Interpretation of Enzyme Kinetic Equations and Constants
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Questions and Answers

When [S] is low, what is the relationship between V0 and Vmax?

  • V0 = Vmax/2
  • V0 is independent of Vmax
  • V0 = Vmax (correct)
  • V0 = 2Vmax

What is the significance of Km in enzyme kinetics?

  • It represents the maximum velocity of the reaction
  • It is the equilibrium constant of the association of E and S
  • It indicates the substrate concentration needed to reach half the maximum velocity (correct)
  • It is the rate constant of the reaction

What is the relationship between [ES] and [Etotal] when [S] = Km?

  • [ES] = [Etotal]
  • [ES] = 2[Etotal]
  • [ES] = [Etotal]/2 (correct)
  • [ES] is independent of [Etotal]

What is the rapid equilibrium assumption in enzyme kinetics?

<p>The dissociation of ES into E and S is faster than the association of E and S (C)</p> Signup and view all the answers

What does a high value of Km indicate?

<p>Weak association of E and S (D)</p> Signup and view all the answers

What is one of the uses of Km in enzyme kinetics?

<p>To establish an approximate value for the intracellular level of the substrate (A)</p> Signup and view all the answers

What is the purpose of comparing Km values from different enzymes?

<p>To compare the suitability of alternate substrates for an enzyme (C)</p> Signup and view all the answers

What does a decrease in Km value indicate?

<p>An increase in the enzyme's affinity for the substrate (B)</p> Signup and view all the answers

What is the unit of the turn over number (K2) of an enzyme?

<p>Molecules per unit time (A)</p> Signup and view all the answers

What is the significance of the Vmax/Km ratio?

<p>It determines the best substrate for the enzyme (B)</p> Signup and view all the answers

What is the effect of a ligand on the Km value of an enzyme?

<p>It changes the Km value, indicating a regulatory mechanism (A)</p> Signup and view all the answers

What is the maximum velocity (Vmax) of an enzyme?

<p>The highest rate of substrate conversion to product (D)</p> Signup and view all the answers

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