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Untitled Quiz

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Questions and Answers

Describe the structure of chromatin. Include the words histone and nucleosome.

Chromatin consists of DNA tightly coiled around proteins called histones. DNA is wrapped around histones to form nucleosomes. The nucleosomes are wrapped up to form chromatin - a thick, ropy fiber.

When must DNA replication occur during the life of a cell? Why?

Before cell division, so that each daughter cell gets a complete copy of the genome.

When do you have actual chromosomes in a cell?

Chromosomes condense only for DNA replication; the rest of the time DNA is in chromatin form.

What does the semi-conservative hypothesis of DNA replication mean?

<p>A parent strand is used as a model while new nucleotides attach. This means that each new piece of DNA has one original strand and one new strand.</p> Signup and view all the answers

Describe the DNA replication process in prokaryotes.

<p>In prokaryotic cells, DNA is located in the cytoplasm. Most prokaryotes have a single, circular DNA molecule containing nearly all of the cell's genetic information. Replication begins at a single point and proceeds in two directions simultaneously until the DNA is copied. It is faster than DNA replication in eukaryotes.</p> Signup and view all the answers

What are replication forks?

<p>DNA replication begins at origins of replication. Two strands open forming replication forks (Y-shaped region). New strands grow at the forks.</p> Signup and view all the answers

Where does DNA replication begin in eukaryotes? How many of these sites are there?

<p>In eukaryotic chromosomes, DNA replication occurs at hundreds of places.</p> Signup and view all the answers

How many bubbles open during DNA replication in prokaryotes and eukaryotes?

<p>Many in eukaryotes</p> Signup and view all the answers

What does helicase do?

<p>Enzyme helicase unwinds and separates the 2 DNA strands by breaking the weak hydrogen bonds.</p> Signup and view all the answers

What are single-stranded binding proteins for?

<p>Single-Stranded Binding Proteins attach and keep the 2 DNA strands separated and untwisted (like thumb tacks).</p> Signup and view all the answers

What does primase do?

<p>Primase is the enzyme that synthesizes the RNA primer needed to start the addition of new nucleotides.</p> Signup and view all the answers

What happens to the RNA primer at the end of replication?

<p>The primer will be removed by DNA polymerase and replaced with DNA nucleotides.</p> Signup and view all the answers

What jobs does DNA replication do?

<p>It adds new DNA nucleotides, removes primers, and proofreads the DNA for errors.</p> Signup and view all the answers

New nucleotides are added to which end of the growing DNA molecule?

<p>3' end.</p> Signup and view all the answers

New nucleotides added to the 3' end of the growing DNA molecule means the new strand grows in what direction?

<p>5' —&gt; 3'.</p> Signup and view all the answers

What is the difference between leading and lagging strands? How are they made differently?

<p>Leading strands are synthesized continuously toward the opening replication fork, while lagging strands are synthesized discontinuously in fragments called Okazaki fragments.</p> Signup and view all the answers

What are the lagging strand fragments called?

<p>Okazaki fragments.</p> Signup and view all the answers

What must ligase do after primers have been replaced with DNA nucleotides?

<p>All segments of the sugar-phosphate backbone are sealed by ligase.</p> Signup and view all the answers

Which enzyme proofreads DNA for errors?

<p>DNA polymerase.</p> Signup and view all the answers

Which enzyme cuts out damaged sections of DNA during excision repair?

<p>Nuclease (exonuclease).</p> Signup and view all the answers

Do prokaryotes or eukaryotes perform DNA replication more rapidly?

<p>Prokaryotes.</p> Signup and view all the answers

What is the difference between chromatin and chromosomes?

<p>Chromosomes are supercoiled chromatin; chromatin is DNA wound around proteins.</p> Signup and view all the answers

What are telomeres, where are they located, and what is their purpose?

<p>Telomeres are repeating segments of DNA located at both ends of a chromosome. They ensure that all genes are replicated and not lost, preventing the ends of chromosomes from fraying or sticking together.</p> Signup and view all the answers

How many pairs of chromosomes do humans have? How many total?

<p>23 pairs; 46 total.</p> Signup and view all the answers

What are homologous chromosomes?

<p>Pairs of chromosomes (maternal and paternal) that are similar in shape and size.</p> Signup and view all the answers

Explain the difference between autosomes and sex chromosomes.

<p>22 of the homologous pairs match more or less exactly (the autosomes); the 23rd pair (sex chromosomes) will match if it's XX (female) but be different if it's XY (male).</p> Signup and view all the answers

Which chromosomes are autosomes? Which are sex chromosomes?

<p>In humans, the autosomes are sets of 1-22, and the 23rd set of chromosomes are sex chromosomes.</p> Signup and view all the answers

XX = ___? XY = ___?

<p>XX = female; XY = male.</p> Signup and view all the answers

Why are new cells made?

<p>New cells are produced for growth and to replace damaged or old cells (or in unicellular organisms, for reproduction).</p> Signup and view all the answers

What type of asexual reproduction occurs in prokaryotes? In eukaryotes?

<p>Eukaryotes: Mitosis. Prokaryotes: Binary fission.</p> Signup and view all the answers

Mitosis is division of the ___?

