Introduction to Histology: The Study of Body Tissues
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Questions and Answers

What is the primary aim of tissue fixation in histology preparation?

  • To enhance the stain absorption
  • To preserve the morphology of cells (correct)
  • To optimize the function of the cells
  • To harden the tissue to protect from trauma
  • Which step comes immediately after clearing in the basic steps of tissue preparation for histology?

  • Sectioning or cutting
  • Infiltration or impregnation (correct)
  • Labeling
  • Mounting
  • What is the purpose of staining in histological examination?

  • To harden the tissues for protection
  • To enhance visualization of cellular components (correct)
  • To remove excess moisture from tissues
  • To increase tissue transparency
  • Why is fixation necessary in tissue preparation for histology?

    <p>To preserve the structure and molecular composition of cells</p> Signup and view all the answers

    What is the recommended pH range for fixation?

    <p>6-8</p> Signup and view all the answers

    At what temperature is fixation generally carried out for light microscopy?

    <p>45°C</p> Signup and view all the answers

    Which factor affects the speed of decalcification the most?

    <p>Type of decalcifying agent</p> Signup and view all the answers

    What is the purpose of dehydrating tissues after fixation?

    <p>To prepare tissues for staining</p> Signup and view all the answers

    What is the main purpose of embedding a specimen in wax before section cutting?

    <p>To make the tissue block easier to handle in the microtome</p> Signup and view all the answers

    What is the purpose of the trimming process in histological techniques?

    <p>To remove excess wax from the tissue block</p> Signup and view all the answers

    Which type of dye is used to stain nucleic acids and glycosaminoglycans in histological staining?

    <p>Basophilic dyes</p> Signup and view all the answers

    What is the typical thickness range for paraffin sections in histological techniques?

    <p>4-6 microns</p> Signup and view all the answers

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