Laboratory Safety Guidelines Quiz
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Questions and Answers

What should you do if a chemical is spilled on you?

  • Report it after class
  • Leave it to dry
  • Wash it off immediately with water (correct)
  • Ignore it

It is permissible to eat or drink in the laboratory.

False (B)

What type of clothing is required for working in the laboratory?

Protective clothing, such as a lab coat

The formula weight of a substance expressed in grams is known as a __________.

<p>mole</p> Signup and view all the answers

Match the following actions with their appropriate guidelines:

<p>Wash spills immediately = Safety procedure after a chemical spill Handle hot equipment with bare hands = Unsafe practice Use a lab coat = Required protective clothing Read chemical labels = To ensure correct reagent use</p> Signup and view all the answers

Which of the following should NOT be done when working in the lab?

<p>Use broken glassware (A)</p> Signup and view all the answers

It is safe to heat or mix substances near your face.

<p>False (B)</p> Signup and view all the answers

What should you do if you encounter an unknown chemical?

<p>Never smell or taste it.</p> Signup and view all the answers

What is the primary aim of the Faculty of Pharmacy at BAU?

<p>To provide high-quality pharmacy education and scientific research (D)</p> Signup and view all the answers

Students are allowed to use chemicals from unlabeled bottles.

<p>False (B)</p> Signup and view all the answers

List one main safety rule to follow in the laboratory.

<p>Dress appropriately for the lab.</p> Signup and view all the answers

Always read the label written on the ______ bottles before use.

<p>reagent</p> Signup and view all the answers

What should you do after finishing your work in the laboratory?

<p>Clean your place (C)</p> Signup and view all the answers

Match the following titration types with their descriptions:

<p>Acid-Base Titration = Neutralization of an acid with a base Back Titration = Determining the concentration of a substance by reacting it with a known excess of reagent Complex Formation Titration = Formation of a complex compound during titration Direct Titration of Bases = Titrating a base directly with a standard solution of an acid</p> Signup and view all the answers

Chewing gum is allowed in the laboratory.

<p>False (B)</p> Signup and view all the answers

In case of excess reagents, they must be ______ instead of being returned to the stock bottle.

<p>rejected</p> Signup and view all the answers

What is the primary role of NaCl in the analysis of mixtures of HCl and phosphoric acid?

<p>To suppress hydrolysis and liberate protons (A)</p> Signup and view all the answers

H3BO3 is a strong acid that gets ionized completely in a solution.

<p>False (B)</p> Signup and view all the answers

What indicator is used to determine the total acidity in the mixture of hydrochloric acid and phosphoric acid?

<p>Methyl orange</p> Signup and view all the answers

The reaction of 1 ml of 0.1 N HCl corresponds to __________ grams of Na2B4O7·10 H2O.

<p>0.01907</p> Signup and view all the answers

Match the acid with its type:

<p>HCl = Strong acid H3PO4 = Triprotic acid H3BO3 = Weak acid Na2B4O7·10H2O = Boric acid precursor</p> Signup and view all the answers

What happens first in the analysis of a mixture of borax and boric acid?

<p>Titration with standard acid (B)</p> Signup and view all the answers

The overall reaction of borax in solution may be considered as fully neutralized boric acid.

<p>False (B)</p> Signup and view all the answers

What is the purpose of adding glycerol during the titration of boric acid?

<p>To increase the acidic strength for titration</p> Signup and view all the answers

What is the first step to determine the total alkalinity of a mixture of Borax and Sodium Carbonate?

<p>Add known excess of standard acid and boil off CO2 (A)</p> Signup and view all the answers

Barium chloride must be filtered off after precipitating carbonate in Winkler's Method.

<p>False (B)</p> Signup and view all the answers

What indicator is used to titrate the hydroxide content in Winkler's Method?

<p>ph.ph. indicator</p> Signup and view all the answers

1 ml of 0.1 N NaOH corresponds to _____ grams of NaHCO3.

