Introduction to Chromatography Quiz

Chromatography Overview

• Chromatography is a technique used to separate mixtures into their individual components.
• It is widely utilized in laboratories for analyzing substances in chemistry, biology, and environmental science.

Inventor of Liquid Chromatography

• Martin and Synge are credited with inventing and naming liquid chromatography in the early 20th century.

Meaning of Chromatography

• The term 'chromatography' derives from the Greek words "chroma" (color) and "grapho" (to write), originally pertaining to color separation observed in the technique.

Substances Separated by Chromatography

• Various substances can be separated, including gases, liquids, and dissolved solids, such as organic compounds, amino acids, and carbohydrates.

Purpose of Chromatography

• The main purpose is to purify compounds, identify substances, and analyze mixtures for quality control and research.

Mobile Phase in Gas Chromatography

• The mobile phase in gas chromatography consists of an inert gas, commonly helium or nitrogen, that transports the sample through the system.

Effective Gas Chromatography Type

• The most useful type for separating gases such as methane (CH4) and carbon dioxide (CO2) is capillary gas chromatography.

Stationary Phase in Gas Liquid Chromatography (GLC)

• In GLC, the stationary phase is a liquid coated onto a solid support material that helps retain the sample components as they move through.

Affinity in Gas Solid Chromatography (GSC)

• In GSC, the affinity of solutes is towards the solid stationary phase, allowing for separation based on adsorption characteristics.

Mechanism of Separation in Gas Chromatography

• Separation of components in gas chromatography occurs due to differences in volatility and interaction with the stationary phase, leading to varied retention times.