Laboratory Rules and Safety Quiz
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What is Pharmacognosy?

Pharmacognosy is the study of medicinal drugs originating from biological sources, covering aspects like identification, preparation, content, trade, storage, usage, and control.

What are the main parts of the Pharmacognosy-1 lab?

The Pharmacognosy-1 lab involves practical work divided into two main parts: microscopic examination of drugs and phytochemical analysis.

What is a light microscope and what are its main parts?

A light microscope is a tool for examining small structures like plant tissues and cells. It's primarily composed of mechanical and optical components.

What's the difference between the mechanical and optical parts of a microscope?

The mechanical parts provide the structural foundation and support, while the optical parts focus and magnify the image.

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How is magnification calculated in a microscope?

The magnification power of a microscope is calculated by multiplying the magnification of the ocular (eyepiece) lens by the magnification of the objective lens.

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What precautions should you take while using a microscope?

When using a microscope, ensure a stable surface, keep it away from the edge, remove clutter, and avoid crushing cables.

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What is starch?

Starch is a carbohydrate composed of numerous glucose units linked by glycosidic bonds, serving as a reserve energy source for plants.

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What are the two types of starch in plants?

Starch is produced in leaves through photosynthesis as 'assimilation starch' and stored in various plant parts as 'reserve starch' to balance osmotic pressure.

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What are the properties of starch?

Starch is a tasteless and odourless powder that is partially soluble in water and insoluble in alcohol, used in food for thickening and in pharmacy as a filler.

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Where is starch stored in plants?

Starch is stored as a reserve within leucoplasts in plant tissues like bark, root, rhizome, tuber, and seed.

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What are the hilum and starch circles in a starch grain?

The hilum is the starting point of starch formation in a starch grain, appearing as a point, and the layers around it are called starch circles.

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What are the two types of starch grains based on hilum location?

Starch grains can either have a central hilum (centric) or a hilum at the edge (eccentric).

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What are the two types of starch based on structure?

Simple starch consists of a single starch granule within a leucoplast, while compound starch has multiple hilums and granules.

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What is the epidermis?

The epidermis is a protective tissue covering the outer surface of plants, usually single-layered but occasionally multi-layered.

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What is parenchyma?

Parenchyma is a living tissue composed of thin-walled cells involved in various functions like photosynthesis, respiration, secretion, ventilation, and storage.

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What is cork tissue?

Cork tissue is a protective, non-living tissue formed in the outer bark, with dark-colored, deepened cell walls and varying thickness.

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What is sclerenchyma?

Sclerenchyma is a supportive tissue with thick secondary walls containing lignin, providing mechanical resistance to bending and twisting.

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What are stone cells?

Stone cells are cells with thickened walls due to lignin accumulation, known for their rigidity and often found as idioblasts.

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What is conduction tissue?

Conduction tissue, composed of xylem and phloem, transports water, nutrients, and assimilation products throughout the plant.

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What are stomata?

Stomata are pores on the epidermis that allow gas exchange between the plant and its environment, regulated by guard cells.

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What are trichomes?

Trichomes are outgrowths from epidermal cells that can be either covering (protective) or glandular (secretory).

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What are crystals in plants?

Crystals are mineral products of plant metabolism, mainly calcium oxalate, often found in parenchyma cells and classified as simple or compound.

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What are the different types of plant crystals?

Simple crystals include raphides, crystal sand, and single crystals, while compound crystals include twin crystals, druses, and simple crystal arrays.

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What is a flower?

Flowers (Flores) are the reproductive organs of angiosperm plants, containing sepals, petals, stamens, and pistils.

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What is a leaf?

Leaves (Folia) are the main organs for photosynthesis and transpiration, consisting of epidermis, mesophyll, and vascular bundles.

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What are covering trichomes?

Covering trichomes are outgrowths from epidermal cells that provide protection and can be single-celled or multicelled.

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What are glandular trichomes?

Glandular trichomes are epidermal cells that secrete substances, consisting of a stem and head with different structures.

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What is a fruit?

Fruits (Fructus) are developed ovaries that contain seeds, typically consisting of three layers: exocarp, mesocarp, and endocarp.

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What is a seed?

Seeds (Semen) are formed from fertilized ovules, containing an embryo and nutrient tissue (endosperm) enclosed by a testa (seed coat).

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What are flavonoids?

Flavonoids are chromone derivatives, primarily found in plants as pigments, responsible for the colour of flowers, leaves, and fruits.

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What are anthocyanidins?

Anthocyanidins are pigments with a benzopyrylium structure, forming anthocyanins when combined with oses.

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What are anthracene derivative heterosides?

Anthracene derivative heterosides are compounds containing an anthracene derivative as an aglycone, often possessing cathartic properties.

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What are coumarin derivative heterosides?

Coumarin derivative heterosides have a coumarin structure as an aglycone and often exhibit fluorescent properties.

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What are saponosides?

Saponosides are heterosides that create persistent foam when shaken in water, having haemolytic ability and being classified as steroidal or triterpenoid.

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What are tannins?

Tannins are polyphenolic compounds with astringent properties, found in plants, often used for leather tanning and classified as hydrolysable or condensed.

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What are cardioactive heterosides?

Cardioactive heterosides are compounds affecting heart function, with aglycones like cardenolides (23 carbons) and bufadienolides (24 carbons).

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Study Notes

Laboratory Rules and Safety

  • Timeliness: Arrive on time.

  • Attire: Wear a buttoned lab coat and closed-toe shoes. Avoid dangling jewelry.

  • Food and Drink: Eating, drinking, and using lab materials for these purposes is forbidden. Chewing gum is also not allowed.

  • Safety Precautions: Check labels on chemical containers carefully and do not deform them. Chemical substances should not be touched with bare hands. Leave the laboratory clean. Experiment reports must be written legibly.

  • Preliminary Work: Complete all preliminary steps before entering the lab. Only perform the experiment as instructed.

  • Equipment: Do not use any equipment unless instructed to do so.

  • Discretion: Do not run or joke in the laboratory.

  • Protection: Wear safety goggles and gloves when necessary. Clean equipment after use and clean the workspace. Wear closed-toe shoes.

  • Assistance: Ask for assistance when needed.

  • Cleanup: Keep the area clean and wash hands.

  • Specific Handling: Heated materials should be handled with wooden tongs. Turn off burners or heaters when not in use. Close and unload precision scales when not in use. Do not spill chemicals on the scale, and clean up spills immediately. Do not reuse spatulas without cleaning them first. Keep the fume hood closed when working with acids or alkalies.

  • Spills: Clean up spills immediately.

  • Waste: Dispose of chemicals and other materials properly.

  • First Aid: Follow first aid rules in case of splashing of chemicals on skin or eyes. Do not apply ointments/sprays. Do not remove clothes from burns. Do not touch the injury.

  • Eye Irritation: Protect the uninjured eye. Wash the affected eye with water for at least 15 minutes. Cover both eyes with a clean dressing. Contact health institutions.

  • Fire: Do not run if your clothes catch fire. Extinguish the flame appropriately and get help. Report any fires immediately.

  • Cuts/Bleeding: Clean the wound immediately and apply pressure, following the severity of the injury.

  • Ingesting Chemicals: If a person ingests a chemical, administer water if conscious and alert. If vomiting is observed, stop giving liquid. Transport the exposed person to a health facility immediately.

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Farmakognozi Lab PDF

Description

Test your knowledge of essential laboratory rules and safety protocols. This quiz covers important aspects of laboratory attire, safe conduct, and proper equipment use. Ensure you are well-prepared to maintain a safe and efficient lab environment.

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