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Laboratory Sampling Techniques

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18 Questions

What is a characteristic of Arterial Puncture?

Higher risk

What is an advantage of POCT?

Is safer

What type of needle is used in the Vacutainer Method?

Double-sided needle

What is an advantage of the Syringe Method?

Requires a smaller amount of blood

What type of sample is typically analyzed using Urinalysis?

Urine

What is a type of sample that may be analyzed using Cytology?

All of the above

What is the purpose of using 10% Formalin in Histology?

To preserve the sample

What is a consideration when selecting a needle length for a Syringe Method?

Site of puncture

What is the color of the blood tube used for collecting blood samples for glucose tests?

Gray

Which of the following tests is not performed on a blue top blood tube?

CBC

What is the purpose of the anticoagulant in a purple top blood tube?

To separate plasma from blood cells

Which department is responsible for performing lipid profile tests?

Clinical Chemistry

What is the purpose of the green top blood tube?

To collect blood samples for chemistry tests

Which of the following tests is not performed on a red or yellow top blood tube?

PT test

What is the purpose of sodium citrate in a blue top blood tube?

To prevent blood clotting

Which department is responsible for performing HIV tests?

Immunology

What is the purpose of EDTA in a purple top blood tube?

To prevent blood clotting

Which of the following tests is performed on a green top blood tube?

All chemistries

Study Notes

Laboratory Samples

  • Laboratory samples can be classified into two categories: blood and non-blood samples.
  • Blood samples can be further divided into arterial, venipuncture, and capillary samples.

Blood Collection Methods

  • Syringe method: uses a slip tip or luer lock syringe, available in various capacities (3, 5, 10, 20, 60 cc), and needles with different gauges and lengths.
  • Vacutainer method: uses a double-sided needle and a vacuum-dependent system.

Blood Tubes

  • Anticoagulated blood tubes: used for plasma samples, available in different colors, each with specific anticoagulants and additives.
    • Gray top: contains sodium fluoride and potassium oxalate, used for glucose tests.
    • Purple top: contains EDTA, used for CBC, ESR, and sickle cell tests.
    • Blue top: contains sodium citrate, used for PT, PTT, and INR tests.
    • Green top: contains lithium heparin, used for chemistry tests.
  • Non-anticoagulated blood tubes: used for serum samples, available in red or yellow (SST) tubes.
    • Red or yellow top: used for lipid profile, renal function, liver function, and other tests.

Common Tests and Departments

  • Clinical Chemistry: glucose, lipid profile, renal function, liver function, and other tests.
  • Hematology: CBC, ESR, sickle cell test, and blood type.
  • Coagulation/Hematology/Clinical Chemistry: PT, PTT, INR, D-DIMER tests.
  • Immunology: ELISA, CLIA, microbead, and IF tests for various analytes.

Non-Blood Samples

  • Urine: used for urinalysis, 24-hour urine analysis, and microscopy.
  • Stool: used for microscopy, analysis, and count.
  • Sputum: used for analysis and count.
  • Semen: used for analysis and count.
  • Swabs: used for throat, wound, and rectal swab analysis.
  • Fluids: used for synovial, pleural, CSF, and other fluid analysis.
  • Cytology: used for histology and cytology tests.
  • Histology: used for tissue analysis, typically with 10% formalin.

This quiz covers the different methods of collecting laboratory samples, including blood, non-blood, and specialized samples. It explores the various techniques and risks associated with each method.

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