12 Questions
What is the primary aim of tissue fixation in histology preparation?
To preserve the morphology of cells
Which step comes immediately after clearing in the basic steps of tissue preparation for histology?
Infiltration or impregnation
What is the purpose of staining in histological examination?
To enhance visualization of cellular components
Why is fixation necessary in tissue preparation for histology?
To preserve the structure and molecular composition of cells
What is the recommended pH range for fixation?
6-8
At what temperature is fixation generally carried out for light microscopy?
45°C
Which factor affects the speed of decalcification the most?
Type of decalcifying agent
What is the purpose of dehydrating tissues after fixation?
To prepare tissues for staining
What is the main purpose of embedding a specimen in wax before section cutting?
To make the tissue block easier to handle in the microtome
What is the purpose of the trimming process in histological techniques?
To remove excess wax from the tissue block
Which type of dye is used to stain nucleic acids and glycosaminoglycans in histological staining?
Basophilic dyes
What is the typical thickness range for paraffin sections in histological techniques?
4-6 microns
Learn about histology, which involves the study of tissues and how they form organs in the body. Explore the structure and arrangement of cells to optimize organ functions.
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