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Questions and Answers
Which of the following is a safety measure for laboratory work?
It is safe to wear synthetic fibers in the laboratory.
False
What is the purpose of using a centrifuge?
To separate fluids based on density.
What should you do before starting a centrifuge?
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What does a pH meter measure?
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You should cover your ______ when dealing with incendiary materials.
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What is the first step to use a centrifuge machine?
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Study Notes
Laboratory Safety
- Wear a lab coat and safety glasses at all times.
- Hair must be covered when handling flammable materials.
- Use gloves and protective gear based on experiment requirements.
- Avoid sandals or open-toed shoes to minimize skin exposure to chemicals.
- Clothing made of synthetic fibers should be avoided due to high flammability risks.
- Protective glasses are essential to shield eyes from chemicals and gases.
- Jewelry and adornments, such as chains or rings, should not be worn during lab activities to prevent accidents.
Writing a Laboratory Report
- Introduction: State the experiment's name and its purpose.
- Method: Document the experimental steps in the past tense, closely following the practical manual.
- Discussion and Conclusion: Analyze results thoughtfully without merely repeating them. Formulate relevant questions in the introduction to guide the discussion. Ensure the discussion is engaging and has a clear purpose.
- Data Presentation: Use tables or graphical forms for presenting results, ensuring they are numbered, titled, and include standardized concentrations.
Equipment Used in Enzyme Laboratory
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Centrifuge:
- Separates fluids based on density by spinning at high speeds.
- Commonly used to purify cells, organelles, viruses, proteins, and nucleic acids in various research types.
- Available in different types, including large floor models and micro-centrifuges.
Operating a Centrifuge
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Insert test tube samples into designated portals.
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Add water-filled test tubes as needed for balance.
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Secure the lid and select appropriate settings.
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Start the centrifuge and await the completion of the cycle.
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pH Meter:
- Measures the concentration of H+ ions in a solution, indicating its acidity or alkalinity.
- The meter needs to be calibrated; immerse the electrode in distilled water before transitioning to a buffer solution (pH = 7).
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Description
This quiz covers essential safety protocols to follow in a laboratory setting, including attire and protective gear. It also emphasizes the structure and components of writing an effective laboratory report, guiding students through the introduction, method, discussion, and conclusion sections.
