Interpretation of Enzyme Kinetic Equations and Constants
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Questions and Answers

When [S] is low, what is the relationship between V0 and Vmax?

  • V0 = Vmax/2
  • V0 is independent of Vmax
  • V0 = Vmax (correct)
  • V0 = 2Vmax
  • What is the significance of Km in enzyme kinetics?

  • It represents the maximum velocity of the reaction
  • It is the equilibrium constant of the association of E and S
  • It indicates the substrate concentration needed to reach half the maximum velocity (correct)
  • It is the rate constant of the reaction
  • What is the relationship between [ES] and [Etotal] when [S] = Km?

  • [ES] = [Etotal]
  • [ES] = 2[Etotal]
  • [ES] = [Etotal]/2 (correct)
  • [ES] is independent of [Etotal]
  • What is the rapid equilibrium assumption in enzyme kinetics?

    <p>The dissociation of ES into E and S is faster than the association of E and S</p> Signup and view all the answers

    What does a high value of Km indicate?

    <p>Weak association of E and S</p> Signup and view all the answers

    What is one of the uses of Km in enzyme kinetics?

    <p>To establish an approximate value for the intracellular level of the substrate</p> Signup and view all the answers

    What is the purpose of comparing Km values from different enzymes?

    <p>To compare the suitability of alternate substrates for an enzyme</p> Signup and view all the answers

    What does a decrease in Km value indicate?

    <p>An increase in the enzyme's affinity for the substrate</p> Signup and view all the answers

    What is the unit of the turn over number (K2) of an enzyme?

    <p>Molecules per unit time</p> Signup and view all the answers

    What is the significance of the Vmax/Km ratio?

    <p>It determines the best substrate for the enzyme</p> Signup and view all the answers

    What is the effect of a ligand on the Km value of an enzyme?

    <p>It changes the Km value, indicating a regulatory mechanism</p> Signup and view all the answers

    What is the maximum velocity (Vmax) of an enzyme?

    <p>The highest rate of substrate conversion to product</p> Signup and view all the answers

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