Isolation and Enumeration of Bacteria and Fungi
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Questions and Answers

Which method can be used to isolate and obtain a pure culture of plant pathogenic fungus?

  • Quantifiable method
  • Spread plate method (correct)
  • Non-quantifiable method
  • Serial dilution method

    • What precautionary measure is recommended to prevent contamination during isolation of bacteria?

  • Opening the culture tubes in close proximity
  • Talking in a low voice
  • Flaming the needle before laying it down (correct)
  • Leaving the fans on for circulation

    • How is a pure culture defined?

  • Containing multiple kinds of microorganisms
  • Containing only one kind of microorganism (correct)
  • Having a mix of bacteria and fungi
  • Being isolated from a single source

    • Which activity is prohibited during the isolation of bacteria to prevent contamination?

    <p>Talking</p> Signup and view all the answers

    What is the purpose of using the streak plate, pour plate, and spread plate methods for isolating bacteria?

    <p>To obtain pure bacterium culture from a mixture</p> Signup and view all the answers

    What is the purpose of the Streak Plate Method?

    <p>Isolating pure cultures of bacteria</p> Signup and view all the answers

    What is the purpose of serial dilution in microbiology?

    <p>To reduce a dense culture of cells to a more usable concentration</p> Signup and view all the answers

    What is the purpose of incubating plates in an inverted position?

    <p>To prevent condensation from dripping onto the culture</p> Signup and view all the answers

    In the Tissue Plating Method for fungi, what is done after mycelial growths emerge from the edges of plated tissues?

    <p>Further tests are conducted on selected plates</p> Signup and view all the answers

    Why is it important to seal all plates with cling wrap and masking tape before incubation?

    <p>To prevent external contamination</p> Signup and view all the answers

    What does CFU/mL stand for in microbiology?

    <p>Colony Forming Unit per milliliter</p> Signup and view all the answers

    How is 'viable' defined in the context of microbiology?

    <p>Ability to multiply via binary fission under controlled conditions</p> Signup and view all the answers

    What does a dilution factor of 10-1 indicate?

    <p>The sample was diluted by a factor of 100</p> Signup and view all the answers

    In a pour plate with 10-2 dilution, if there are 20 colonies observed, what is the CFU/mL?

    <p>2000 CFU/mL</p> Signup and view all the answers

    What does 'Nonquantifiable' mean when referring to results from a streak plate?

    <p>No growth observed</p> Signup and view all the answers

    Why is the concept of CFU/mL important in microbiology lab procedures?

    <p>To ensure accurate dilutions are made for counting viable cells</p> Signup and view all the answers

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