Introduction to Mass Spectrometry

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What happens when molecules in gas state are bombarded with high energy electrons?

They lose an electron and form a molecular ion (M+)

What does mass spectrometry provide information about?

Exact molecular weight and molecular formula

What does the intensity of each signal in mass spectrometry represent?

Relative abundance of the cations

What is a molecular ion (M+)?

A radical cation with a positive charge and one unpaired electron, representing the mass of the original molecule

What is separated by magnetic deflection in mass spectrometry?

Cations based on their (m/z) ratio

Study Notes

Mass Spectrometry

  • When molecules in a gas state are bombarded with high-energy electrons, they break into charged particles called ions.
  • Mass spectrometry provides information about the mass-to-charge ratio of ions, which helps identify the structure of molecules.

Signal Intensity

  • The intensity of each signal in mass spectrometry represents the abundance of ions with a specific mass-to-charge ratio.

Molecular Ion

  • A molecular ion (M+) is a molecule that has lost or gained one or more electrons to form an ion with a single charge.

Ion Separation

  • In mass spectrometry, ions are separated by magnetic deflection according to their mass-to-charge ratio.

This quiz covers the basic principles of mass spectrometry, focusing on the detection of molecular weight and the process of ionization and fragmentation of molecules. It is designed to test understanding of how mass spectrometry provides information about molecular formula and structural elucidation of drugs.

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