18 Questions
How do incubators measure and control CO2 levels?
Via infrared sensors or thermal conductivity
What is required for the thermal conductivity method to give an accurate CO2 reading in incubator chambers?
Fully humidified chambers
How does atmospheric pressure affect the partial pressure of CO2?
Decreases the partial pressure of CO2
What happens to the molar concentration of CO2 as we rise above sea level?
Decreases
Which medium used for human embryo culture had its pH raised to 7.5-7.6 with bicarbonate?
Waymouth's medium
Which culture media was known as BWW IVF pioneers suggested that Earle's medium could be used for the entire process of IVF?
Earle's medium
What is the effect of a drop in temperature on the solubility of CO2 in culture media?
Decreases
What is the relationship between CO2 concentration and pH in culture media?
Inverse relationship
How does the addition of hydrogen ions affect the pH of a solution?
Makes it more acidic
Why is measuring pH preferred over measuring CO2 in incubators?
CO2 levels are not affected by atmospheric pressure
What is the defining property of a buffered solution?
Ability to resist changes in pH
Why does CO2 depress the pH of culture media during equilibration?
CO2 is an acidic gas
Why is Quinn's human tubal fluid (HTF) considered an advancement in embryo culture media?
It has a pH closer to that of human fallopian tube fluid
Which factor contributed to reduced high multiple pregnancy rates in the mid-90s during human embryo culture?
Embryos reaching day 3 stage before transfer
How does a low oxygen environment benefit human embryo culture?
Improves cell numbers and embryo development
What is the preferred energy substrate for human embryo culture?
Pyruvate
Why is there a lack of widespread agreement on simple versus complex media for human embryo culture?
Lack of properly controlled trials to determine the best media
How can embryos be easily cultured in a low oxygen environment?
Using premixed gas that fills the incubator chamber
Test your knowledge on how incubators measure and control CO2 levels, and why pressure fluctuations affect CO2 readings. Learn about the impact of atmospheric pressure on CO2 levels in incubators.
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