<p>0.0084</p> Signup and view all the answers

Match the following compounds with their corresponding weight in grams for 1 ml of 0.1 N HCl:

<p>Na2CO3 = 0.0053 gm NaOH = 0.00401 gm</p> Signup and view all the answers

In the Two Indicators Method, which portion is determined by titrating with standard acid?

<p>All hydroxide and half carbonate (B)</p> Signup and view all the answers

Borax content can be determined by back titrating the residual unused acid with a standard alkali hydroxide solution.

<p>True (A)</p> Signup and view all the answers

The weight of Borax is calculated using the formula W/V to determine % __________.

<p>W/V</p> Signup and view all the answers

What is the purpose of adding the eosin indicator in the determination of KBr?

<p>To visually indicate the endpoint (B)</p> Signup and view all the answers

The Fajans method uses a nitric acid solution to determine chloride ions.

<p>False (B)</p> Signup and view all the answers

What is used to determine excess silver nitrate in the Volhard method?

<p>Standard thiocyanate</p> Signup and view all the answers

1 ml of 0.05N AgNO₃ = ______ g NaCl

<p>0.002925</p> Signup and view all the answers

Match the following compounds with their respective calculations:

<p>NaCl = 0.002925 g in 1 ml N/20 Ag NO₃ KBr = 0.00595 g in 1 ml 0.05N Ag NO₃ KI = 0.0083 g in 1 ml 0.05N Ag NO₃ HCl = Standardized by Volhard method</p> Signup and view all the answers

Which step is NOT part of the procedure for bromide determination using the Fajans method?

<p>Titrate with NaOH (C)</p> Signup and view all the answers

The process for determining iodide using the Fajans method is the same as that for chloride.

<p>True (A)</p> Signup and view all the answers

What additional component is added in the procedure for HCl detection using the Volhard method?

<p>Concentrated nitric acid</p> Signup and view all the answers

What is the purpose of adding ferric alum in the titration with ammonium thiocyanate?

<p>To act as an indicator for endpoint detection. (B)</p> Signup and view all the answers

In the determination of magnesium, the pH of the solution is maintained at 10.

<p>True (A)</p> Signup and view all the answers

What is the indicator used in the complexometric titration for magnesium?

<p>Eriochrome black T</p> Signup and view all the answers

1 ml M/20 Na2 H2Y = ______ g MgSO4.

<p>0.0060185</p> Signup and view all the answers

Match the following ions with their titration method:

<p>Chloride = Mercurimetric Titration Magnesium = Complexometric Titration Calcium = Complexometric Titration Sodium = Not mentioned</p> Signup and view all the answers

Which of the following solutions is used to prepare the filter paper after washing the precipitate?

<p>Distilled water (D)</p> Signup and view all the answers

The first excess of mercuric ions produces a red color in the supernatant layer.

<p>False (B)</p> Signup and view all the answers

To determine the percentage of NaCl, you need to titrate with ______ solution.

<p>mercuric nitrate</p> Signup and view all the answers

Flashcards

Titrimetric Analysis

A type of chemical analysis that relies on accurately measuring the volume of a solution of known concentration needed to react completely with a substance of unknown quantity

Standardization of Solutions

The process of determining the exact concentration of a solution. It involves reacting a known volume of the solution with a standard solution of known concentration.

Acid-Base Titration

A type of titration where the reaction involves the transfer of protons (H+) between an acid and a base. The endpoint is usually determined using a pH indicator.

Direct Titration

Titration where a known volume of a solution of known concentration (titrant) is directly added to a solution of an analyte of unknown concentration until the reaction is complete.

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Back Titration

Titration used to determine the concentration of a substance that doesn't react directly with the titrant. It involves adding an excess of the titrant, then titrating the excess with a second standard solution.

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Back Titration with Blank Determination

A back titration technique where a known volume of a reagent is added to a blank solution, which does not contain the analyte. The reaction is then performed on the analyte solution and the difference between the two volumes is used to determine the concentration of the analyte.

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Complex Formation Titration

A type of titration that involves the formation of a complex between a metal ion and a suitable reagent. The endpoint is determined by the appearance or disappearance of a color change.

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Laboratory Regulations

The guidelines to follow when conducting experiments in a chemistry laboratory. These rules emphasize safety precautions and good lab practices.

