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Questions and Answers
The blood sample used in the procedure is 5 ml
False
The 0.3N BaOH solution is prepared by dissolving 9.46 g of BaOH in 50 ml of dH2O
False
The standard glucose solution has a concentration of 100 mg/dL
False
The supernatant is collected after centrifugation at 2000g for 10 minutes
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The 5% ZnSO4 solution is prepared by dissolving 10 g of ZnSO4 in 100 ml of dH2O
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The plasma is separated from the blood sample by centrifugation at 2000g for 10 minutes
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The alkaline copper tartrate solution is prepared by mixing 96 mL of solution A and 4 mL of solution B.
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The arsenomolybdate reagent is prepared by dissolving 5g of ammonium molybdate in 45mL water.
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The session for determining reducing sugars will be conducted in 3 separate sessions with 5 groups per session.
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The reducing sugar determination method uses a visible light spectrophotometer with maximum transmission in the region of 520 nm.
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The quartz, glass or polystyrene cuvettes are used for storing the blood samples.
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The lancet is used to collect blood samples from the volunteers.
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The working glucose standard solution is prepared by making a 1000x dilution of the glucose standard solution provided.
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Alkaline copper tartrate reagent is added to the tubes after centrifugation.
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The absorbance is read using a spectrophotometer at a wavelength of 540 nm.
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The tubes are placed in an ice bath for 10 minutes.
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Arsenomolybdate reagent is added to the tubes before adding alkaline copper tartrate reagent.
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The capillary blood glucose level is measured using a spectrophotometer.
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