Instrumental Analysis Lecture 11: Spectroscopy and Separation Techniques
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Questions and Answers

What is electrophoresis?

  • The migration of charged particles or molecules in a medium under the influence of an applied electric field (correct)
  • A type of spectroscopy
  • A type of chromatography
  • A type of mass spectrometry

What is one of the clinical applications of electrophoresis?

  • Determining the pH of a solution
  • Diagnosing Haemoglobinopathies and Haemoglobin A1c (correct)
  • Purifying water samples
  • Determining the structure of proteins

What type of molecules can be separated using electrophoresis?

  • Only proteins and nucleic acids
  • Only molecules that are not charged
  • Only small molecules
  • Charged molecules of any size (correct)

What is the principle of electrophoresis?

<p>The migration of charged particles in a liquid medium under the influence of an electric field (B)</p> Signup and view all the answers

In which type of laboratories is electrophoresis commonly used?

<p>Clinical laboratories (B)</p> Signup and view all the answers

What determines the direction of migration of an ampholyte in an electric field?

<p>The net charge of the molecule (B)</p> Signup and view all the answers

What is the effect of pH on the mobility of an amphoteric molecule?

<p>The mobility is directly proportional to the pH of the solvent (C)</p> Signup and view all the answers

What are the two reservoirs in conventional electrophoresis used for?

<p>To hold the buffer (A)</p> Signup and view all the answers

What is the primary factor that determines the velocity of an ion in an electrical field?

<p>The size and shape of the particle (C)</p> Signup and view all the answers

What is the purpose of using buffers in electrophoresis?

<p>To maintain the pH of the system (C)</p> Signup and view all the answers

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