Laboratory Diagnosis of Staphylococci 2022
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Questions and Answers

What temperature is the test tube incubated at for the coagulase testing of staphylococcus?

  • 30°C
  • 37°C (correct)
  • 40°C
  • 25°C

    • If no clotting is observed in the test tube after incubation at 37°C for an hour, what should be done next?

  • Incubate for another hour at 45°C
  • Examine at 30-minute intervals for up to 6 hours (correct)
  • Add more test organism culture
  • Examine the tube under a microscope

    • Why should citrated blood not be used for the coagulase test?

  • It inhibits clot formation
  • It causes false-negative results
  • It can lead to false-positive results (correct)
  • It accelerates the clotting process

    • Can coagulase testing be performed using growth from mannitol salt agar?

    <p>No, it cannot be performed from growth on mannitol salt agar</p> Signup and view all the answers

    How is a DNase test spot inoculation performed?

    <p>Touch a colony of the organism with a loop and inoculate onto a small area</p> Signup and view all the answers

    What should be done after flooding a DNase test agar plate with 1N Hydrochloric Acid?

    <p>Decant excess hydrochloric acid</p> Signup and view all the answers

    What happens if Coagulase testing is performed with citrated blood?

    <p>It causes false-positive results</p> Signup and view all the answers

    Which agar type should not be used for Coagulase testing?

    <p><strong>Mannitol Salt Agar</strong></p> Signup and view all the answers

    What property of Staphylococcus aureus contributes to high infection rates and persistence despite treatment?

    <p>Producing biofilms</p> Signup and view all the answers

    Which of the following is a systemic illness associated with Staphylococcus aureus infections?

    <p>Toxic shock syndrome</p> Signup and view all the answers

    What plays a role in determining the treatment for Staphylococcus aureus infections?

    <p>Antibiotic resistance patterns and co-infection rates</p> Signup and view all the answers

    How does Staphylococcus aureus evade host immune responses?

    <p>By producing biofilms</p> Signup and view all the answers

    Which of the following is NOT a factor in the pathogenesis of Staphylococcus aureus infections?

    <p>Producing antibiotics</p> Signup and view all the answers

    What is the function of coagulase enzymes produced by Staphylococcus aureus?

    <p>Help the bacteria evade the human immune response</p> Signup and view all the answers

    Which toxin produced by Staphylococcus aureus disrupts the immune system by causing massive activation of host immune cells?

    <p>Toxic shock syndrome toxin-1 (TSST-1)</p> Signup and view all the answers

    In addition to causing skin and soft tissue infections, what else can Staphylococcus aureus infections lead to?

    <p>Systemic illnesses</p> Signup and view all the answers

    What role do exotoxins produced by Staphylococcus aureus play in causing diseases?

    <p>Activate host immune cells</p> Signup and view all the answers

    Where can Staphylococcus aureus be found besides infecting humans?

    <p>In soil, wildlife animals, and foodstuffs</p> Signup and view all the answers

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