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Questions and Answers

What is the primary function of RNA polymerase during transcription?

  • To synthesize RNA from a DNA template (correct)
  • To replicate DNA
  • To splice RNA molecules
  • To translate mRNA into proteins
  • What distinguishes eukaryotic transcription from prokaryotic transcription?

  • Eukaryotic transcription involves the use of transcription factors (correct)
  • Eukaryotes do not require RNA polymerase
  • Eukaryotic transcription occurs in the cytoplasm
  • Prokaryotes use only a single RNA polymerase type
  • What is a polycistronic transcript?

  • A specially modified transcript that lacks a 5' cap
  • A transcript that can be translated into multiple proteins (correct)
  • A transcript that requires splicing before translation
  • A transcript encoding a single gene
  • Which of the following correctly describes a consensus sequence?

    <p>A region of DNA that recruits various transcription factors</p> Signup and view all the answers

    During the elongation phase of transcription, which direction is RNA synthesized?

    <p>5' to 3'</p> Signup and view all the answers

    What term describes the opposite orientation of the two strands of DNA?

    <p>Antiparallel</p> Signup and view all the answers

    Which enzyme is responsible for unwinding the DNA helix during replication?

    <p>Helicase</p> Signup and view all the answers

    What is the main difference between leading and lagging strands in DNA replication?

    <p>One is replicated continuously while the other is not.</p> Signup and view all the answers

    Why is an RNA primer necessary in DNA replication?

    <p>It provides a starting point for DNA polymerase to initiate synthesis.</p> Signup and view all the answers

    What are replication forks?

    <p>Structures formed by the unwinding of DNA during replication.</p> Signup and view all the answers

    Which process describes the incorporation of nucleotides into a growing DNA strand?

    <p>Replication</p> Signup and view all the answers

    What is the significance of the AT-rich region in DNA replication initiation?

    <p>It is easier to unwind due to fewer hydrogen bonds.</p> Signup and view all the answers

    In which direction does DNA polymerase synthesize new DNA strands?

    <p>5' to 3'</p> Signup and view all the answers

    Which of the following describes the primary function of DNA polymerase III in bacterial replication?

    <p>It synthesizes new DNA strands.</p> Signup and view all the answers

    What is the role of the origin replication complex (ORC) in eukaryotic DNA replication?

    <p>It licenses replication origins ensuring sites are not duplicated.</p> Signup and view all the answers

    What is a key difference between the leading and lagging strands during DNA replication?

    <p>Leading strand is synthesized continuously, while lagging strand is synthesized in segments.</p> Signup and view all the answers

    What is the primary function of telomeres in eukaryotic cells?

    <p>To protect chromosome ends from degradation.</p> Signup and view all the answers

    Which statement accurately describes the activity of DNA polymerase I in bacterial replication?

    <p>It primarily acts to remove RNA primers.</p> Signup and view all the answers

    How does the eukaryotic helicase MCM2-7 differ from bacterial helicase?

    <p>It comprises multiple subunits instead of a single unit.</p> Signup and view all the answers

    What role does FEN-1 play in DNA replication?

    <p>It removes displaced nucleotides during primer replacement.</p> Signup and view all the answers

    What happens to a cell that lacks telomeres?

    <p>It experiences gradual loss of genetic information upon division.</p> Signup and view all the answers

    Study Notes

    Transcription

    • RNA polymerase synthesizes RNA from a DNA template
    • Eukaryotic transcription occurs in the nucleus, while prokaryotic transcription occurs in the cytoplasm
    • A polycistronic transcript is an mRNA molecule that encodes multiple proteins
    • Consensus sequence: a short sequence of nucleotides that is generally recognized by specific proteins
    • During elongation, RNA is synthesized in the 5' to 3' direction
    • The two strands of DNA run in opposite directions (antiparallel)
    • DNA helicase unwinds the DNA helix during replication

    DNA Replication

    • DNA polymerase adds nucleotides to the growing DNA strand
    • The leading strand is synthesized continuously, while the lagging strand is synthesized discontinuously due to the antiparallel nature of DNA
    • An RNA primer is needed to initiate DNA synthesis because DNA polymerases cannot initiate synthesis de novo
    • Replication forks are Y-shaped structures that form during DNA replication
    • DNA synthesis is the process of adding nucleotides to a growing DNA strand
    • The AT-rich region is involved in the initiation of DNA replication
    • DNA polymerase synthesizes new DNA strands in the 5' to 3' direction
    • DNA polymerase III is the primary enzyme responsible for DNA synthesis in bacterial replication
    • The ORC binds to the origin of replication and recruits other proteins to initiate DNA replication
    • The lagging strand is synthesized discontinuously, while the leading strand is synthesized continuously
    • Telomeres are protective caps at the ends of chromosomes that prevent degradation of DNA
    • DNA polymerase I removes RNA primers and replaces them with DNA
    • MCM2-7 is a eukaryotic helicase that unwinds DNA during replication, while bacterial helicase is a single polypeptide
    • FEN-1 removes RNA primers and Okazaki fragments during DNA replication
    • Cells lacking telomeres experience progressive shortening of their chromosomes, leading to cellular senescence or apoptosis

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