup and view all the answers

A 22-year-old female clinical laboratory student performs a urinalysis on her own urine as part of a lab class.

  • Color, appearance = yellow, cloudy
  • pH = 7.5
  • Leukocyte esterase = 2+
  • Nitrite = positive Microscopic findings:
  • 25-40 WBC/hpf
  • 0-3 RBC/hpf
  • Moderate bacteria All other chemistries and microscopic results were normal. These findings suggest:

<p>Lower UTI (C)</p> Signup and view all the answers

A 35-year-old patient being treated for a sinus infection with methicillin develops fever, a skin rash, and edema. Urinalysis results are as follows:

  • Color = dark yellow
  • Clarity = cloudy
  • Specific gravity = 1.012
  • pH = 6.0
  • Protein = 3+
  • Glucose = negative
  • Ketones = negative
  • Blood = moderate
  • Bilirubin = negative
  • Urobilinogen = negative
  • Nitrite = negative
  • Leukocyte = 2+ Microscopic:
  • 20 to 30 RBCs/hpf
  • 100 WBCs/hpf

  • 1 to 2 WBC casts/lpf
  • 1 to 2 granular casts/lpf After receiving the urinalysis report, the physician orders eosinophils. The urinary eosinophil result is 10%. What is the probable diagnosis?

<p>Acute interstitial nephritis (C)</p> Signup and view all the answers

Forms of transitional epithelial cells include all of the following except:

<p>Convoluted (C)</p> Signup and view all the answers

The presence of more than ___ RTE cells per high-power field indicates TUBULAR INJURY, and such specimens should be referred for cytologic urine testing.

<blockquote> <p>2 RTE cells/ hpf (B)</p> </blockquote> Signup and view all the answers

Mononuclear leukocytes are sometimes mistaken for:

<p>Renal tubular cells (D)</p> Signup and view all the answers

GRANULAR, DIRTY, BROWN CASTS representing hemoglobin degradation products are associated with:

<p>Acute tubular necrosis (D)</p> Signup and view all the answers

Trichomonas can be seen in a urinary microscopic sample, but cannot be reported unless ___ is observed.

<p>Motility (D)</p> Signup and view all the answers

Fecal contamination of a urine specimen can also result in the presence of ova from intestinal parasites in the urine sediment. The most common contaminant is ova from:

<p>Enterobius vermicularis (B)</p> Signup and view all the answers

These granules are highly refractile spheres, usually with a dimpled center.

<p>Starch granules (B)</p> Signup and view all the answers

Conditions favoring the formation of renal calculi, EXCEPT:

<p>Age (A)</p> Signup and view all the answers

Urinalysis on a patient with severe back pain being evaluated for renal calculi would be most beneficial if it showed:

<p>Microscopic hematuria (D)</p> Signup and view all the answers

Typically, the protocol for the performance of CSF analysis when three tubes are collected is which order for cell counts, chemistries, microbiology?

<p>3, 1 and 2 (C)</p> Signup and view all the answers

Bloody CSF indicative of traumatic tap:

<p>Uneven distribution of blood in 3 collection tubes (C)</p> Signup and view all the answers

Presence of clot in CSF:

  1. Traumatic tap
  2. Froin's syndrome
  3. Tuberculous meningitis
  4. Intracranial hemorrhage

<p>1, 2 and 3 (C)</p> Signup and view all the answers

All of the following proteins are normally present in the CSF except:

<p>Fibrinogen (B)</p> Signup and view all the answers

A decreased CSF total protein concentration may indicate the occurrence of which of the following?

<p>CSF leakage (D)</p> Signup and view all the answers

Eosinophils may be increased in CSF in fungal infections primarily with:

<p>Coccidioides immitis (B)</p> Signup and view all the answers

As little as 0.1 mL of CSF combined with one drop of ___ produces an adequate cell yield when processed with the cytocentrifuge.

<p>30% albumin (D)</p> Signup and view all the answers

For CSF testing, a daily control slide for bacteria should also be prepared using:

<p>0.2 mL saline + 2 drops 30% albumin (D)</p> Signup and view all the answers

CSF cell count should be performed:

<p>Immediately (A)</p> Signup and view all the answers

Compute for the WBC count in patient's CSF. Dilution 1:20, counted in 4 corner squares Given are the number of WBCs in each square: Square 1: 40 Square 2: 43 Square 3: 43 Square 4: 44

<p>8, 500 WBCs/cu.mm. (C)</p> Signup and view all the answers

Arrange the following from the youngest to the most mature stage:

  1. Spermatids
  2. Spermatocytes
  3. Spermatogonia
  4. Spermatozoa

<p>3-2-1-4 (D)</p> Signup and view all the answers

Seminal fluid is collected following a period of sexual abstinence of:

<p>2 to 7 days (C)</p> Signup and view all the answers

A BROWN OR RED HUE IN SEMINAL FLUID may indicate the presence of:

<p>Blood (C)</p> Signup and view all the answers

Compute for the SPERM COUNT:

  • 50 sperms were counted in 5 RBC squares
  • Dilution of 1:10
  • Total volume of seminal fluid is 2 mL

<p>50 M/ejaculate (D)</p> Signup and view all the answers

Measurement of alpha-glucosidase is performed to detect a disorder of the:

<p>Epididymis (B)</p> Signup and view all the answers

Powdered anticoagulants should not be used for synovial fluid because they may produce artifacts that interfere with:

<p>Crystal analysis (B)</p> Signup and view all the answers

Simplest test for synovial fluid viscosity:

<p>String test (D)</p> Signup and view all the answers

Mucin clot test:

<p>Rope's test (D)</p> Signup and view all the answers

Normal synovial fluid glucose:

<p>&lt;10 mg/dL lower than blood glucose (A)</p> Signup and view all the answers

Family history of chromosome abnormalities, such as trisomy 21 (Down syndrome), amniocentesis may be indicated at:

<p>15 to 18 weeks (B)</p> Signup and view all the answers

The amount of amniotic fluid increases in quantity throughout pregnancy, reaching a peak of approximately ___ mL during the third trimester, and then gradually decreases prior to delivery.

