Immunological Methods - Particle Methods
12 Questions
4 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

In immunochemical methods, what happens when an antigen and a specific antibody come together?

  • They undergo a chemical alteration
  • They break down into smaller molecules
  • They create a reversible complex (correct)
  • They form irreversible bonds
  • Which technique involves the creation of a complex through antigen-antibody interaction followed by precipitation?

  • Immunofluorescence
  • Radioimmunoassay
  • ELISA
  • Immunoprecipitation (correct)
  • In immunochemical methods, which technique is primarily used to detect the presence of an antigen or an antibody?

  • Immunoblotting (correct)
  • Rocket electrophoresis
  • Agglutination
  • Two-dimensional crossed immunoelectrophoresis
  • Which immunochemical technique involves the use of fluorescence to detect the location of specific antigens in a sample?

    <p>Immunofluorescence</p> Signup and view all the answers

    Which immunochemical method involves the use of a label attached to either the antigen or antibody for detection?

    <p>Immunofluorescence</p> Signup and view all the answers

    In which immunochemical method is a precipitate visually detected for analysis?

    <p>Immunoelectrophoresis</p> Signup and view all the answers

    Which method uses a molecule attached to either the antigen or antibody for detection?

    <p>Immunoassays</p> Signup and view all the answers

    Which immunochemical method commonly employs immunoglobulins (IgA, IgG, IgM) to bind specifically to antigens or haptens?

    <p>Immunoblotting</p> Signup and view all the answers

    What type of detector operates on high voltage to measure ionising radiation by producing an avalanche of electrons?

    <p>Geiger-Müller (GM) counter detector</p> Signup and view all the answers

    In the described method, which type of radiation can the Geiger-Müller (GM) counter detect?

    <p>Both alpha and beta radiation</p> Signup and view all the answers

    What creates a small ionisation current in the ionising chamber?

    <p>Ion pairs migrating due to voltage between electrodes</p> Signup and view all the answers

    Which method is suitable for a wide range of energies and preferred for high levels of gamma radiation detection?

    <p>Ionising chamber using lower voltage</p> Signup and view all the answers

    Study Notes

    Immunochemical Methods

    • When an antigen and a specific antibody come together, they form a complex through antigen-antibody interaction, which can lead to precipitation.
    • The technique that involves the creation of a complex through antigen-antibody interaction followed by precipitation is known as precipitation reaction.
    • The primary technique used to detect the presence of an antigen or an antibody is immunoassay.
    • Fluorescence immunoassay involves the use of fluorescence to detect the location of specific antigens in a sample.
    • The technique that involves the use of a label attached to either the antigen or antibody for detection is known as labelled immunoassay.
    • In precipitin reaction, a precipitate is visually detected for analysis.
    • The method that uses a molecule attached to either the antigen or antibody for detection is called labelled immunoassay.
    • The method that commonly employs immunoglobulins (IgA, IgG, IgM) to bind specifically to antigens or haptens is called immunoassay.

    Radiation Detection

    • The Geiger-Müller (GM) counter is a type of detector that operates on high voltage to measure ionizing radiation by producing an avalanche of electrons.
    • The Geiger-Müller (GM) counter can detect beta and gamma radiation.
    • Ionizing radiation creates a small ionization current in the ionizing chamber.
    • The Geiger-Müller (GM) counter is suitable for a wide range of energies and is preferred for high levels of gamma radiation detection.

    Studying That Suits You

    Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

    Quiz Team

    Description

    Learn about basic principles of antigen and antibody interaction along with techniques like immunoprecipitation in gel, passive gel immunodiffusion, and immunoelectrophoresis. This quiz is tailored for internal teaching at the Department of Chemical Pathology, NHLS- Wits Medical School.

    More Like This

    Microbiology Laboratory Techniques Quiz
    17 questions
    Immunological Assay Methods
    10 questions
    Immunological Methods Quiz
    10 questions

    Immunological Methods Quiz

    SophisticatedTulsa2777 avatar
    SophisticatedTulsa2777
    Use Quizgecko on...
    Browser
    Browser