Interpretation of Enzyme Kinetic Equations and Constants

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When [S] is low, what is the relationship between V0 and Vmax?

V0 = Vmax/2

V0 is independent of Vmax

V0 = Vmax (correct)

V0 = 2Vmax

What is the significance of Km in enzyme kinetics?

It represents the maximum velocity of the reaction

It is the equilibrium constant of the association of E and S

It indicates the substrate concentration needed to reach half the maximum velocity (correct)

It is the rate constant of the reaction

What is the relationship between [ES] and [Etotal] when [S] = Km?

[ES] = [Etotal]

[ES] = 2[Etotal]

[ES] = [Etotal]/2 (correct)

[ES] is independent of [Etotal]

What is the rapid equilibrium assumption in enzyme kinetics?

The dissociation of ES into E and S is faster than the association of E and S Signup and view all the answers

What does a high value of Km indicate?

Weak association of E and S Signup and view all the answers

What is one of the uses of Km in enzyme kinetics?

To establish an approximate value for the intracellular level of the substrate Signup and view all the answers

What is the purpose of comparing Km values from different enzymes?

To compare the suitability of alternate substrates for an enzyme Signup and view all the answers

What does a decrease in Km value indicate?

An increase in the enzyme's affinity for the substrate Signup and view all the answers

What is the unit of the turn over number (K2) of an enzyme?

Molecules per unit time Signup and view all the answers

What is the significance of the Vmax/Km ratio?

It determines the best substrate for the enzyme Signup and view all the answers

What is the effect of a ligand on the Km value of an enzyme?

It changes the Km value, indicating a regulatory mechanism Signup and view all the answers

What is the maximum velocity (Vmax) of an enzyme?

The highest rate of substrate conversion to product Signup and view all the answers

Interpretation of Enzyme Kinetic Equations and Constants

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Questions and Answers

When [S] is low, what is the relationship between V0 and Vmax?

What is the significance of Km in enzyme kinetics?

What is the relationship between [ES] and [Etotal] when [S] = Km?

What is the rapid equilibrium assumption in enzyme kinetics?

What does a high value of Km indicate?

What is one of the uses of Km in enzyme kinetics?

What is the purpose of comparing Km values from different enzymes?

What does a decrease in Km value indicate?

What is the unit of the turn over number (K2) of an enzyme?

What is the significance of the Vmax/Km ratio?

What is the effect of a ligand on the Km value of an enzyme?

What is the maximum velocity (Vmax) of an enzyme?

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