<p>800 to 1,200 mL (C)</p> Signup and view all the answers

Which of the following results of a test on the mother would suggest a possible neural tube defect in the fetus?

<p>An α-fetoprotein result of 3.0 MoM (D)</p> Signup and view all the answers

An increased level of AFP in the serum or amniotic fluid of a pregnant woman is seen in:

<p>Open neural tube defects such as spina bifida (C)</p> Signup and view all the answers

Low level of AFP in the serum or amniotic fluid of a pregnant woman is seen in:

<p>Down syndrome (D)</p> Signup and view all the answers

O.D. 650

<p>Lamellar bodies (C)</p> Signup and view all the answers

Pleural fluid triglycerides >110 mg/dL:

<p>Chylous effusion (C)</p> Signup and view all the answers

Lymphocytes in BAL, normally constituting ___ percent of the cell population.

<p>1 to 15% (C)</p> Signup and view all the answers

Induces secretion of hydrochloric acid by parietal cells of the gastric glands:

<p>Gastrin (C)</p> Signup and view all the answers

Failure of the stomach acidity to fall lower than 6.0 in a stimulation test:

<p>Anacidity (D)</p> Signup and view all the answers

Physiologic failure of pH to fall below 3.5 or 1.0 pH unit with gastric stimulation:

<p>Achlorhydria (A)</p> Signup and view all the answers

Physiologic failure of pH to fall below 3.5, although it decreases 1.0 pH unit or more upon gastric stimulation:

<p>Hypochlorhydria (C)</p> Signup and view all the answers

Normal fecal specimen contains:

<p>Water, electrolytes and bacteria (C)</p> Signup and view all the answers

The D-xylose absorption test is used for the differential diagnosis of which two diseases?

<p>Pancreatic insufficiency from malabsorption (A)</p> Signup and view all the answers

Which type of urine sample is needed for a D-xylose absorption test on an adult patient?

<p>5-hour timed urine kept under refrigeration (C)</p> Signup and view all the answers

A patient has been diagnosed with an upper gastrointestinal bleed. Which of the following would be characteristic for this condition?

<p>Stool with a much darker brown/black color (B)</p> Signup and view all the answers

Stool color if patient takes FERROUS SULFATE:

<p>Black (B)</p> Signup and view all the answers

Inability to digest the gelatin in the X-RAY FILM test indicates a deficiency in ___ production.

<p>Trypsin (D)</p> Signup and view all the answers

Which test is MOST SENSITIVE AND SPECIFIC ENZYME in detecting persons with chronic pancreatitis?

<p>Fecal elastase (C)</p> Signup and view all the answers

With the two-slide qualitative fecal fat determination, the first slide produces a normal amount of staining fat present, whereas the second slide, following acid addition and heat, produces an abnormally increased amount of fat. These results indicate:

<p>Malabsorption (A)</p> Signup and view all the answers

Quantitative fecal fat analysis requires a ___ specimen.

<p>72-hour specimen (D)</p> Signup and view all the answers

Flashcards

Cause-and-Effect Diagram

A diagram used to determine the cause of a problem by identifying contributing elements and their interactions.

Pareto Chart

A chart that identifies major contributors to a problem, displaying them in descending order of importance based on the principle that 80% of issues arise from 20% of causes.

Red Diamond Fire Hazard Rating

A hazard level indicator ranging from 0 (minimal hazard) to 4 (severe hazard), based on flash point temperatures.

Class B Extinguisher

A fire extinguisher designed for use on fires involving flammable organic chemicals.

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Carbon Dioxide Extinguishers

Effective for use on Class B (Flammable Liquids) and Class C (Electrical) fires; smothers fire and prevents reignition.

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Interference Contrast Microscopy

Microscopy technique that produces three-dimensional images with high contrast and resolution without haloing.

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Polarizing Microscopy Conversion

To modify brightfield microscopy for polarizing microscopy, you need two polarizing filters: one below the condenser and another between the objective and eyepiece.

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Köhler Illumination

Illumination technique for optimal contrast and resolution in microscopy.

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Nephron Count per Kidney

The average kidney contains approximately 1.3 million of these functional units.

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Proximal Tubule Reabsorption

Most filtered water, sodium, chloride, and all glucose, amino acids, and proteins are reabsorbed here.

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Ascending Limb Impermeability

Water reabsorption occurs throughout the nephron EXCEPT here.

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Collecting Duct Role

The final concentration of urine is determined in these structures.

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Angiotensin II Function

This corrects renal blood flow by vasodilating afferent arterioles, constricting efferent arterioles, stimulating reabsorption of sodium and water, and triggering the release of aldosterone and antidiuretic hormone.

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Formalin

A chemical which helps in preserving urine for microscopic examination.

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Urine Preservatives

Preservatives of choice for preserving urinary sediments.

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Timed Urine Specimens

Urine tests performed over a specific period of time to obtain accurate measurements of substances excreted in urine.

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First Morning Specimen

A negative result from a random urine sample may require repeating the test using this sample type.

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Urine Specimen Labeling

The label should be securely attached to the urine container itself, not the lid.

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Osmolarity Measurement

A more accurate way than specific gravity to assess the kidney's ability to concentrate urine.

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Urine Color as Estimate

A preliminary estimation tool for urine concentration.

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