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Questions and Answers
Describe the structure of chromatin. Include the words histone and nucleosome.
Describe the structure of chromatin. Include the words histone and nucleosome.
Chromatin consists of DNA tightly coiled around proteins called histones. DNA is wrapped around histones to form nucleosomes. The nucleosomes are wrapped up to form chromatin - a thick, ropy fiber.
When must DNA replication occur during the life of a cell? Why?
When must DNA replication occur during the life of a cell? Why?
Before cell division, so that each daughter cell gets a complete copy of the genome.
When do you have actual chromosomes in a cell?
When do you have actual chromosomes in a cell?
Chromosomes condense only for DNA replication; the rest of the time DNA is in chromatin form.
What does the semi-conservative hypothesis of DNA replication mean?
What does the semi-conservative hypothesis of DNA replication mean?
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Describe the DNA replication process in prokaryotes.
Describe the DNA replication process in prokaryotes.
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What are replication forks?
What are replication forks?
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Where does DNA replication begin in eukaryotes? How many of these sites are there?
Where does DNA replication begin in eukaryotes? How many of these sites are there?
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How many bubbles open during DNA replication in prokaryotes and eukaryotes?
How many bubbles open during DNA replication in prokaryotes and eukaryotes?
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What does helicase do?
What does helicase do?
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What are single-stranded binding proteins for?
What are single-stranded binding proteins for?
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What does primase do?
What does primase do?
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What happens to the RNA primer at the end of replication?
What happens to the RNA primer at the end of replication?
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What jobs does DNA replication do?
What jobs does DNA replication do?
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New nucleotides are added to which end of the growing DNA molecule?
New nucleotides are added to which end of the growing DNA molecule?
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New nucleotides added to the 3' end of the growing DNA molecule means the new strand grows in what direction?
New nucleotides added to the 3' end of the growing DNA molecule means the new strand grows in what direction?
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What is the difference between leading and lagging strands? How are they made differently?
What is the difference between leading and lagging strands? How are they made differently?
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What are the lagging strand fragments called?
What are the lagging strand fragments called?
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What must ligase do after primers have been replaced with DNA nucleotides?
What must ligase do after primers have been replaced with DNA nucleotides?
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Which enzyme proofreads DNA for errors?
Which enzyme proofreads DNA for errors?
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Which enzyme cuts out damaged sections of DNA during excision repair?
Which enzyme cuts out damaged sections of DNA during excision repair?
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Do prokaryotes or eukaryotes perform DNA replication more rapidly?
Do prokaryotes or eukaryotes perform DNA replication more rapidly?
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What is the difference between chromatin and chromosomes?
What is the difference between chromatin and chromosomes?
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What are telomeres, where are they located, and what is their purpose?
What are telomeres, where are they located, and what is their purpose?
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How many pairs of chromosomes do humans have? How many total?
How many pairs of chromosomes do humans have? How many total?
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What are homologous chromosomes?
What are homologous chromosomes?
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Explain the difference between autosomes and sex chromosomes.
Explain the difference between autosomes and sex chromosomes.
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Which chromosomes are autosomes? Which are sex chromosomes?
Which chromosomes are autosomes? Which are sex chromosomes?
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XX = ___? XY = ___?
XX = ___? XY = ___?
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Why are new cells made?
Why are new cells made?
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What type of asexual reproduction occurs in prokaryotes? In eukaryotes?
What type of asexual reproduction occurs in prokaryotes? In eukaryotes?
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Mitosis is division of the ___?
Mitosis is division of the ___?
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Give 3 reasons why large cells need to divide. Explain each!
Give 3 reasons why large cells need to divide. Explain each!
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Describe what happens during each of the three parts of interphase.
Describe what happens during each of the three parts of interphase.
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What do centrioles and spindle fibers do?
What do centrioles and spindle fibers do?
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What is the acronym for mitosis only? For the whole cell cycle?
What is the acronym for mitosis only? For the whole cell cycle?
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Do all eukaryotic cells do mitosis?
Do all eukaryotic cells do mitosis?
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Prophase is the longest phase of mitosis. What happens during prophase?
Prophase is the longest phase of mitosis. What happens during prophase?
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What happens during metaphase?
What happens during metaphase?
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What happens during anaphase?
What happens during anaphase?
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What happens during telophase?
What happens during telophase?
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How do plant and animal cells differ in cytokinesis?
How do plant and animal cells differ in cytokinesis?
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How do the daughter cells of mitosis compare to each other and to the parent cell?
How do the daughter cells of mitosis compare to each other and to the parent cell?
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What is density-dependent regulation and how does it work?
What is density-dependent regulation and how does it work?
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What is anchorage dependence?
What is anchorage dependence?
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What are cyclins and what do they do?
What are cyclins and what do they do?
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What are internal regulators?
What are internal regulators?
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What is a checkpoint?
What is a checkpoint?
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What happens at the G1 checkpoint if the cell gets a go-ahead signal? What if it does not?
What happens at the G1 checkpoint if the cell gets a go-ahead signal? What if it does not?
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What is the M checkpoint for?
What is the M checkpoint for?
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What are external regulations?
What are external regulations?
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When are growth factors important?
When are growth factors important?
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What is cancer?
What is cancer?
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Why do cancer cells grow uncontrollably?
Why do cancer cells grow uncontrollably?
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What is a tumor?
What is a tumor?
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Study Notes
Structure of Chromatin
- Chromatin is the combination of DNA and histone proteins.
- DNA is coiled around histones to form nucleosomes, which then aggregate to form chromatin's thick, ropy fibers.
