Incubator CO2 Level Control Quiz
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Questions and Answers

How do incubators measure and control CO2 levels?

  • Via infrared sensors or thermal conductivity (correct)
  • By analyzing hydrogen levels
  • By measuring oxygen levels
  • Through gravimetric sensors
  • What is required for the thermal conductivity method to give an accurate CO2 reading in incubator chambers?

  • High airflow
  • No humidity control needed
  • Fully humidified chambers (correct)
  • Low humidity levels
  • How does atmospheric pressure affect the partial pressure of CO2?

  • Decreases the partial pressure of CO2 (correct)
  • Has no effect on the partial pressure of CO2
  • Increases the partial pressure of CO2
  • Converts CO2 into oxygen
  • What happens to the molar concentration of CO2 as we rise above sea level?

    <p>Decreases</p> Signup and view all the answers

    Which medium used for human embryo culture had its pH raised to 7.5-7.6 with bicarbonate?

    <p>Waymouth's medium</p> Signup and view all the answers

    Which culture media was known as BWW IVF pioneers suggested that Earle's medium could be used for the entire process of IVF?

    <p>Earle's medium</p> Signup and view all the answers

    What is the effect of a drop in temperature on the solubility of CO2 in culture media?

    <p>Decreases</p> Signup and view all the answers

    What is the relationship between CO2 concentration and pH in culture media?

    <p>Inverse relationship</p> Signup and view all the answers

    How does the addition of hydrogen ions affect the pH of a solution?

    <p>Makes it more acidic</p> Signup and view all the answers

    Why is measuring pH preferred over measuring CO2 in incubators?

    <p>CO2 levels are not affected by atmospheric pressure</p> Signup and view all the answers

    What is the defining property of a buffered solution?

    <p>Ability to resist changes in pH</p> Signup and view all the answers

    Why does CO2 depress the pH of culture media during equilibration?

    <p>CO2 is an acidic gas</p> Signup and view all the answers

    Why is Quinn's human tubal fluid (HTF) considered an advancement in embryo culture media?

    <p>It has a pH closer to that of human fallopian tube fluid</p> Signup and view all the answers

    Which factor contributed to reduced high multiple pregnancy rates in the mid-90s during human embryo culture?

    <p>Embryos reaching day 3 stage before transfer</p> Signup and view all the answers

    How does a low oxygen environment benefit human embryo culture?

    <p>Improves cell numbers and embryo development</p> Signup and view all the answers

    What is the preferred energy substrate for human embryo culture?

    <p>Pyruvate</p> Signup and view all the answers

    Why is there a lack of widespread agreement on simple versus complex media for human embryo culture?

    <p>Lack of properly controlled trials to determine the best media</p> Signup and view all the answers

    How can embryos be easily cultured in a low oxygen environment?

    <p>Using premixed gas that fills the incubator chamber</p> Signup and view all the answers

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