<p>Nucleus/genetic material.</p> Signup and view all the answers

Give 3 reasons why large cells need to divide. Explain each!

<ol> <li>Large cells experience DNA overload; as a cell grows, it has the same amount of DNA for increased responsibilities. 2. They can't efficiently exchange materials; the surface area to volume ratio decreases as the cell enlarges. 3. Aging cells are less efficient.</li> </ol> Signup and view all the answers

Describe what happens during each of the three parts of interphase.

<p>G1 Phase = Primary Growth: cells grow and synthesize proteins and organelles. S Phase = Synthesis: cells replicate DNA. G2 Phase = Secondary Growth: the cell grows slightly more and prepares for mitosis.</p> Signup and view all the answers

What do centrioles and spindle fibers do?

<p>Centrioles and spindle fibers are part of the cytoskeleton. Spindle fibers connect to chromosomes to separate them during cell division.</p> Signup and view all the answers

What is the acronym for mitosis only? For the whole cell cycle?

<p>Mitosis: PMAT; Cell Cycle: IPMATC.</p> Signup and view all the answers

Do all eukaryotic cells do mitosis?

<p>False</p> Signup and view all the answers

Prophase is the longest phase of mitosis. What happens during prophase?

<p>During prophase, the centrioles lineup on opposite sides of the nucleus, spindle fibers form, chromatin condenses into visible chromosomes, and the nucleolus disappears.</p> Signup and view all the answers

What happens during metaphase?

<p>During metaphase, chromosomes line up across the center of the cell and microtubules connect the centromere of each chromosome to the spindle fibers.</p> Signup and view all the answers

What happens during anaphase?

<p>During anaphase, centromeres separate and chromatids become individual chromosomes that move apart until they reach the centrioles.</p> Signup and view all the answers

What happens during telophase?

<p>During telophase, chromosomes tangle back into chromatin, a nuclear envelope re-forms around each chromosome set, spindle breaks apart, and nucleoli become visible.</p> Signup and view all the answers

How do plant and animal cells differ in cytokinesis?

<p>In animals, cytokinesis occurs by a cleavage furrow where the cell membrane is drawn in. In plants, a cell plate forms between the new nuclei.</p> Signup and view all the answers

How do the daughter cells of mitosis compare to each other and to the parent cell?

<p>Daughter cells have the same number of chromosomes and genes as the parent cell, identical to each other but smaller than the parent cell.</p> Signup and view all the answers

What is density-dependent regulation and how does it work?

<p>Density-dependent regulation occurs when cells stop growing upon contact with other cells; they will divide to fill gaps and stop when touching neighbor cells.</p> Signup and view all the answers

What is anchorage dependence?

<p>Anchorage dependence is when most animal cells require attachment to a substratum to divide.</p> Signup and view all the answers

What are cyclins and what do they do?

<p>Cyclins are proteins that regulate the timing of the cell cycle in eukaryotic cells, present in high amounts during division.</p> Signup and view all the answers

What are internal regulators?

<p>Internal regulators are proteins that respond to internal cell events and stop the cycle at checkpoints.</p> Signup and view all the answers

What is a checkpoint?

<p>A checkpoint is a critical control point in the cell cycle where stop and go signals regulate progression.</p> Signup and view all the answers

What happens at the G1 checkpoint if the cell gets a go-ahead signal? What if it does not?

<p>If the cell receives a go-ahead signal, it continues to divide; if not, it exits to a non-dividing state (G0 phase).</p> Signup and view all the answers

What is the M checkpoint for?

<p>The M phase checkpoint ensures all chromosomes are attached to the spindle before moving to anaphase.</p> Signup and view all the answers

What are external regulations?

<p>External regulators are proteins that respond to external events and direct the cell cycle to speed up or slow down.</p> Signup and view all the answers

When are growth factors important?

<p>Growth factors are important external regulators that stimulate growth and division, particularly during development and healing.</p> Signup and view all the answers

What is cancer?

<p>Cancer is a disorder in which some of the body's cells lose the ability to control growth.</p> Signup and view all the answers

Why do cancer cells grow uncontrollably?

<p>Cancer cells do not respond to the signals that regulate growth in normal cells.</p> Signup and view all the answers

What is a tumor?

<p>A tumor is a mass of cancer cells.</p> Signup and view all the answers

Study Notes

Structure of Chromatin

  • Chromatin is the combination of DNA and histone proteins.
  • DNA is coiled around histones to form nucleosomes, which then aggregate to form chromatin's thick, ropy fibers.

DNA Replication Timing

  • Occurs before cell division to ensure each daughter cell receives a complete genome.

Chromosome Formation

  • Chromosomes are formed only for DNA replication; otherwise, DNA exists as chromatin.

Semi-Conservative Replication

  • Each new DNA strand contains one original strand and one new strand, ensuring fidelity in genetic information.

DNA Replication in Prokaryotes

  • Prokaryotic DNA is circular and located in the cytoplasm, replicating from a single point in two directions simultaneously.
  • Replication is faster in prokaryotes than in eukaryotes.

