Lac Operon and Pseudo-Operators
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Questions and Answers

What is the role of the 'pseudo-operator' sequences within the lac operon sequence?

  • Contribute to repression by binding LacI more weakly (correct)
  • Prevent the binding of LacI to LacO
  • Act as an alternative operator for LacI binding
  • Enhance gene expression by binding LacI more strongly

    • How does the presence of two DNA-binding sites in LacI protein tetramer enhance repression?

  • One LacI tetramer could bind two separate operators and generate a looped DNA structure (correct)
  • It promotes the transcription of the lac operon
  • It prevents the binding of LacI to LacO
  • It allows for the simultaneous binding of multiple repressor proteins

    • What experimental evidence supports the concept of DNA looping in the lac operon?

  • Evidence obtained from a variety of laboratories (correct)
  • Observation of enhanced gene expression in lac operon mutants
  • The presence of additional regulatory elements in the lac operon
  • The absence of pseudo-operators in mutant lac operons

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