Lab Safety and Procedures Quiz

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Questions and Answers

What procedure is used to separate substances in a mixture?

Acceleration of gravitational force based on density (correct)

Evaporation based on boiling points

Centifugation to separate solid from liquid

Filtration using a membrane

Which hazard symbol indicates a substance is flammable?

Biohazard

Droplet

Skull and Crossbones

Flaming (correct)

Which of the following describes the correct manner to read a pipette?

Read the volume at the bottom of the meniscus (correct)

Look at the average of both meniscus levels

Use the highest digital reading for accuracy

Measure from the top of the meniscus

What does the skull and crossbones hazard symbol indicate?

Poisonous or toxic substances Signup and view all the answers

What type of hazard is represented by the tree and fish symbol?

Environmental hazard Signup and view all the answers

What is the primary purpose of incubating samples in a water bath?

To maintain a constant temperature for optimal reactions Signup and view all the answers

Which of the following options is NOT a characteristic associated with the 'oxidizer' hazard symbol?

Causes corrosion Signup and view all the answers

When separating dense substances from lighter ones, which method is most effective?

Utilizing a centrifuge to create density gradients Signup and view all the answers

What is the primary purpose of a volumetric flask?

To hold a fixed volume of liquids. Signup and view all the answers

Which instrument is specifically designed for the precise delivery of liquids?

Burette Signup and view all the answers

What instrument is specifically designed for measuring precise volumes of fluids?

Pipette Signup and view all the answers

Which instrument is primarily used to maintain the organization of multiple test tubes?

Test tube rack Signup and view all the answers

Which piece of equipment is used for separating components of a sample based on density differences?

Centrifuge Signup and view all the answers

What is the primary purpose of a volumetric flask in a laboratory?

To prepare solutions in precise amounts Signup and view all the answers

What role does detergent play in the DNA isolation process?

To capture lipids and proteins, freeing the DNA Signup and view all the answers

Why is alcohol used during DNA isolation?

To precipitate DNA, making it visible Signup and view all the answers

What is the primary function of NaCl in the DNA isolation process?

To precipitate DNA and neutralize its charge Signup and view all the answers

How does meat tenderizer contribute to DNA isolation?

By digesting proteins that bind to DNA Signup and view all the answers

What is the correct relationship between pH and [H+] concentration?

pH = -log[H+] Signup and view all the answers

Which formula correctly calculates molarity (M)?

M = Moles of solute / volume of solution Signup and view all the answers

What does a DNA purity ratio higher than 2 indicate?

Contamination with RNA Signup and view all the answers

Study Notes

Lab Equipment and Uses

Test Tube: Used to hold and test small amounts of chemicals.
Test Tube Rack: Holds test tubes.
Test Tube Holder: Holds test tubes.
Dropper: Adds small volumes of liquids.
Pipette: Delivers small volumes of liquids (millimeters).
Micropipette: Delivers small volumes of liquids (micrometers).
Burette: Accurate delivery of liquid volumes.
Round Flask: Used to hold and heat liquids.
Volumetric Flask: Holds a fixed volume of liquid.
Erlenmeyer Flask: Used to hold, heat, and mix liquids; reduces evaporation.
Funnel: Reduces liquid evaporation in small containers; used for pouring and measuring liquids.
Beaker: Pour and measure liquids.
Balance: Measures mass (weight).
Hotplate/Magnetic Stirrer: Heats materials; mixes solutions with a magnetic stirrer.
pH-meter: Measures pH of aqueous solutions.
Spectrometer: Measures light intensity at specific wavelengths, to identify the concentration of a compound in an aqueous solution.
Microscope: Used to look at microscopic specimens.
Centrifuge: Separates substances of different densities in a mixture by accelerating gravitational force.
Water Bath: Incubates samples in water at a constant temperature.

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Description

Test your knowledge on laboratory safety symbols and methods of separation for mixtures. This quiz covers the key hazard symbols, proper lab procedures, and the use of equipment like pipettes and water baths. Challenge yourself and ensure you're prepared for safe science practices!