Introduction to Bacterial Gene Regulation Lecture
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Questions and Answers

Explain the role of riboswitches in gene expression control.

Riboswitches are sensory RNA molecules that fold in response to metabolites or other effectors, influencing mRNA transcription continuation, half-life, and translation.

How do sigma factors contribute to transcriptional control?

Sigma factors are involved in recognizing promoters and enhancing specificity in transcription, thereby regulating gene expression.

Discuss the post-transcriptional control of translation in gene expression.

Post-transcriptional control involves mechanisms like riboswitches, regulation of mRNA stability, and occlusion of the Shine Dalgarno site to modulate translation.

How do tRNAs influence gene expression in bacteria?

<p>tRNAs, like in the T box system, can impact the expression of specific genes, such as tyrosyl tRNA synthetase in Gram-positive bacteria.</p> Signup and view all the answers

Explain the significance of hierarchical mechanisms controlling gene expression.

<p>Hierarchical control mechanisms help coordinate different levels of gene expression regulation, ensuring precise responses to environmental cues.</p> Signup and view all the answers

How do riboswitches respond to environmental cues?

<p>Riboswitches fold in response to specific metabolites or effectors, altering their structure to modulate gene expression.</p> Signup and view all the answers

What is the function of the Shine Dalgarno sequence in bacteria?

<p>The Shine Dalgarno sequence serves as a ribosomal binding site, facilitating the initiation of translation of mRNA into protein.</p> Signup and view all the answers

How do bacteria regulate gene expression in response to DNA damage?

<p>Bacteria employ the SOS response as a global response to sudden global DNA damage, which involves the expression of a subset of proteins to permit the bacterium to survive current conditions.</p> Signup and view all the answers

What role do sigma factors play in the regulation of gene expression in bacteria?

<p>Sigma factors bind to RNA polymerase and help determine the promoter recognition specificity of the enzyme, leading to the transcription of specific genes.</p> Signup and view all the answers

What is the purpose of the lac operon in bacteria, and how is it regulated?

<p>The lac operon allows bacteria to utilize lactose as an energy source. The operon is regulated by the lac repressor protein, which binds to the operator site and prevents the transcription of the lac genes in the absence of lactose.</p> Signup and view all the answers

What is the significance of the 5' untranslated region (UTR) and the 3' untranslated region (UTR) in gene expression?

<p>The 5' UTR contains important regulatory elements such as the Shine Dalgarno sequence, while the 3' UTR plays a role in mRNA stability and localization.</p> Signup and view all the answers

What are some mechanisms for controlling protein function in bacteria?

<p>Mechanisms for controlling protein function in bacteria include post-translational modifications, allostery, and protein-protein interactions.</p> Signup and view all the answers

What is the role of the thermosensitive riboswitch in regulating RpoH expression during heat stress?

<p>It acts as a thermostat and signal for degradation, preventing translation at normal temperatures and allowing translation during heat shock.</p> Signup and view all the answers

How does the DnaK/DnaJ/GrpE chaperone system regulate RpoH levels during heat shock?

<p>RpoH is released from the chaperone, allowing it to associate with RNA polymerase and increase transcription from RpoH-dependent promoters.</p> Signup and view all the answers

What is the significance of the feedback loop involving RpoH, DnaK, and GrpE?

<p>RpoH levels decline in the cytoplasm, reducing the amount of sigma factor binding RNA polymerase, and thus decreasing transcription from RpoH-dependent promoters.</p> Signup and view all the answers

Describe the three different types of regulatory control in bacterial gene regulation.

<p>The three different types of regulatory control are transcriptional, post-transcriptional, and post-translational. Transcriptional control involves regulation at the level of transcription, post-transcriptional control involves regulation at the level of RNA processing and stability, and post-translational control involves regulation at the level of protein activity and stability.</p> Signup and view all the answers

What is the role of ClpB in the heat shock response?

<p>ClpB is a foldase that re-folds proteins to their correct conformation, helping to restore normal function.</p> Signup and view all the answers

How does the RpoH-dependent transcription system respond to heat stress in the cytoplasm?

<p>RpoH associates with RNA polymerase, increasing transcription from RpoH-dependent promoters, leading to the expression of heat shock genes.</p> Signup and view all the answers

Explain what sigma factors are and their role in transcription.

<p>Sigma factors are proteins that bind to the RNA polymerase core enzyme and direct it to recognize specific promoter sequences on DNA. They play a crucial role in transcription initiation by helping to recruit RNA polymerase to the promoter and unwind the DNA double helix.</p> Signup and view all the answers

Describe the mechanism of action of riboswitches and which type of regulation they contribute to.

<p>Riboswitches are RNA molecules that can bind specific metabolites or other small molecules to change their conformation, thereby regulating gene expression. They contribute to post-transcriptional regulation by controlling mRNA processing, stability, or translation.</p> Signup and view all the answers

What is the limitation of the thermosensory mRNA mechanism in responding to heat stress?

<p>It only allows for rapid responses to short exposures to heat shock, but is not effective for prolonged heat stress or in the periplasm.</p> Signup and view all the answers

Explain the post-translational regulation of RpoH and RpoE and the controlling feedback loop.

<p>RpoH and RpoE are both heat shock transcription factors that are tightly regulated at the post-translational level. RpoH is degraded by the protease FtsH, which is inhibited at high temperatures, allowing for increased RpoH activity. RpoE is regulated by a feedback loop involving its own gene and the protease DegS, which senses misfolded outer membrane proteins and activates RpoE in response.</p> Signup and view all the answers

What is the difference between a regulon and an operon in bacterial gene regulation?

<p>An operon is a group of genes that are coordinately regulated by a single promoter, while a regulon is a group of genes that are regulated by a common set of factors, which may be located in different regions of the genome.</p> Signup and view all the answers

Why is the central dogma of molecular biology important for understanding bacterial gene regulation?

<p>The central dogma of molecular biology outlines the flow of genetic information from DNA to RNA to protein, which is the basis for understanding the different levels of gene regulation in bacteria. By understanding the central dogma, we can better understand how gene expression is controlled at each stage of the process.</p> Signup and view all the answers

Describe the conditions under which Sigma E is sequestered to the inner membrane.

<p>Normal growth conditions, where Sigma E is sequestered by RseA and RseB, and RpoE associates with RNA polymerase only at low levels.</p> Signup and view all the answers

What is the role of DegS protease in the transmission of environmental signals across bacterial compartments?

<p>DegS unfolds and becomes active during stress, degrading misfolded proteins, and releasing RpoE from RseA and RseB.</p> Signup and view all the answers

How does the stress response from RpoE dependent promoters, including RpoH, get activated?

<p>RpoE associates with RNA polymerase, inducing the stress response, which includes the expression of RpoH.</p> Signup and view all the answers

What happens once all misfolded proteins are removed in the feedback loop of the RpoE system?

<p>RpoE re-associates with RseAB, shutting off the response.</p> Signup and view all the answers

What is the role of YaeL protease in the transmission of environmental signals across bacterial compartments?

<p>YaeL protease releases RpoE from RseA and RseB.</p> Signup and view all the answers

What is the ultimate outcome of the stress response triggered by RpoE, including the expression of RpoH, if the response is to heat?

<p>The increased mRNA from RpoH will unwind and be translated.</p> Signup and view all the answers

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