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Questions and Answers

What is the primary biochemical change that occurs in muscle post-mortem?

  • Conversion of muscle to meat (correct)
  • Depletion of lactic acid
  • Increase in ATP production
  • Increase in aerobic metabolism

What occurs when pH decreases too rapidly in meat?

  • Loss of muscle elasticity
  • Denaturation of muscle proteins (correct)
  • Improved water holding capacity
  • Dark coloration of the meat

What is rigor mortis primarily a result of?

  • Excess ATP in muscles
  • High oxygen levels in tissues
  • Lactic acid accumulation
  • Formation of actomyosin (correct)

Which of the following is a consequence of aging meat during rigor resolution?

<p>Alterations in the structure of myofibrils (A)</p> Signup and view all the answers

What happens to ATP levels during rigor mortis?

<p>ATP is depleted (A)</p> Signup and view all the answers

How does pH affect meat color quality if it remains too high?

<p>It turns too dark (D)</p> Signup and view all the answers

What are the main flavor potentiators generated during the aging of meat?

<p>IMP and ammonia (A)</p> Signup and view all the answers

What is a common result of cooking meat while it is still in rigor?

<p>Quite tough texture (D)</p> Signup and view all the answers

What is the primary role of myosin in muscle fibers?

<p>It forms the thick filament proteins necessary for contraction. (B)</p> Signup and view all the answers

How long can raw beef cuts like roasts and steaks be stored in the freezer?

<p>6-12 months (A)</p> Signup and view all the answers

What are the main components of muscle tissue?

<p>Water, protein, and non-protein nitrogen (C)</p> Signup and view all the answers

Which protein constitutes the thin filament in muscle fibers?

<p>Actin (C)</p> Signup and view all the answers

What is the characteristic arrangement called that is formed by thick and thin filaments?

<p>Sarcomere (C)</p> Signup and view all the answers

Which of the following enzymes is used for tenderizing meat?

<p>Papain (D)</p> Signup and view all the answers

What percentage of muscle protein is made up by tropomyosin?

<p>5% (B)</p> Signup and view all the answers

What is the main function of the sarcolemma in muscle fibers?

<p>To transmit signals for muscle contraction (B)</p> Signup and view all the answers

What is a characteristic of cooked sausages?

<p>They must be refrigerated after cooking. (B)</p> Signup and view all the answers

What distinguishes fresh smoked sausages from fresh sausages?

<p>Fresh smoked sausages must be thoroughly cooked before eating. (C)</p> Signup and view all the answers

Which preservation method is primarily used alongside salting and curing?

<p>Drying (C)</p> Signup and view all the answers

What is the primary purpose of curing in meat preservation?

<p>To kill bacteria and prolong shelf life of meat. (C)</p> Signup and view all the answers

How does salting preserve meat?

<p>It draws water out of microbial cells through osmosis. (C)</p> Signup and view all the answers

What is a limitation of the smoking process in meat preservation?

<p>Smoking compounds adhere only to the outer surfaces of meat. (A)</p> Signup and view all the answers

Which of the following best describes dry sausages?

<p>They are fresh sausages that have been dried. (A)</p> Signup and view all the answers

What effect does heating have on meat?

<p>It causes protein coagulation and releases juices. (C)</p> Signup and view all the answers

Flashcards

Tenderizing Meat

Making meat easier to chew and digest using enzymes. These enzymes break down tough proteins, making the meat softer.

Plant Enzymes for Tenderizing

Enzymes like ficin, papain, and bromelain from plants are used to tenderize meat. These enzymes break down proteins, making the meat softer.

Meat Aroma

The smell of meat is influenced by both non-volatile and volatile compounds.

Non-Volatile Flavor Substances

These compounds don't evaporate easily and contribute to the overall taste of the meat.

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Volatile Aroma Constituents

These compounds evaporate easily, giving meat its characteristic aroma.

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Muscle Cell

The basic unit of muscle tissue, responsible for converting chemical energy (ATP) into mechanical energy (movement).

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Sarcolemma

A thin membrane surrounding each muscle fiber. It receives signals from nerve endings, triggering muscle contractions.

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Sarcoplasm

The fluid inside a muscle fiber that surrounds myofibrils. These are protein structures responsible for muscle contraction.

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Cooked Sausage

Sausage made with fresh meat that is fully cooked. It can be eaten immediately or kept refrigerated.

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Fresh Sausage

Sausage made with fresh meat that has not been cured. Must be refrigerated and thoroughly cooked before eating.

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Dry Sausage

Fresh sausage that is dried. Generally eaten cold and keeps for a long time.

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Hot Dog

A fully cooked, cured and/or smoked sausage with soft texture and flavor.

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Drying (Meat Preservation)

An ancient method of food preservation commonly used with salting, curing, and smoking. It should not affect the water holding capacity or aroma of the meat.

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Cooling/Freezing (Meat Preservation)

Freezing meat for prolonged preservation of its freshness. Lengthens shelf life and slows down lipid oxidation.

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Curing (Meat Preservation)

Process of preserving and flavoring meat by adding a combination of salt, sugar, nitrates, and/or nitrites.

