16 Questions
Which method can be used to isolate and obtain a pure culture of plant pathogenic fungus?
Spread plate method
What precautionary measure is recommended to prevent contamination during isolation of bacteria?
Flaming the needle before laying it down
How is a pure culture defined?
Containing only one kind of microorganism
Which activity is prohibited during the isolation of bacteria to prevent contamination?
Talking
What is the purpose of using the streak plate, pour plate, and spread plate methods for isolating bacteria?
To obtain pure bacterium culture from a mixture
What is the purpose of the Streak Plate Method?
Isolating pure cultures of bacteria
What is the purpose of serial dilution in microbiology?
To reduce a dense culture of cells to a more usable concentration
What is the purpose of incubating plates in an inverted position?
To prevent condensation from dripping onto the culture
In the Tissue Plating Method for fungi, what is done after mycelial growths emerge from the edges of plated tissues?
Further tests are conducted on selected plates
Why is it important to seal all plates with cling wrap and masking tape before incubation?
To prevent external contamination
What does CFU/mL stand for in microbiology?
Colony Forming Unit per milliliter
How is 'viable' defined in the context of microbiology?
Ability to multiply via binary fission under controlled conditions
What does a dilution factor of 10-1 indicate?
The sample was diluted by a factor of 100
In a pour plate with 10-2 dilution, if there are 20 colonies observed, what is the CFU/mL?
2000 CFU/mL
What does 'Nonquantifiable' mean when referring to results from a streak plate?
No growth observed
Why is the concept of CFU/mL important in microbiology lab procedures?
To ensure accurate dilutions are made for counting viable cells
Explore methods for isolating and obtaining pure cultures of bacteria and fungi, such as streak plate, pour plate, and spread plate techniques. Learn to isolate plant pathogenic fungus, Helminthosporium maydis, from a corn leaf. Adapted from Micr 23n BSFT Students' terminal report at Visayas State University.
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