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Match the following types of microscopes with their descriptions:
Match the following types of microscopes with their descriptions:
Light microscope (LM) = Uses white or ultraviolet light, with a resolving power of 0.2 μm and maximal theoretical magnification of 2,000 times Electron microscope (EM) = Uses a stream of electrons emitted by cathode
Match the following parts of a light microscope with their functions:
Match the following parts of a light microscope with their functions:
Optical parts made from cut glass = Used for transmission of light and magnification of the image Slides (native or fixed) = Usually observed in passing light under routine conditions
Match the following sources of light with their usage in microscopes:
Match the following sources of light with their usage in microscopes:
Sunlight, bulb or vapour lamp = Used as a source of light in light microscopes Method of lighting from above slides = Mainly used in fluorescence and inverted microscopes
Match the following disciplines with their use of microscopic techniques:
Match the following disciplines with their use of microscopic techniques:
Match the following types of microscopes with their respective radiation:
Match the following types of microscopes with their respective radiation:
Match the following features with their descriptions for light microscopes:
Match the following features with their descriptions for light microscopes:
Match the following terms with their usage in light microscopes:
Match the following terms with their usage in light microscopes:
Match the following types of microscopes with their respective disciplines using them:
Match the following types of microscopes with their respective disciplines using them:
Match the following terms with their usage in electron microscopes:
Match the following terms with their usage in electron microscopes:
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Study Notes
Types of Microscopes
- Light Microscopes: Utilize visible light and lenses to magnify specimens.
- Electron Microscopes: Employ electron beams for higher resolution images, effective for cellular and molecular structures.
- Fluorescence Microscopes: Use fluorescent dyes and lamps to visualize structures within cells.
- Confocal Microscopes: Create sharp images of thick specimens by focusing on specific depths with laser light.
Parts of a Light Microscope and Their Functions
- Eyepiece: Contains a lens to magnify the image observed.
- Objective Lens: Various lenses that provide different magnifications.
- Stage: Platform where specimens are placed for observation.
- Diaphragm: Controls the amount of light reaching the specimen.
Sources of Light in Microscopes
- LED Light: Commonly used in modern microscopes for bright, energy-efficient illumination.
- Halogen Bulbs: Provide intense white light, used in higher-end lab microscopes.
- Mercury Vapor Lamps: Emit high-intensity UV light for fluorescence microscopy.
- Xenon Light: Produces broad-spectrum illumination, often in advanced microscopy.
Disciplines and Microscopic Techniques
- Cell Biology: Utilizes light and electron microscopy to examine cellular structures.
- Microbiology: Employs various microscopes to visualize bacteria and microorganisms.
- Pathology: Uses microscopy for analyzing tissues and diagnosing diseases.
- Materials Science: Applies electron microscopy to study material properties at microscopic levels.
Types of Microscopes and Their Radiation
- Light Microscope: Uses visible light for illumination.
- Scanning Electron Microscope (SEM): Utilizes electron beams to generate 3D images of surfaces.
- Transmission Electron Microscope (TEM): Employs electron transmission through thin specimens for high-resolution images.
Features of Light Microscopes
- Limited Resolution: Typically up to 200 nm due to the wavelength of light.
- Color Visualization: Can show colors of stained specimens.
- Live Cell Imaging: Allows observation of live cells under controlled conditions.
Terms and Usage in Light Microscopes
- Resolution: The ability to distinguish two closely spaced points.
- Magnification: The increase in apparent size of an object.
- Staining: Enhances contrast in specimens to visualize structures.
Types of Microscopes and Their Respective Disciplines
- Phase Contrast Microscopes: Common in cellular biology for viewing live cells.
- Dark Field Microscopes: Used in microbiology to see unstained specimens like bacteria.
- Fluorescence Microscopes: Frequently employed in genetics and molecular biology.
Terms and Usage in Electron Microscopes
- Sample Preparation: Involves fixing and embedding samples to withstand electron beam conditions.
- Vacuum Environment: Required to prevent electron scattering by air molecules.
- Magnification Power: Can exceed one million times, allowing for detailed exploration of cellular structures.
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