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What is a Mole?

The amount of a substance in grams that corresponds to its molecular weight.

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What is Molarity?

The concentration of a solution expressed as the number of moles of solute per liter of solution.

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Why wear a lab coat?

Always wear a lab coat in the lab to protect yourself from chemicals and spills.

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Broken Glassware

Never use broken glassware in the lab as it can be dangerous.

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Unassigned Experiments

Never perform experiments that are not assigned to you, as this can be dangerous and lead to unexpected results.

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Chemical Spills

If you spill any chemicals on yourself, wash the affected area immediately with plenty of water and report it to your instructor.

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Eyes and Hands

Never touch your eyes with your hands in the lab, as your hands are often contaminated with chemicals.

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Waste Disposal

Dispose of used chemicals and broken glassware in the designated containers.

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Total Acidity Titration

The process of neutralizing both the strong acid (HCl) and the first ionizable proton of the weak acid (H3PO4) with standard NaOH using methyl orange as the indicator.

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Titration with NaCl

The process of neutralizing the total HCl contents and the two ionizable protons of H3PO4 with standard NaOH using phenolphthalein as the indicator and adding NaCl to suppress hydrolysis.

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Borax Titration

This titration determines the borax content by neutralizing it with standard acid (HCl) using methyl orange as the indicator.

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Total Boric Acid Titration

The glycerol increases the acidic strength of the boric acid, allowing it to be titrated with standard NaOH using phenolphthalein as the indicator.

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Strong Acid Suppression

A strong acid, like HCl, suppresses the ionization of a weak acid, like H3BO3, in a solution.

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Mixture Titration

The process of adding standard sodium hydroxide solution (NaOH) to a solution containing both HCl and H3BO3, the HCl reacts first, then the glycerol is added to increase the acidity of the H3BO3 which can then be titrated.

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Borax as Half-Neutralized Boric Acid

The analysis relies on the fact that borax in solution behaves as a half-neutralized boric acid, allowing for titration using a standard acid.

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Mixture Analysis

The analysis involves titrating a mixture containing both borax and boric acid, first with standard acid (HCI) to determine the borax content, then the total boric acid content is determined by titration with standard alkali hydroxide (NaOH) after adding glycerol.

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Volhard Method for Chloride Determination

A method for determining the amount of chloride ions in a sample by reacting it with an excess of silver nitrate solution, followed by the removal of silver chloride and titration of the remaining silver nitrate with standard thiocyanate solution.

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Titration

The technique for determining the concentration of a substance by reacting it with a solution of known concentration (titrant) until the reaction is complete.

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Equivalence Point

The point in a titration at which the reaction between the analyte and the titrant is complete. It is usually indicated by a color change of the indicator.

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Indicator

A substance that changes color in the presence of specific chemicals, indicating the endpoint of a titration.

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Equivalence

The amount of a substance that reacts with one mole of another substance.

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Total Alkalinity Determination

A method for determining the total alkalinity of a mixture, where excess standard acid is added and the solution is boiled to remove CO2. The remaining unused acid is then titrated with a standard alkali hydroxide using methyl orange (MO) as an indicator.

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Hydroxide Content Determination

A method for determining the hydroxide content of a mixture, which involves precipitating the carbonate with barium chloride, and then titrating the hydroxide with a standard acid using phenolphthalein (ph.ph.) as an indicator.

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Winkler's Method

A method for determining the amount of sodium carbonate and hydroxide in a mixture, using a two-indicator approach. In the first step, the total alkalinity is determined using methyl orange as an indicator. In the second step, the hydroxide content is determined using phenolphthalein after precipitating the carbonate with barium chloride.

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Two Indicators Method

A method for determining the concentrations of sodium hydroxide and sodium carbonate in a mixture, using two indicators. It involves titrating the mixture with standard acid, first with phenolphthalein (ph.ph.) to determine the hydroxide and half the carbonate, and then with methyl orange (MO) to measure the total alkalinity.

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Total Alkalinity Determination (Method I)

A method for determining the total alkalinity of a mixture of alkali hydroxide and carbonate, using a standard acid and methyl orange (MO) as an indicator.

