Interactions of Light with Objects

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Questions and Answers

What is resolution in microscopy?

  • The smallest distance by which two objects can be separated and still be distinguished (correct)
  • The ability to determine the presence of an object
  • An increase in the apparent size of an image
  • The area difference between two objects

Which type of microscopy is the simplest?

  • Scanning probe microscopy
  • Electron microscopy
  • Fluorescence microscopy
  • Light microscopy (correct)

What is detection in terms of observing microbes?

  • Resolution of an image
  • Increase in magnification
  • Differentiation of microbial shapes
  • Determining the presence of an object (correct)

What is magnification in microscopy?

<p>An increase in the apparent size of an image to resolve smaller separations between objects (D)</p> Signup and view all the answers

What range of sizes do eukaryotic microbes like protozoa, algae, and fungi typically fall within?

<p>2 µm–20 cm (A)</p> Signup and view all the answers

What is the main purpose of a simple stain in microbiology?

<p>Add dark color specifically to cells (A)</p> Signup and view all the answers

Which technique is used to differentiate between Gram-positive and Gram-negative bacteria?

<p>Gram Stain (C)</p> Signup and view all the answers

What happens during fluorescence microscopy in terms of light absorption and emission?

<p>Specimen absorbs light of defined wavelength and emits light of lower energy (A)</p> Signup and view all the answers

Which staining technique is known for staining different types of cells to distinguish them from each other?

<p>Differential Stain (A)</p> Signup and view all the answers

What is the function of a counterstain in the Gram stain technique?

<p>To stain Gram-negative bacteria red or pink (B)</p> Signup and view all the answers

What is the typical size range of prokaryotes?

<p>0.4–10 µm (B)</p> Signup and view all the answers

What are common shapes seen in bacteria?

<p>Bacilli and cocci (B)</p> Signup and view all the answers

What are the key conditions needed for electromagnetic radiation to resolve an object?

<p>Contrast between object and medium, smaller wavelength than the object (D)</p> Signup and view all the answers

What is the wavelength range of visible light?

<p>~400–750 nm (C)</p> Signup and view all the answers

How small must the wavelength be compared to the object for electromagnetic radiation to resolve it?

<p>Smaller than the object (D)</p> Signup and view all the answers

What does absorption of light by an object mean?

<p>The object acquires the photon's energy (A)</p> Signup and view all the answers

In the context of light interacting with an object, what does refraction refer to?

<p>The bending of light as it slows down when entering a substance (D)</p> Signup and view all the answers

Why does magnification require the bending of light rays?

<p>To bend the rays in order for them to intersect at a focal point (A)</p> Signup and view all the answers

How does a compound microscope compensate for aberration?

<p>By having ocular and objective lenses with different magnifications (C)</p> Signup and view all the answers

How is the detection and resolution of cells under a microscope enhanced?

<p>By making cells adhere to a slide in a fixed position (D)</p> Signup and view all the answers

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