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Salting (Meat Preservation)

Adding salt to meat. Salt inhibits the growth of spoilage-causing microorganisms by drawing water out of their cells.

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Tropocollagen

A protein unit that forms collagen, which strengthens muscle tissue. More collagen means tougher meat.

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Gelatin

A soft, jelly-like substance formed by heating collagen. It makes meat tender.

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Adipose Tissue

Fat tissue that stores energy and contributes to meat tenderness and flavor.

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Intramuscular Fat

Fat embedded within muscle tissue, also called marbling. It significantly influences meat flavor and tenderness.

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Post-mortem Changes

A series of biochemical reactions that occur after an animal is slaughtered, transforming muscle into meat.

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Anaerobic Metabolism

The process of energy production in the absence of oxygen. This happens in muscle after an animal is slaughtered, leading to lactic acid buildup and pH drop.

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pH Drop in Meat

A decrease in the acidity of muscle tissue after death, influencing meat quality: faster drop can cause pale & dry meat, while slower drop can cause dark & dry meat.

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IMP (Inosinemonophosphate)

A flavor-enhancing molecule formed during meat aging, contributing to savory flavors.

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Study Notes

Food Chemistry - Meat

  • Meat is animal tissue used as food. It most often refers to skeletal muscle and associated fat. It may also refer to non-muscle organs like lungs, livers, skin, brains, bone marrow, and kidneys.
  • Main sources of meat in the Philippines include pig, goat, carabao, cow, chicken, and horse.

US Per Capita Meat Consumption (1970-1996)

  • Data is presented in a table, showing retail weight figures for different types of meat (beef, veal, pork, lamb, chicken, turkey, fish) from 1970 to 1996.

Heme Iron Sources

  • A table showing milligrams of iron in various meats (3 oz. cooked). Beef (calves liver, sirloin), pork (ground lean, tenderloin, ham), lamb (loin), veal (loin), and chicken (breast) are listed.

Total Fat Content

  • A bar graph illustrates total fat (grams) per 3-ounce serving of various cuts of meat. Meat types include several cuts of Beef, Pork, Lamb and Chicken.

Calories per Serving

  • A bar graph showcasing the calorie count (for 3 ounce serving) across a variety of different cuts of Beef, Pork, Lamb and Chicken. The meat types include various cuts of Beef, Pork, Lamb, and Chicken.

Cholesterol Per Serving

  • A bar graph demonstrating cholesterol content (mg) in different cuts of meat (3 oz serving). Cuts of beef, pork, lamb, and chicken are displayed.

Pork

  • Culinary name for meat from domestic pigs (Sus scrofa). Often refers specifically to fresh meat but can be used as a general term.
  • One of the most commonly consumed meats worldwide. In 2006, nearly 100 million metric tons of pork were consumed globally.

2006 Worldwide Pork Consumption

  • A table detailing pork consumption in different regions. This includes the metric tons (millions), per capita (kg), and region. The data represents a breakdown globally, including totals. Key regions are China, EU25, US, Russia Federation, Japan, and others.

Beef

  • Culinary name for the meat of bovines (domestic cattle).
  • Can be cut into steaks, pot roasts, short ribs, or ground.
  • Blood can be used in blood sausage.

Veal

  • Meat from young calves (usually male).
  • Known for its delicate taste and tender texture.
  • Characteristic odor is sour and aromatic.
  • Best age is 4-14 weeks.
  • Typically hung for 8 days prior to use.

Lamb, Hogget or Mutton

  • Culinary names for the meat of domesticated sheep.
  • Lamb is meat from year-old or younger sheep.
  • Hogget or mutton is meat from older sheep, depending on age and characteristics.
  • Lamb meat comes from animals between one month and one year, averaging 5.5 to 30 kilograms in weight.
  • Highest meat quality is from 2 to 4 year old sheep.

Goat Meat

  • Taste is similar to lamb.
  • Prepared in various ways (stewed, curried, baked, grilled, barbecued, minced, canned).
  • Nutritionally healthier than mutton.
  • Lower in fat and cholesterol, and comparable to chicken.
  • Contains more minerals than chicken
  • Lower in total and saturated fats compared to other meats.

Horse Meat

  • Culinary name for meat cut from a horse.
  • Slightly sweet, tender, low in fat, high in protein.

Sausage

  • Type of food usually consisting of ground meat, animal fat, salt, and spices, and sometimes other ingredients (e.g., herbs).
  • Can be fresh or preserved by curing, drying or smoking.

Sausage Classification

  • Categorization of sausage based on methods. Includes cooked, cooked smoked, fresh, fresh smoked, and dry sausages. Specific processes explain the types of preparations used in sausage-making.

Hot Dog

  • Type of fully cooked, cured and/or smoked moist sausage with a soft, even texture and flavor. Traditionally made from beef, pork, or a combination of both.

Ham

  • Thigh and rump of any slaughtered animal, often strictly the haunch of a pig/boar.