Replication Forks

  • Origins of replication produce Y-shaped structures called replication forks, where new DNA strands are synthesized.

Eukaryotic DNA Replication

  • In eukaryotes, DNA replication starts at hundreds of sites, forming multiple replication bubbles.

Helicase Function

  • The helicase enzyme unwinds and separates DNA strands by disrupting hydrogen bonds.

Role of Single-Stranded Binding Proteins

  • These proteins maintain the separation and stability of unwound DNA strands.

Primase Enzyme

  • Primase synthesizes RNA primers necessary for the initiation of DNA synthesis, approximately 10 nucleotides long.

RNA Primer Replacement

  • At the end of replication, RNA primers are removed and replaced with DNA nucleotides by DNA polymerase.

Functions of DNA Replication

  • Adds new nucleotides, removes primers, and proofreads DNA for errors.

Direction of Nucleotide Addition

  • New nucleotides are added at the 3' end of a growing DNA molecule, meaning synthesis occurs in the 5' to 3' direction.

Leading vs. Lagging Strands

  • Leading strands are synthesized continuously towards the replication fork. Lagging strands are synthesized discontinuously, forming Okazaki fragments.

Okazaki Fragments

  • Short segments on lagging strands synthesized in a direction opposite to overall replication; require ligase to seal them.

Proofreading Enzyme

  • DNA polymerase checks for and corrects errors during DNA replication.

Excision Repair Enzyme

  • Nuclease (or exonuclease) removes damaged DNA sections for repair.

Replication Speed

  • Prokaryotes replicate DNA more rapidly than eukaryotes.

Chromatin vs. Chromosomes

  • Chromatin consists of DNA wound around proteins, whereas chromosomes are tightly coiled chromatin.

Function of Telomeres

  • Located at chromosome ends, telomeres prevent gene loss and protect chromosome integrity.

Human Chromosome Count

  • Humans have 23 pairs (46 total) of chromosomes.

Homologous Chromosomes

  • Pairs of chromosomes (one from each parent) that carry genes for the same traits.

Autosomes vs. Sex Chromosomes

  • Autosome pairs (1-22) are identical, while sex chromosomes (23rd pair) differ between genders (XX or XY).

Purpose of New Cell Formation

  • New cells are produced for growth, replacement of damaged cells, and reproduction in unicellular organisms.

Asexual Reproduction Types

  • Mitosis occurs in eukaryotes, while binary fission occurs in prokaryotes.

Mitosis Definition

  • Mitosis is the process of nuclear division.

Reasons for Cell Division

  • Large cells experience DNA overload, increased difficulty in material exchange due to surface area-to-volume ratios, and decreased efficiency as they age.

Interphase Stages

  • G1 Phase: Growth and synthesis of proteins/organelles.
  • S Phase: DNA replication occurs.
  • G2 Phase: Further growth and preparation for mitosis.

Centrioles and Spindle Fibers

  • Spindle fibers, organized by centrioles, facilitate the separation of chromosomes during cell division.

Mitosis Acronyms

  • Mitosis: PMAT
  • Entire Cell Cycle: IPMATC

Mitosis in Eukaryotic Cells

  • Not all eukaryotic cells undergo mitosis, e.g., nerve and muscle cells.

Prophase Details

  • Longest phase of mitosis; chromatin condenses, centrioles align, and the nuclear envelope breaks down.

Metaphase Details

  • Chromosomes line up along the cell's equator, connected to spindle fibers.

Anaphase Behavior

  • Sister chromatids separate and move towards opposite centrioles.

Telophase Changes

  • Chromosomes de-condense, nuclear envelopes re-form, and cytokinesis follows.

Cytokinesis in Different Cells

  • Animal cells form cleavage furrow; plant cells form a cell plate for division.

Daughter Cells Post-Mitosis

  • Daughter cells are identical to each other and to the parent cell, with matching chromosome counts.

Density-Dependent Regulation

  • Cells halt division upon contact with others, which regulates growth and wound healing.

Anchorage Dependence

  • Animal cells require attachment to divide, relying on extracellular matrix connections.

Cyclins Function

  • Cyclins are proteins that regulate the timing of the cell cycle, present in high amounts during division.

Internal Regulators

  • Proteins that ensure certain processes are completed before advancing the cell cycle.

Checkpoints Function

  • Critical control points that determine if the cell cycle can proceed based on internal conditions.

G1 Checkpoint Outcomes

  • A go-ahead signal leads to cell division; lack of signal results in entry into a non-dividing state (G0).

M Phase Checkpoint Purpose

  • Ensures chromosomes are correctly attached to the spindle before anaphase to prevent chromosomal abnormalities.

External Regulators

  • Proteins that respond to external cues to control the speed of the cell cycle.

Role of Growth Factors

  • External regulators crucial for stimulating growth and division, especially during development and healing.

Cancer Definition

  • A disorder characterized by uncontrollable division of certain cells.

Cancer Cell Behavior

  • Cancer cells ignore growth regulation signals, leading to uncontrolled proliferation.

Tumors

  • Masses formed by the uncontrolled growth of cancer cells.

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