DNA Replication Timing
- Occurs before cell division to ensure each daughter cell receives a complete genome.
Chromosome Formation
- Chromosomes are formed only for DNA replication; otherwise, DNA exists as chromatin.
Semi-Conservative Replication
- Each new DNA strand contains one original strand and one new strand, ensuring fidelity in genetic information.
DNA Replication in Prokaryotes
- Prokaryotic DNA is circular and located in the cytoplasm, replicating from a single point in two directions simultaneously.
- Replication is faster in prokaryotes than in eukaryotes.
Replication Forks
- Origins of replication produce Y-shaped structures called replication forks, where new DNA strands are synthesized.
Eukaryotic DNA Replication
- In eukaryotes, DNA replication starts at hundreds of sites, forming multiple replication bubbles.
Helicase Function
- The helicase enzyme unwinds and separates DNA strands by disrupting hydrogen bonds.
Role of Single-Stranded Binding Proteins
- These proteins maintain the separation and stability of unwound DNA strands.
Primase Enzyme
- Primase synthesizes RNA primers necessary for the initiation of DNA synthesis, approximately 10 nucleotides long.
RNA Primer Replacement
- At the end of replication, RNA primers are removed and replaced with DNA nucleotides by DNA polymerase.
Functions of DNA Replication
- Adds new nucleotides, removes primers, and proofreads DNA for errors.
Direction of Nucleotide Addition
- New nucleotides are added at the 3' end of a growing DNA molecule, meaning synthesis occurs in the 5' to 3' direction.
Leading vs. Lagging Strands
- Leading strands are synthesized continuously towards the replication fork. Lagging strands are synthesized discontinuously, forming Okazaki fragments.
Okazaki Fragments
- Short segments on lagging strands synthesized in a direction opposite to overall replication; require ligase to seal them.
Proofreading Enzyme
- DNA polymerase checks for and corrects errors during DNA replication.
Excision Repair Enzyme
- Nuclease (or exonuclease) removes damaged DNA sections for repair.
Replication Speed
- Prokaryotes replicate DNA more rapidly than eukaryotes.
Chromatin vs. Chromosomes
- Chromatin consists of DNA wound around proteins, whereas chromosomes are tightly coiled chromatin.
Function of Telomeres
- Located at chromosome ends, telomeres prevent gene loss and protect chromosome integrity.
Human Chromosome Count
- Humans have 23 pairs (46 total) of chromosomes.
Homologous Chromosomes
- Pairs of chromosomes (one from each parent) that carry genes for the same traits.
Autosomes vs. Sex Chromosomes
- Autosome pairs (1-22) are identical, while sex chromosomes (23rd pair) differ between genders (XX or XY).
Purpose of New Cell Formation
- New cells are produced for growth, replacement of damaged cells, and reproduction in unicellular organisms.
Asexual Reproduction Types
- Mitosis occurs in eukaryotes, while binary fission occurs in prokaryotes.
Mitosis Definition
- Mitosis is the process of nuclear division.
Reasons for Cell Division
- Large cells experience DNA overload, increased difficulty in material exchange due to surface area-to-volume ratios, and decreased efficiency as they age.
Interphase Stages
- G1 Phase: Growth and synthesis of proteins/organelles.
- S Phase: DNA replication occurs.
- G2 Phase: Further growth and preparation for mitosis.
Centrioles and Spindle Fibers
- Spindle fibers, organized by centrioles, facilitate the separation of chromosomes during cell division.
Mitosis Acronyms
- Mitosis: PMAT
- Entire Cell Cycle: IPMATC
Mitosis in Eukaryotic Cells
- Not all eukaryotic cells undergo mitosis, e.g., nerve and muscle cells.
Prophase Details
- Longest phase of mitosis; chromatin condenses, centrioles align, and the nuclear envelope breaks down.
Metaphase Details
- Chromosomes line up along the cell's equator, connected to spindle fibers.
Anaphase Behavior
- Sister chromatids separate and move towards opposite centrioles.
Telophase Changes
- Chromosomes de-condense, nuclear envelopes re-form, and cytokinesis follows.
Cytokinesis in Different Cells
- Animal cells form cleavage furrow; plant cells form a cell plate for division.
Daughter Cells Post-Mitosis
- Daughter cells are identical to each other and to the parent cell, with matching chromosome counts.
Density-Dependent Regulation
- Cells halt division upon contact with others, which regulates growth and wound healing.
Anchorage Dependence
- Animal cells require attachment to divide, relying on extracellular matrix connections.
Cyclins Function
- Cyclins are proteins that regulate the timing of the cell cycle, present in high amounts during division.
Internal Regulators
- Proteins that ensure certain processes are completed before advancing the cell cycle.
Checkpoints Function
- Critical control points that determine if the cell cycle can proceed based on internal conditions.
G1 Checkpoint Outcomes
- A go-ahead signal leads to cell division; lack of signal results in entry into a non-dividing state (G0).
M Phase Checkpoint Purpose
- Ensures chromosomes are correctly attached to the spindle before anaphase to prevent chromosomal abnormalities.
External Regulators
- Proteins that respond to external cues to control the speed of the cell cycle.
Role of Growth Factors
- External regulators crucial for stimulating growth and division, especially during development and healing.
Cancer Definition
- A disorder characterized by uncontrollable division of certain cells.
Cancer Cell Behavior
- Cancer cells ignore growth regulation signals, leading to uncontrolled proliferation.
Tumors
- Masses formed by the uncontrolled growth of cancer cells.
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