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Questions and Answers
What is the role of the 'pseudo-operator' sequences within the lac operon sequence?
What is the role of the 'pseudo-operator' sequences within the lac operon sequence?
- Contribute to repression by binding LacI more weakly (correct)
- Prevent the binding of LacI to LacO
- Act as an alternative operator for LacI binding
- Enhance gene expression by binding LacI more strongly
How does the presence of two DNA-binding sites in LacI protein tetramer enhance repression?
How does the presence of two DNA-binding sites in LacI protein tetramer enhance repression?
- One LacI tetramer could bind two separate operators and generate a looped DNA structure (correct)
- It promotes the transcription of the lac operon
- It prevents the binding of LacI to LacO
- It allows for the simultaneous binding of multiple repressor proteins
What experimental evidence supports the concept of DNA looping in the lac operon?
What experimental evidence supports the concept of DNA looping in the lac operon?
- Evidence obtained from a variety of laboratories (correct)
- Observation of enhanced gene expression in lac operon mutants
- The presence of additional regulatory elements in the lac operon
- The absence of pseudo-operators in mutant lac operons