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Hydroxide Content Determination (Method I)

A method for determining the hydroxide content of a mixture of alkali hydroxide and carbonate, where the carbonate is first precipitated with barium chloride, and then the hydroxide is titrated with a standard acid using phenolphthalein (ph.ph.) as an indicator.

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Bicarbonate Content Determination

A method for determining the bicarbonate content of a mixture, where the carbonate is first precipitated using barium chloride. The excess unused alkali hydroxide is then titrated with standard acid using phenolphthalein (ph.ph.)

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Total Alkalinity Determination (Method II)

A method for determining the total alkalinity of a mixture of alkali carbonate and bicarbonate, using standard acid and methyl orange (MO) as an indicator.

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Mercurimetric Titration

A titration method used specifically for determining the concentration of chloride ions (Cl-) in a solution using a standard solution of mercuric nitrate (Hg(NO3)2).

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Complexometric Titration

A titration method that relies on the formation of a complex between metal ions and a chelating agent, typically EDTA. It involves adding a known amount of the chelating agent to a solution containing the metal ions.

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Study Notes

Pharmaceutical Analytical Chemistry II Lab Manual (PHAR 270)

  • Course: Pharmaceutical Analytical Chemistry II (PHAR 270)
  • Lab Manual: Fall 2024-2025
  • Mission Statement (Faculty of Pharmacy BAU): The faculty is focused on providing high-quality pharmacy education and conducting scientific research to produce competent pharmacists ready to advance the field nationally and internationally. They emphasize the importance of professional ethics and collaboration with peers and society. Educational tools, qualified staff, and laboratory facilities support this mission.

Contents

  • Lab policy and General information: Pages 3-4
  • Fundamental Terms in Titrimetric Analysis: Page 6
  • Standardization of Solutions: Page 6
  • Acid-Base titration: Page 6
  • Direct Titration of Bases: Page 20
  • Back Titration: Page 24
  • Back Titration with Blank Determination: Page 31
  • Precipitate: Page 33
  • Complex Formation Titration: Page 35

Laboratory Regulations

  • General Conduct: Students must bring a notebook, wear a lab coat, turn off cell phones, and read reagent labels before use. Never use chemicals from unlabeled bottles or return excess reagents to stock bottles. Follow procedures carefully and avoid touching hot equipment. All chemicals are hazardous; minimize exposure time. No talking or gum chewing. Keep work areas clean.

  • Consequences for Violations: Violations may result in expulsion, grade deductions, or being marked absent.

Lab Policy and General Information

  • Safety: Lab safety is paramount. Appropriate attire, such as long pants and tied hair, must be worn.
  • Conduct: Do not eat, drink, or apply cosmetics in the lab. Any incidents of glass breakage or spills require immediate reporting to instructor. Do not perform experiments or procedures not directly assigned.
  • General Safe Work Practices: Be cautious, avoid touching hot objects, handle chemicals safely and dispose properly of used materials. Be aware of the safe use of all lab equipment.

Important Laboratory Rules & Guidelines

  • Attendance & Participation: Required for a good grade.
  • Assignments: Includes lab reports required for each experiment and presentations of results.
  • Expectations: Students will follow all lab rules.
  • Completing the experiments: Students should follow the schedule. All assigned labs and procedures must be completed accurately to pass the class. Incomplete labs will impact the final grade.

Additional Topics (Based on the extracted pages)

  • Calculations with Molarity: Includes formulas for mole, molarity, and calculating titrations, example problems for calculations.
  • Various Titration Techniques: (Acid-base, back titration, precipitate formation, etc.)
  • Specific Lab Procedures: Detailed steps for various experiments (e.g., standardization solutions, determinations of different substances) are outlined.
  • Laboratory equipment and devices: Illustrations of various laboratory equipment such as burettes, volumetric flasks, graduated pipettes, and more.

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Test your knowledge on laboratory safety rules and practices with this quiz. Questions cover various laboratory protocols, proper handling of chemicals, and essential safety measures. Make sure to review best practices to keep yourself and others safe in the lab.

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