Lab Safety Basics in Biology

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Questions and Answers

What is the base unit for measuring mass in the metric system?

  • Gram (correct)
  • Liter
  • Celsius
  • Meter

Which measuring device is commonly used for measuring volume in liters?

  • Thermometer
  • Graduated cylinder (correct)
  • Ruler
  • Electronic balance

Which prefix in the metric system represents one-thousandth of a unit?

  • Deci-
  • Milli- (correct)
  • Cent-
  • Kilo-

What is the standard base unit for measuring length in the metric system?

<p>Meter (B)</p> Signup and view all the answers

What should you avoid when measuring with a ruler to ensure accuracy?

<p>Starting from the end of the ruler (C)</p> Signup and view all the answers

Which of the following prefixes indicates a quantity of ten?

<p>Deca- (D)</p> Signup and view all the answers

Which temperature unit is commonly used in scientific measurements?

<p>Degrees Celsius (A)</p> Signup and view all the answers

What is the unit symbol for mass in the metric system?

<p>g (A)</p> Signup and view all the answers

What should you do if chemicals splash onto your skin or into your eyes during a lab?

<p>Use the safety shower or eyewash station and inform the instructor. (A)</p> Signup and view all the answers

Why is it important to know the location of safety equipment like fire extinguishers and eyewash stations before starting a lab?

<p>In case of an emergency, quick access can be life-saving. (C)</p> Signup and view all the answers

What precautions should you take when handling chemicals in the lab?

<p>Wear safety goggles and rubber gloves. (A)</p> Signup and view all the answers

How should sharp objects or biohazardous waste materials be disposed of in the lab?

<p>Place them in puncture-resistant containers. (D)</p> Signup and view all the answers

Why is it important to never eat, drink, or apply cosmetics in the laboratory?

<p>Contaminants can lead to serious health risks. (A)</p> Signup and view all the answers

What is the primary focus of biology as a field of study?

<p>The systematic study of living phenomena. (C)</p> Signup and view all the answers

Which of the following describes the role of biologists?

<p>To observe, ask questions, and design experiments to explain observations. (A)</p> Signup and view all the answers

What should you do immediately after a spill occurs in the laboratory?

<p>Ask your instructor about the proper cleanup procedure. (A)</p> Signup and view all the answers

What should you do if you are unsure about any part of the laboratory investigation?

<p>Ask your lab instructor for assistance (B)</p> Signup and view all the answers

Which of the following is NOT a general laboratory safety guideline?

<p>Use your phone for taking notes (B)</p> Signup and view all the answers

What should be done immediately if you break a glass item in the lab?

<p>Notify the lab's instructor for a clean-up procedure (D)</p> Signup and view all the answers

Why is it important to remove large jewelry and tie back long hair in the lab?

<p>To reduce the risk of accidents during experiments (B)</p> Signup and view all the answers

What should you do with materials that have come into contact with biohazardous waste?

<p>Dispose of them in the proper biohazardous waste container (D)</p> Signup and view all the answers

Which safety equipment should be worn when instructed during lab activities?

<p>A lab coat, gloves, and safety goggles (B)</p> Signup and view all the answers

How should you prepare before arriving at the laboratory?

<p>Read through the exercises to familiarize yourself with them (B)</p> Signup and view all the answers

What requirement must be fulfilled before handling laboratory equipment?

<p>You must have specific permission from the lab instructor (C)</p> Signup and view all the answers

What instrument is most commonly used to measure mass?

<p>Triple-beam balance (B)</p> Signup and view all the answers

How should the meniscus be read when measuring the volume of a liquid?

<p>At the bottom of the curve of the meniscus (A)</p> Signup and view all the answers

Which method is appropriate for calculating the volume of a wooden block?

<p>Length × width × depth (C)</p> Signup and view all the answers

What is the freezing point of water in Celsius?

<p>0 degrees Celsius (A)</p> Signup and view all the answers

When measuring with a ruler, how should you handle significant figures?

