Podcast
Questions and Answers
What is the primary aim of tissue fixation in histology preparation?
What is the primary aim of tissue fixation in histology preparation?
- To enhance the stain absorption
- To preserve the morphology of cells (correct)
- To optimize the function of the cells
- To harden the tissue to protect from trauma
Which step comes immediately after clearing in the basic steps of tissue preparation for histology?
Which step comes immediately after clearing in the basic steps of tissue preparation for histology?
- Sectioning or cutting
- Infiltration or impregnation (correct)
- Labeling
- Mounting
What is the purpose of staining in histological examination?
What is the purpose of staining in histological examination?
- To harden the tissues for protection
- To enhance visualization of cellular components (correct)
- To remove excess moisture from tissues
- To increase tissue transparency
Why is fixation necessary in tissue preparation for histology?
Why is fixation necessary in tissue preparation for histology?
What is the recommended pH range for fixation?
What is the recommended pH range for fixation?
At what temperature is fixation generally carried out for light microscopy?
At what temperature is fixation generally carried out for light microscopy?
Which factor affects the speed of decalcification the most?
Which factor affects the speed of decalcification the most?
What is the purpose of dehydrating tissues after fixation?
What is the purpose of dehydrating tissues after fixation?
What is the main purpose of embedding a specimen in wax before section cutting?
What is the main purpose of embedding a specimen in wax before section cutting?
What is the purpose of the trimming process in histological techniques?
What is the purpose of the trimming process in histological techniques?
Which type of dye is used to stain nucleic acids and glycosaminoglycans in histological staining?
Which type of dye is used to stain nucleic acids and glycosaminoglycans in histological staining?
What is the typical thickness range for paraffin sections in histological techniques?
What is the typical thickness range for paraffin sections in histological techniques?
