Questions and Answers
What is a key characteristic of flammable liquids?
Which type of chemical must be stored separately from mineral acids?
What immediate action should be taken if a chemical spill occurs in the lab?
Which color-coded waste container is used for infectious and pathological waste?
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What distinguishes a combustible liquid from a flammable liquid?
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Which type of substance is classified as carcinogenic?
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Which chemical class can react violently with water?
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Which of the following statements regarding lab safety procedures is false?
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What is the preferred method for handling specimens during centrifugation to minimize the risk of aerosol exposure?
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Which personal protective equipment (PPE) is essential when cleaning up a biological spill?
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What is the first step to take when a spill occurs in the laboratory?
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How should you dispose of materials used in a biological spill cleanup?
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Which of the following methods is recommended for cleaning a spill site after using an absorbent material?
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What is the CDC requirement related to airborne pathogens in laboratories?
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What must an Exposure Control Plan include for employees working with bloodborne pathogens?
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Which action is NOT recommended when managing a laboratory spill?
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What is the maximum operating sash height that should be established for a chemical fume hood?
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Which type of personal protective equipment is NOT recommended when handling chemical reagents?
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Which of the following is a requirement for a safety shower in a laboratory?
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What factor determines the level of protection offered by a Biosafety Cabinet (BSC)?
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How often should face velocity in a fume hood be evaluated?
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What is the recommended travel distance to an eyewash station in areas with chemical exposure hazards?
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What is NOT a characteristic of chemical fume hoods?
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Which of the following should you do first when donning PPE?
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What should be done during the doffing process after removing gloves?
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What is the primary purpose of spill kits in the laboratory?
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Study Notes
Chemical Storage Requirements
- Chemicals must be organized by class in designated areas separate from routine workspaces.
- Flammable liquids and solids, mineral and organic acids, caustics, oxidizers, perchloric acid, water-reactive, air-reactive, heat-reactive, and unstable substances should all be stored separately.
Chemical Safety
- Flammable Chemicals: Classified by flash point; flammable (<37.8°C) vs. combustible (≥37.8°C).
- Corrosive Chemicals: Can cause severe burns upon contact with skin or eyes; require immediate flushing with water.
- Reactive Chemicals: May spontaneously ignite or explode under certain conditions.
- Carcinogenic Chemicals: Identified as cancer-causing agents.
Waste Disposal Protocols
- Black Containers: For non-infectious dry waste.
- Green Containers: For non-infectious wet waste.
- Yellow Containers: For infectious and pathological materials.
- Yellow with Black Band: For chemical waste or heavy metals.
- Orange Containers: For radioactive waste.
- Red Containers: For sharps disposal.
Spill Response Procedures
- Alert personnel in the vicinity of a spill and wear appropriate protective gear.
- Use mechanical devices for sharp objects and absorb spills with absorbent materials.
- Clean spills with detergent and disinfectant, rinse with water, and dispose of materials in biohazard containers.
Biologic Safety Protocols
- Exposure Control Plans are essential to minimize risks associated with bloodborne pathogens; must be accessible to all employees.
- TB exposure control programs are required in laboratories involved in diagnosing or treating TB.
Safety Equipment Regulations
- Employers must provide safety equipment such as showers, eyewash stations, and fire extinguishers, with inspections conducted regularly.
- Safety showers should deliver 30-50 gallons/minute at specified pressure; eyewash stations must be within 100 feet of hazards.
Biosafety Cabinets
- Designed to protect laboratory workers from harmful particles, with varying protection levels based on biosafety needs.
- The CDC outlines four biosafety levels corresponding to different laboratory risks.
Chemical Fume Hoods
- Essential for containing hazardous chemicals; should never be operated with the sash fully open.
- Regular evaluation of hood ventilation is necessary to maintain a face velocity of 100-120 feet per minute.
Personal Protective Equipment (PPE)
- Required PPE includes safety glasses, goggles, laboratory coats, gloves, and respirators.
- Proper donning sequence: gown, mask, goggles, gloves.
- Doffing sequence to prevent contamination: gloves first, followed by goggles, mask, gown, and handwashing.
General Biologic Safety Practices
- Follow standard precautions for handling blood samples and body fluids, ensuring safe transport and processing.
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Description
Test your knowledge on laboratory safety protocols and chemical storage requirements. This quiz covers essential practices for isolating hazardous substances and ensuring a safe work environment. Ideal for students and professionals in laboratory settings.