<p>Record all known digits plus the first uncertain digit (C)</p> Signup and view all the answers

What is the primary use of a graduated cylinder?

<p>Measuring the volume of liquids (C)</p> Signup and view all the answers

What is a correct conversion formula between Celsius and Fahrenheit?

<p>°F = °C × 1.8 + 32 (B)</p> Signup and view all the answers

Which is NOT a tool used for measuring weight or mass?

<p>Graduated cylinder (C)</p> Signup and view all the answers

Which variable is considered independent in an experiment testing bacterial growth?

<p>Temperature at which the experiment is conducted (B)</p> Signup and view all the answers

What is the dependent variable in the context of bacterial growth experiments?

<p>The percentage of bacterial growth observed (A)</p> Signup and view all the answers

Which of the following would be a controlled variable in an experiment on bacterial growth?

<p>The amount of Substance A used (C)</p> Signup and view all the answers

What aspect of the experiment could be altered to improve reliability of results?

<p>Controlling other environmental factors (D)</p> Signup and view all the answers

When conducting a control test, what is being measured?

<p>The baseline growth without the experimental variable (A)</p> Signup and view all the answers

If you observe that the dish with the highest temperature had the least bacterial growth, what can be inferred?

<p>Higher temperatures may inhibit bacterial growth (B)</p> Signup and view all the answers

During the experiment, why is it important to estimate bacterial growth as a percentage?

<p>It allows for a standardized comparison between tests (A)</p> Signup and view all the answers

Which of the following statements about antibacterial agents is correct in an experimental context?

<p>The effects of different agents can be compared only in a consistent experiment setup (D)</p> Signup and view all the answers

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Study Notes

Lab Safety

  • Students should familiarize themselves with lab safety rules and the location of safety equipment.
  • Before entering the lab, students must turn off their cell phones, tie back long hair, remove large jewelry, and wear appropriate attire (closed-toe shoes and lab coat).
  • Students must carefully read and follow all instructions provided by the instructor and seek clarification if needed.
  • Always use proper techniques and protection when handling chemicals, and ensure that any spills are reported to the instructor.
  • Students should always wear gloves and safety goggles when handling chemicals.
  • Never directly smell or mouth pipette any chemicals.
  • Dispose of all used sharp objects and biohazardous waste materials in designated containers.
  • Wash hands before and after lab investigations.
  • Be aware of the locations of safety equipment such as fire extinguishers, fire blankets, first-aid kits, safety showers, and eyewash stations.

Introduction to Biology and The Scientific Method

  • Biology involves observation, experimentation, and explanation of living phenomena.
  • Students are tasked with designing an experiment to explore the effects of antibacterial agents or temperature on bacterial growth.
  • Key steps in the scientific method include formulating a question, developing a hypothesis, identifying independent and dependent variables, and controlling variables.
  • The independent variable is the factor being manipulated or changed in the experiment, while the dependent variable is the outcome being measured.
  • Controlled variables are kept constant to ensure a fair comparison.

Scientific Measurements

  • The metric system is used for scientific measurements, using base units like meters for length, grams for weight/mass, and liters for volume.
  • Length is measured using rulers, focusing on precision and recording all known digits and the first uncertain digit.
  • Weight/mass is measured using balances, such as triple-beam balances or top-loading scales.
  • Liquid volume is measured using graduated cylinders or pipettes, reading the volume at the bottom of the meniscus.
  • Solid object volume can be calculated using formulas, for example, volume of a wooden block = length x width x depth.
  • Temperature is measured using scientific thermometers calibrated in Celsius (°C), with water boiling at 100 °C and freezing at 0 °C.
  • Formulas exist to convert between Celsius and Fahrenheit scales.
  • When taking measurements, students should use significant figures, recording all known digits and the first uncertain digit.

Taking Measurements

  • Students are tasked with measuring the length and width of different geometric shapes using a metric ruler.
  • Measurements must be recorded using significant figures.
  • The area of the geometric shapes needs to be calculated using the measurements obtained, and the formula area = width x length.

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