Laboratory Sampling Techniques

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Questions and Answers

What is a characteristic of Arterial Puncture?

Safer

Larger amount of blood

Higher risk (correct)

Less invasive

What is an advantage of POCT?

Requires specialized training

Is safer (correct)

Requires a larger amount of blood

Is more invasive

What type of needle is used in the Vacutainer Method?

Double-sided needle (correct)

Slip Tip

Luer Lock

Single-sided needle

What is an advantage of the Syringe Method?

Requires a smaller amount of blood Signup and view all the answers

What type of sample is typically analyzed using Urinalysis?

Urine Signup and view all the answers

What is a type of sample that may be analyzed using Cytology?

All of the above Signup and view all the answers

What is the purpose of using 10% Formalin in Histology?

To preserve the sample Signup and view all the answers

What is a consideration when selecting a needle length for a Syringe Method?

Site of puncture Signup and view all the answers

What is the color of the blood tube used for collecting blood samples for glucose tests?

Gray Signup and view all the answers

Which of the following tests is not performed on a blue top blood tube?

CBC Signup and view all the answers

What is the purpose of the anticoagulant in a purple top blood tube?

To separate plasma from blood cells Signup and view all the answers

Which department is responsible for performing lipid profile tests?

Clinical Chemistry Signup and view all the answers

What is the purpose of the green top blood tube?

To collect blood samples for chemistry tests Signup and view all the answers

Which of the following tests is not performed on a red or yellow top blood tube?

PT test Signup and view all the answers

What is the purpose of sodium citrate in a blue top blood tube?

To prevent blood clotting Signup and view all the answers

Which department is responsible for performing HIV tests?

Immunology Signup and view all the answers

What is the purpose of EDTA in a purple top blood tube?

To prevent blood clotting Signup and view all the answers

Which of the following tests is performed on a green top blood tube?

All chemistries Signup and view all the answers

Study Notes

Laboratory Samples

Laboratory samples can be classified into two categories: blood and non-blood samples.
Blood samples can be further divided into arterial, venipuncture, and capillary samples.

Blood Collection Methods

Syringe method: uses a slip tip or luer lock syringe, available in various capacities (3, 5, 10, 20, 60 cc), and needles with different gauges and lengths.
Vacutainer method: uses a double-sided needle and a vacuum-dependent system.

Blood Tubes

Anticoagulated blood tubes: used for plasma samples, available in different colors, each with specific anticoagulants and additives.
- Gray top: contains sodium fluoride and potassium oxalate, used for glucose tests.
- Purple top: contains EDTA, used for CBC, ESR, and sickle cell tests.
- Blue top: contains sodium citrate, used for PT, PTT, and INR tests.
- Green top: contains lithium heparin, used for chemistry tests.
Non-anticoagulated blood tubes: used for serum samples, available in red or yellow (SST) tubes.
- Red or yellow top: used for lipid profile, renal function, liver function, and other tests.

Common Tests and Departments

Clinical Chemistry: glucose, lipid profile, renal function, liver function, and other tests.
Hematology: CBC, ESR, sickle cell test, and blood type.
Coagulation/Hematology/Clinical Chemistry: PT, PTT, INR, D-DIMER tests.
Immunology: ELISA, CLIA, microbead, and IF tests for various analytes.

Non-Blood Samples

Urine: used for urinalysis, 24-hour urine analysis, and microscopy.
Stool: used for microscopy, analysis, and count.
Sputum: used for analysis and count.
Semen: used for analysis and count.
Swabs: used for throat, wound, and rectal swab analysis.
Fluids: used for synovial, pleural, CSF, and other fluid analysis.
Cytology: used for histology and cytology tests.
Histology: used for tissue analysis, typically with 10% formalin.

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Description

This quiz covers the different methods of collecting laboratory samples, including blood, non-blood, and specialized samples. It explores the various techniques and risks associated with each method.