Laboratory Sampling Techniques

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Questions and Answers

What is a characteristic of Arterial Puncture?

  • Safer
  • Larger amount of blood
  • Higher risk (correct)
  • Less invasive

What is an advantage of POCT?

  • Requires specialized training
  • Is safer (correct)
  • Requires a larger amount of blood
  • Is more invasive

What type of needle is used in the Vacutainer Method?

  • Double-sided needle (correct)
  • Slip Tip
  • Luer Lock
  • Single-sided needle

What is an advantage of the Syringe Method?

<p>Requires a smaller amount of blood (B)</p> Signup and view all the answers

What type of sample is typically analyzed using Urinalysis?

<p>Urine (C)</p> Signup and view all the answers

What is a type of sample that may be analyzed using Cytology?

<p>All of the above (D)</p> Signup and view all the answers

What is the purpose of using 10% Formalin in Histology?

<p>To preserve the sample (A)</p> Signup and view all the answers

What is a consideration when selecting a needle length for a Syringe Method?

<p>Site of puncture (B)</p> Signup and view all the answers

What is the color of the blood tube used for collecting blood samples for glucose tests?

<p>Gray (B)</p> Signup and view all the answers

Which of the following tests is not performed on a blue top blood tube?

<p>CBC (C)</p> Signup and view all the answers

What is the purpose of the anticoagulant in a purple top blood tube?

<p>To separate plasma from blood cells (D)</p> Signup and view all the answers

Which department is responsible for performing lipid profile tests?

<p>Clinical Chemistry (A)</p> Signup and view all the answers

What is the purpose of the green top blood tube?

<p>To collect blood samples for chemistry tests (A)</p> Signup and view all the answers

Which of the following tests is not performed on a red or yellow top blood tube?

<p>PT test (B)</p> Signup and view all the answers

What is the purpose of sodium citrate in a blue top blood tube?

<p>To prevent blood clotting (A)</p> Signup and view all the answers

Which department is responsible for performing HIV tests?

<p>Immunology (D)</p> Signup and view all the answers

What is the purpose of EDTA in a purple top blood tube?

<p>To prevent blood clotting (B)</p> Signup and view all the answers

Which of the following tests is performed on a green top blood tube?

<p>All chemistries (C)</p> Signup and view all the answers

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Study Notes

Laboratory Samples

  • Laboratory samples can be classified into two categories: blood and non-blood samples.
  • Blood samples can be further divided into arterial, venipuncture, and capillary samples.

Blood Collection Methods

  • Syringe method: uses a slip tip or luer lock syringe, available in various capacities (3, 5, 10, 20, 60 cc), and needles with different gauges and lengths.
  • Vacutainer method: uses a double-sided needle and a vacuum-dependent system.

Blood Tubes

  • Anticoagulated blood tubes: used for plasma samples, available in different colors, each with specific anticoagulants and additives.
    • Gray top: contains sodium fluoride and potassium oxalate, used for glucose tests.
    • Purple top: contains EDTA, used for CBC, ESR, and sickle cell tests.
    • Blue top: contains sodium citrate, used for PT, PTT, and INR tests.
    • Green top: contains lithium heparin, used for chemistry tests.
  • Non-anticoagulated blood tubes: used for serum samples, available in red or yellow (SST) tubes.
    • Red or yellow top: used for lipid profile, renal function, liver function, and other tests.

Common Tests and Departments

  • Clinical Chemistry: glucose, lipid profile, renal function, liver function, and other tests.
  • Hematology: CBC, ESR, sickle cell test, and blood type.
  • Coagulation/Hematology/Clinical Chemistry: PT, PTT, INR, D-DIMER tests.
  • Immunology: ELISA, CLIA, microbead, and IF tests for various analytes.

Non-Blood Samples

  • Urine: used for urinalysis, 24-hour urine analysis, and microscopy.
  • Stool: used for microscopy, analysis, and count.
  • Sputum: used for analysis and count.
  • Semen: used for analysis and count.
  • Swabs: used for throat, wound, and rectal swab analysis.
  • Fluids: used for synovial, pleural, CSF, and other fluid analysis.
  • Cytology: used for histology and cytology tests.
  • Histology: used for tissue analysis, typically with 10% formalin.

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