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Instrumental Analysis Lecture 11: Spectroscopy and Separation Techniques

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Instrumental Analysis Lecture 11: Spectroscopy and Separation Techniques

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Questions and Answers

What is electrophoresis?

The migration of charged particles or molecules in a medium under the influence of an applied electric field (correct)

A type of spectroscopy

A type of chromatography

A type of mass spectrometry

What is one of the clinical applications of electrophoresis?

Determining the pH of a solution

Diagnosing Haemoglobinopathies and Haemoglobin A1c (correct)

Purifying water samples

Determining the structure of proteins

What type of molecules can be separated using electrophoresis?

Only proteins and nucleic acids

Only molecules that are not charged

Only small molecules

Charged molecules of any size (correct)

What is the principle of electrophoresis?

The migration of charged particles in a liquid medium under the influence of an electric field Signup and view all the answers

In which type of laboratories is electrophoresis commonly used?

Clinical laboratories Signup and view all the answers

What determines the direction of migration of an ampholyte in an electric field?

The net charge of the molecule Signup and view all the answers

What is the effect of pH on the mobility of an amphoteric molecule?

The mobility is directly proportional to the pH of the solvent Signup and view all the answers

What are the two reservoirs in conventional electrophoresis used for?

To hold the buffer Signup and view all the answers

What is the primary factor that determines the velocity of an ion in an electrical field?

The size and shape of the particle Signup and view all the answers

What is the purpose of using buffers in electrophoresis?

To maintain the pH of the system Signup and view all the answers