Interactions of Light with Objects
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Questions and Answers

What is resolution in microscopy?

  • The smallest distance by which two objects can be separated and still be distinguished (correct)
  • The ability to determine the presence of an object
  • An increase in the apparent size of an image
  • The area difference between two objects

    • Which type of microscopy is the simplest?

  • Scanning probe microscopy
  • Electron microscopy
  • Fluorescence microscopy
  • Light microscopy (correct)

    • What is detection in terms of observing microbes?

  • Resolution of an image
  • Increase in magnification
  • Differentiation of microbial shapes
  • Determining the presence of an object (correct)

    • What is magnification in microscopy?

    <p>An increase in the apparent size of an image to resolve smaller separations between objects</p> Signup and view all the answers

    What range of sizes do eukaryotic microbes like protozoa, algae, and fungi typically fall within?

    <p>2 µm–20 cm</p> Signup and view all the answers

    What is the main purpose of a simple stain in microbiology?

    <p>Add dark color specifically to cells</p> Signup and view all the answers

    Which technique is used to differentiate between Gram-positive and Gram-negative bacteria?

    <p>Gram Stain</p> Signup and view all the answers

    What happens during fluorescence microscopy in terms of light absorption and emission?

    <p>Specimen absorbs light of defined wavelength and emits light of lower energy</p> Signup and view all the answers

    Which staining technique is known for staining different types of cells to distinguish them from each other?

    <p>Differential Stain</p> Signup and view all the answers

    What is the function of a counterstain in the Gram stain technique?

    <p>To stain Gram-negative bacteria red or pink</p> Signup and view all the answers

    What is the typical size range of prokaryotes?

    <p>0.4–10 µm</p> Signup and view all the answers

    What are common shapes seen in bacteria?

    <p>Bacilli and cocci</p> Signup and view all the answers

    What are the key conditions needed for electromagnetic radiation to resolve an object?

    <p>Contrast between object and medium, smaller wavelength than the object</p> Signup and view all the answers

    What is the wavelength range of visible light?

    <p>~400–750 nm</p> Signup and view all the answers

    How small must the wavelength be compared to the object for electromagnetic radiation to resolve it?

    <p>Smaller than the object</p> Signup and view all the answers

    What does absorption of light by an object mean?

    <p>The object acquires the photon's energy</p> Signup and view all the answers

    In the context of light interacting with an object, what does refraction refer to?

    <p>The bending of light as it slows down when entering a substance</p> Signup and view all the answers

    Why does magnification require the bending of light rays?

    <p>To bend the rays in order for them to intersect at a focal point</p> Signup and view all the answers

    How does a compound microscope compensate for aberration?

    <p>By having ocular and objective lenses with different magnifications</p> Signup and view all the answers

    How is the detection and resolution of cells under a microscope enhanced?

    <p>By making cells adhere to a slide in a fixed position</p> Signup and view all the answers

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