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Questions and Answers
Match the following types of microscopes with their descriptions:
Match the following types of microscopes with their descriptions:
Light microscope (LM) = Uses white or ultraviolet light, with a resolving power of 0.2 μm and maximal theoretical magnification of 2,000 times Electron microscope (EM) = Uses a stream of electrons emitted by cathode
Match the following parts of a light microscope with their functions:
Match the following parts of a light microscope with their functions:
Optical parts made from cut glass = Used for transmission of light and magnification of the image Slides (native or fixed) = Usually observed in passing light under routine conditions
Match the following sources of light with their usage in microscopes:
Match the following sources of light with their usage in microscopes:
Sunlight, bulb or vapour lamp = Used as a source of light in light microscopes Method of lighting from above slides = Mainly used in fluorescence and inverted microscopes
Match the following disciplines with their use of microscopic techniques:
Match the following disciplines with their use of microscopic techniques:
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Match the following types of microscopes with their respective radiation:
Match the following types of microscopes with their respective radiation:
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Match the following features with their descriptions for light microscopes:
Match the following features with their descriptions for light microscopes:
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Match the following terms with their usage in light microscopes:
Match the following terms with their usage in light microscopes:
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Match the following types of microscopes with their respective disciplines using them:
Match the following types of microscopes with their respective disciplines using them:
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Match the following terms with their usage in electron microscopes:
Match the following terms with their usage in electron microscopes:
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Study Notes
Types of Microscopes
- Light Microscopes: Utilize visible light and lenses to magnify specimens.
- Electron Microscopes: Employ electron beams for higher resolution images, effective for cellular and molecular structures.
- Fluorescence Microscopes: Use fluorescent dyes and lamps to visualize structures within cells.
- Confocal Microscopes: Create sharp images of thick specimens by focusing on specific depths with laser light.
Parts of a Light Microscope and Their Functions
- Eyepiece: Contains a lens to magnify the image observed.
- Objective Lens: Various lenses that provide different magnifications.
- Stage: Platform where specimens are placed for observation.
- Diaphragm: Controls the amount of light reaching the specimen.
Sources of Light in Microscopes
- LED Light: Commonly used in modern microscopes for bright, energy-efficient illumination.
- Halogen Bulbs: Provide intense white light, used in higher-end lab microscopes.
- Mercury Vapor Lamps: Emit high-intensity UV light for fluorescence microscopy.
- Xenon Light: Produces broad-spectrum illumination, often in advanced microscopy.
Disciplines and Microscopic Techniques
- Cell Biology: Utilizes light and electron microscopy to examine cellular structures.
- Microbiology: Employs various microscopes to visualize bacteria and microorganisms.
- Pathology: Uses microscopy for analyzing tissues and diagnosing diseases.
- Materials Science: Applies electron microscopy to study material properties at microscopic levels.
Types of Microscopes and Their Radiation
- Light Microscope: Uses visible light for illumination.
- Scanning Electron Microscope (SEM): Utilizes electron beams to generate 3D images of surfaces.
- Transmission Electron Microscope (TEM): Employs electron transmission through thin specimens for high-resolution images.
Features of Light Microscopes
- Limited Resolution: Typically up to 200 nm due to the wavelength of light.
- Color Visualization: Can show colors of stained specimens.
- Live Cell Imaging: Allows observation of live cells under controlled conditions.
Terms and Usage in Light Microscopes
- Resolution: The ability to distinguish two closely spaced points.
- Magnification: The increase in apparent size of an object.
- Staining: Enhances contrast in specimens to visualize structures.
Types of Microscopes and Their Respective Disciplines
- Phase Contrast Microscopes: Common in cellular biology for viewing live cells.
- Dark Field Microscopes: Used in microbiology to see unstained specimens like bacteria.
- Fluorescence Microscopes: Frequently employed in genetics and molecular biology.
Terms and Usage in Electron Microscopes
- Sample Preparation: Involves fixing and embedding samples to withstand electron beam conditions.
- Vacuum Environment: Required to prevent electron scattering by air molecules.
- Magnification Power: Can exceed one million times, allowing for detailed exploration of cellular structures.
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Description
Learn about the types and design of microscopes, as well as the principles of their work and their use in biomedical disciplines. This quiz provides basic information essential for understanding microscopic techniques in the field of cytology and genetics.
