Lab 1: Bacterial Genetics Principles
23 Questions
0 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

Variations in bacteria that involve alteration of the DNA macromolecule are designated as temporary variations.

False

Phenotypic variations in bacteria disappear as soon as the environmental changes that brought them about disappear.

True

Mutations can be caused by UV light in sunlight or chemicals in the soil.

True

Permanent variations in bacteria are always caused by mutations.

<p>False</p> Signup and view all the answers

Induced mutations are the result of the bacterium's exposure to a mutagen, which is a physical or chemical agent.

<p>True</p> Signup and view all the answers

Urea, phenol, and various acids can be mutagens.

<p>True</p> Signup and view all the answers

The antibiotic streptomycin is only present in the top layer of the gradient plate.

<p>True</p> Signup and view all the answers

Staphylococcus aureus is resistant to streptomycin.

<p>False</p> Signup and view all the answers

The gradient plate is prepared by pouring melted tryptic soy agar into a petri plate placed at an angle.

<p>True</p> Signup and view all the answers

A 24-hour tryptic soy broth culture of Escherichia coli is required for this experiment.

<p>False</p> Signup and view all the answers

Two tryptic soy broth tubes containing $50,\mu\mathrm{g}$ of streptomycin are needed for the experiment.

<p>True</p> Signup and view all the answers

The procedure involves using a spreading rod to spread the bacterial culture on the gradient plate.

<p>True</p> Signup and view all the answers

Ultraviolet light and x-rays are examples of physical agents that can act as mutagens.

<p>True</p> Signup and view all the answers

A point mutation always results in a change in the amino acid sequence of the protein produced.

<p>False</p> Signup and view all the answers

In an inversion mutation, a piece of DNA is replaced by an identical piece in reverse order.

<p>True</p> Signup and view all the answers

A reversion mutation always results in the organism reverting back to its original phenotype.

<p>True</p> Signup and view all the answers

A gradient plate is used to determine the ability of organisms to produce mutants that are resistant to antibiotics.

<p>True</p> Signup and view all the answers

The tryptic soy agar should be labeled with the student's name and date.

<p>True</p> Signup and view all the answers

The tryptic soy agar containing streptomycin should be poured onto the surface of the gradient-agar plate after it has solidified.

<p>True</p> Signup and view all the answers

$0.5$ ml of the Staphylococcus aureus culture should be pipetted onto the agar surface.

<p>False</p> Signup and view all the answers

The plate should be incubated at $37^\circ\mathrm{C}$ for $4$ to $7$ days in an open environment.

<p>False</p> Signup and view all the answers

After incubation, resistant Staphylococcus aureus colonies should be observed in areas of higher streptomycin concentration.

<p>True</p> Signup and view all the answers

Three control tubes should be inoculated with the initial Staphylococcus aureus culture: one without streptomycin, one with $10$ µg, and one with $50$ µg of streptomycin.

<p>True</p> Signup and view all the answers

More Like This

Quiz de Genética Bacteriana y ADN
10 questions
Biology: Bacteria Resistance to Chemical
10 questions
Use Quizgecko on...
Browser
Browser