Labelling Protocols in the Laboratory

Which samples need to be labelled with the name of the project they belong to?

Only customer samples
All samples (correct)
Only solid food samples
Only processed samples

Where should the samples be stored?

In a box
In the fridge (correct)
In the biosafety cabinet
On the shelves

What information should be included in the label of processed samples?

Project name, 1:10 dilution ratio, and date
Contents, name of the experiment, date, and initials (correct)
ISO guidelines and experiment date
Customer name, type of sample, and initials

Where can ISO standards be found?

OneDrive > Guildford R&D > Standards (B) Signup and view all the answers

What should be used to clean the working surface area in the biosafety cabinet?

Klericide and Virkon (B) Signup and view all the answers

Why is a 1 in 10 dilution prepared for most solid food samples?

To have a viable liquid to use as a neat sample (D) Signup and view all the answers

What are ISO standards?

Guides with internationally agreed information and guidelines (B) Signup and view all the answers

Where should sample handling, especially customer samples, take place?

In the biosafety cabinet (D) Signup and view all the answers

Which step in the Gram stain procedure allows the sample to take up stains more readily?

Heat fixing (D) Signup and view all the answers

What should be done after the smear has air-dried during the heat fixing process?

Place the slide on a hot heat plate (A) Signup and view all the answers

What color will a Gram-positive smear appear after the Gram stain procedure?

Purple (D) Signup and view all the answers

What is the purpose of decolorizing with ethyl alcohol in the Gram stain procedure?

To remove the crystal violet stain from Gram-negative bacteria (C) Signup and view all the answers

Which of the following is a type of API strip used for bacterial identification?

RiboPrinter (B) Signup and view all the answers

What is the purpose of agglutination kits in bacterial identification?

To detect specific antigens in the organism (C) Signup and view all the answers

Which method can be used to confirm the genus of a suspect colony?

BAX kits (C) Signup and view all the answers

What is the purpose of the RiboPrinter in bacterial identification?

To provide a genus and species identification (B) Signup and view all the answers

Which of the following tests have been verified to work with BAX kits?

InSite Salmonella and InSite Listeria (C) Signup and view all the answers

Which of the following is the correct method for sampling solid food samples?

Weigh out the volume of sample needed on the side without the filter attached. (C) Signup and view all the answers

Which of the following surfaces can be sampled for testing?

Plastic, silicone, concrete, and ceramic (A) Signup and view all the answers

What should be used to sterilize surfaces that cannot be autoclaved?

Virkon (D) Signup and view all the answers

What is the purpose of a filtered stomacher bag in the sample processing procedure?

To avoid blocking pipettes (C) Signup and view all the answers

What is the next step after isolating an organism on media outlined in ISO?

Restreak the colony on non-selective agar (A) Signup and view all the answers

What is the purpose of the oxidase test in bacterial identification?

To test for the presence of a specific enzyme (C) Signup and view all the answers

What is the purpose of the catalase test in bacterial identification?

To test for the presence of a specific enzyme (A) Signup and view all the answers

What is the purpose of the Gram staining procedure?

To determine if a bacterium is Gram-positive or Gram-negative (D) Signup and view all the answers