Storage and Preservation of Meat

  • Drying: An ancient food preservation method often combined with salting and curing. Protects against water holding and aroma degradation in meat
  • Cooling/Freezing: Meat freezing preserves freshness over long durations by freezing the meat, while cooling slows down lipid oxidation.
  • Curing: Variety of preservation and flavoring processes for meat. Often uses salt, sugar, and nitrate/nitrite. Commonly coupled with smoking. Increases the product shelf life by eliminating bacterial contamination.
  • Salting: Addition of salt inhibits growth of spoilage-causing microorganisms. Draws water from microbial cells through osmosis.
  • Smoking: Process of flavoring, cooking or preserving food via smoke from burning/smoldering of plant material. Primarily effective in surface application, antimicrobial and antioxidant activity
  • Heating: Causes grayish-brown color, protein coagulation, releasing juices. Reduces the water holding capacity and increase the pH of a meat product.
  • Tenderizing: Process of using plant enzymes (ficin, papain, bromelain) to tenderize meat.

Storage Times

  • Table presenting freezer and refrigerator storage times for beef, lamb, pork, and poultry.

Meat Aroma

  • Raw meat has a weak aroma but heating enhances it.
  • Meat aroma is a complex combination of volatile aroma constituents, non-volatile flavor substances and flavor enhancers.

Meat Composition

  • Water: ~75%
  • Protein: ~18%
  • Lipid: ~3%
  • Non-protein Nitrogen: ~1.6%
  • Carbohydrates: ~1.2%
  • Minerals: ~0.7%
  • Vitamins: Traces

Muscle Cells

  • Unique in converting chemical energy (ATP) to mechanical energy, allowing them to work.
  • Basic unit is the muscle cell, measuring 10-100 µm in diameter and several centimetres long.

Muscle Structure

  • Muscle fibers are organized into bundles, then surrounded by layers of connective tissue (epimysium, perimysium, endomysium).
  • Myofibrils are composed of thin and thick filaments (actin and myosin), creating a light/dark pattern under electron microscopy.

Sarcolemma

  • Thin membrane surrounding each muscle fiber.
  • Site where motor nerve endings terminate, transmitting signals for muscle contraction.

Sarcoplasm

  • Fluid within the muscle fiber, suspending the myofibrils.
  • Contains roughly 2000 myofibrils per muscle fiber.

Myofibrils

  • Protein structures inside muscle fibers comprising thick and thin filaments.
  • Thick filaments are primarily myosin, composing ~45% of muscle protein.
  • Thin filaments are primarily actin, making up ~20-25% of muscle protein.

Thick Filaments

  • Principal protein of the A band is myosin.
  • Myosin molecular weight is ~470,000-480,000 Daltons.
  • Contains ~400 myosin molecules per thick filament

Thin Filaments

  • Core component of the I-band in muscle tissue.
  • Composed predominantly of actin protein. Representing ~20-25% of muscle protein.
  • Actin structure exists in two forms, G-actin and F-actin

Actin

  • Globular (G-actin) and fibrous (F-actin) forms.
  • G-actin molecular weight is around 42,000-48,000 Daltons.
  • F-actin is a polymer of G-actin with a 92% alpha-helix structure.

Tropomyosin

  • 2 stranded alpha helix protein, representing ~5% of muscle protein. Typical molecular weight is 33,000 - 37,000 Daltons.
  • Length is around 400 Ã…. Lies within actin's double helix groove. Each tropomyosin interacts with 7 G-actin units.

Connective Tissue

  • Elastin and Collagen, important protein components of connective tissue.
  • Collagen (principal connective tissue protein) is composed of tropocollagen units.
  • Toughness of muscle tissue is related to collagen content. Collagen is converted to gelatin by moist heat treatment.

Adipose Tissue

  • Adipocytes (fat cells)
  • Surface fat (subcutaneous)
  • Intramuscular fat (marbling)
  • Marbling is related to tenderness and flavour grade of meat.

Post-mortem Changes in Muscle

  • Biochemical reactions following animal death lead to muscle conversion into meat.
  • Aerobic pathway ceases upon blood removal at slaughter.
  • Anaerobic pathway begins temporarily, converting glucose to lactic acid, impacting pH and meat quality.
  • A drop in pH affects meat quality.

Effect of pH on Meat Quality

  • Rapid pH decrease can trigger protein denaturation, leading to lower water-holding capacity and pale myoglobin.
  • High pH might result in darker meat color and dry meat surface.

pH Decline in Meats

  • Graph displays pH change over 24 hours post-mortem for different carcass types (DFD, Normal, PSE).

Generation of Meat Flavor

  • ATP degradation during aging produces ADP, AMP, IMP (inosinemonophosphate), and ammonia, contributing to cooked meat flavor.

Rigor Mortis and Aging

  • Rigor mortis is the post-mortem stiffening of the carcass. Depletion of muscle ATP leads to actomyosin protein formation.
  • Aging is the process of allowing rigor's resolution (muscle relaxation). Structural alterations in the myofibrils happen during this process.
  • Aging is associated with loss of muscle elasticity, increase in muscle tension, and sarcomere shortening, which can affect meat toughness.

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