Introduction to Histology: The Study of Body Tissues

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What is the primary aim of tissue fixation in histology preparation?

To enhance the stain absorption

To preserve the morphology of cells (correct)

To optimize the function of the cells

To harden the tissue to protect from trauma

Which step comes immediately after clearing in the basic steps of tissue preparation for histology?

Sectioning or cutting

Infiltration or impregnation (correct)

Labeling

Mounting

What is the purpose of staining in histological examination?

To harden the tissues for protection

To enhance visualization of cellular components (correct)

To remove excess moisture from tissues

To increase tissue transparency

Why is fixation necessary in tissue preparation for histology?

To preserve the structure and molecular composition of cells Signup and view all the answers

What is the recommended pH range for fixation?

6-8 Signup and view all the answers

At what temperature is fixation generally carried out for light microscopy?

45°C Signup and view all the answers

Which factor affects the speed of decalcification the most?

Type of decalcifying agent Signup and view all the answers

What is the purpose of dehydrating tissues after fixation?

To prepare tissues for staining Signup and view all the answers

What is the main purpose of embedding a specimen in wax before section cutting?

To make the tissue block easier to handle in the microtome Signup and view all the answers

What is the purpose of the trimming process in histological techniques?

To remove excess wax from the tissue block Signup and view all the answers

Which type of dye is used to stain nucleic acids and glycosaminoglycans in histological staining?

Basophilic dyes Signup and view all the answers

What is the typical thickness range for paraffin sections in histological techniques?

4-6 microns Signup and view all the answers

Introduction to Histology: The Study of Body Tissues

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Questions and Answers

What is the primary aim of tissue fixation in histology preparation?

Which step comes immediately after clearing in the basic steps of tissue preparation for histology?

What is the purpose of staining in histological examination?

Why is fixation necessary in tissue preparation for histology?

What is the recommended pH range for fixation?

At what temperature is fixation generally carried out for light microscopy?

Which factor affects the speed of decalcification the most?

What is the purpose of dehydrating tissues after fixation?

What is the main purpose of embedding a specimen in wax before section cutting?

What is the purpose of the trimming process in histological techniques?

Which type of dye is used to stain nucleic acids and glycosaminoglycans in histological staining?

What is the typical thickness range for paraffin sections in histological techniques?

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