Podcast
Questions and Answers
Which of the following best describes what a transform does in the context of image analysis?
Which of the following best describes what a transform does in the context of image analysis?
- It always converts a color image to grayscale.
- It directly enhances the resolution of an image.
- It removes all noise from the image.
- It maps image data from one mathematical space to another. (correct)
Orthogonal basis images are preferred in image transforms because they introduce redundant information.
Orthogonal basis images are preferred in image transforms because they introduce redundant information.
False (B)
What key parameter does the Modulation Transfer Function (MTF) quantify in imaging systems?
What key parameter does the Modulation Transfer Function (MTF) quantify in imaging systems?
contrast
The Nyquist criterion dictates the minimum ______ frequency required to avoid losing information during digitization.
The Nyquist criterion dictates the minimum ______ frequency required to avoid losing information during digitization.
Match the following imaging modalities with their primary strengths:
Match the following imaging modalities with their primary strengths:
What is the purpose of applying digital pulses to the gates in CCD (Charge-Coupled Device) image sensors?
What is the purpose of applying digital pulses to the gates in CCD (Charge-Coupled Device) image sensors?
Photomultiplier Tubes (PMTs) store electric changes, making them less ideal for extremely fast events compared to CCDs.
Photomultiplier Tubes (PMTs) store electric changes, making them less ideal for extremely fast events compared to CCDs.
What type of distribution do detected photons follow, which is important for understanding noise characteristics?
What type of distribution do detected photons follow, which is important for understanding noise characteristics?
In fluorescence, the emission of light occurs at a ______ energy (larger wavelength) compared to the absorbed light.
In fluorescence, the emission of light occurs at a ______ energy (larger wavelength) compared to the absorbed light.
Which of the following is a key advantage of using 2-photon excitation microscopy?
Which of the following is a key advantage of using 2-photon excitation microscopy?
X-rays are considered non-ionizing radiation, making them safe for all types of clinical imaging.
X-rays are considered non-ionizing radiation, making them safe for all types of clinical imaging.
What is the appropriate probe wavelength length requirement for achieving adequate resolution?
What is the appropriate probe wavelength length requirement for achieving adequate resolution?
What is the order of relative X-ray absorption in the human body (from least to most absorption)?
What is the order of relative X-ray absorption in the human body (from least to most absorption)?
The goal during image reconstruction of the inner structure is to recover it from the ______.
The goal during image reconstruction of the inner structure is to recover it from the ______.
Radiation exposure creates a unique cancer risk readily distinguished from cancer risk due to other environmental factors.
Radiation exposure creates a unique cancer risk readily distinguished from cancer risk due to other environmental factors.
Which type of image compression is generally recommended when it is especially important to preserve all of the original image data?
Which type of image compression is generally recommended when it is especially important to preserve all of the original image data?
According to the FDA's suggestion, what should be done before analysis of images to reduce file archiving demands?
According to the FDA's suggestion, what should be done before analysis of images to reduce file archiving demands?
Match the following terms with their definitions related to Fourier Transforms:
Match the following terms with their definitions related to Fourier Transforms:
What are the two different methods used for visualizing 3D image data?
What are the two different methods used for visualizing 3D image data?
Stereotactic intervention utilizes a ______ dimensional coordinate system to accurately locate targets in the body
Stereotactic intervention utilizes a ______ dimensional coordinate system to accurately locate targets in the body
In computer graphics, the procedure involves linking points to display mathematical curves and areas?
In computer graphics, the procedure involves linking points to display mathematical curves and areas?
What type of information is attributed to points, edges, and areas when forming polygons in polygonal modeling?
What type of information is attributed to points, edges, and areas when forming polygons in polygonal modeling?
One limitation of Polygonal Modeling is that it has difficulty rendering ______ lines and patches.
One limitation of Polygonal Modeling is that it has difficulty rendering ______ lines and patches.
Match the definition with the correct term:
Match the definition with the correct term:
Surface triangulation involves creating a topological skeleton of an object?
Surface triangulation involves creating a topological skeleton of an object?
What is the term for the process of assigning colors to different sections of an image based on depth?
What is the term for the process of assigning colors to different sections of an image based on depth?
A ______ is the three-dimensional equivalent of a pixel.
A ______ is the three-dimensional equivalent of a pixel.
Which of the following features are associated with volume rendition?
Which of the following features are associated with volume rendition?
Image Order Rendering uses orthogonal projection to create images?
Image Order Rendering uses orthogonal projection to create images?
What are the two types of intensities that are handled in Volume Rendering?
What are the two types of intensities that are handled in Volume Rendering?
Blending controls transparency or ______ by color, the color
is a RGB-vector.
Blending controls transparency or ______ by color, the color
is a RGB-vector.
Which parameter is NOT used in the additional control of brightness?
Which parameter is NOT used in the additional control of brightness?
3-D image processing is same as slice-by-slice image filtering.
3-D image processing is same as slice-by-slice image filtering.
The cubes of voxels of what size are analyzed with the Marching Cube?
The cubes of voxels of what size are analyzed with the Marching Cube?
For proper implant fitting into 3D Image Volume, ______ geometry is used.
For proper implant fitting into 3D Image Volume, ______ geometry is used.
Which of the following is a surface rendering technique?
Which of the following is a surface rendering technique?
Optical Tomography includes an increase, but not a reduction of blurr.
Optical Tomography includes an increase, but not a reduction of blurr.
In the diagram, what is the name of the step following topology in the overall workflow options?
In the diagram, what is the name of the step following topology in the overall workflow options?
______ is required for quantitive assesment in volume rendering.
______ is required for quantitive assesment in volume rendering.
Which of the following is a benefit of surface rendering over volume rendering?
Which of the following is a benefit of surface rendering over volume rendering?
In Object Order Rendering, the process goes from the back to the front.
In Object Order Rendering, the process goes from the back to the front.
Name the type of projection that Object Order Rendering focuses on.
Name the type of projection that Object Order Rendering focuses on.
In stereoscopic CRT display systems, there is ______ multiplexed and time parallel.
In stereoscopic CRT display systems, there is ______ multiplexed and time parallel.
What is a key advantage of Volume Rendering over Surface Rendering?
What is a key advantage of Volume Rendering over Surface Rendering?
What is increased with optical tomography?
What is increased with optical tomography?
In the surface rendering workflow, ______ geometry is used.
In the surface rendering workflow, ______ geometry is used.
Which parameter is used with Image Order Rendering?
Which parameter is used with Image Order Rendering?
Which of the following is a characteristic of surface rendering?
Which of the following is a characteristic of surface rendering?
What is surface triangulation?
What is surface triangulation?
What is one advantage of surface rendering?
What is one advantage of surface rendering?
What is one benefit of Object Ordering Rendering?
What is one benefit of Object Ordering Rendering?
What is one use of Surface Rendering?
What is one use of Surface Rendering?
What is color depth coding?
What is color depth coding?
Which of the following techniques is used in 3D Visualization process?
Which of the following techniques is used in 3D Visualization process?
What are the benifits to Non-invasive Serial Sectioning?
What are the benifits to Non-invasive Serial Sectioning?
What is a limitation of Surface Rendering?
What is a limitation of Surface Rendering?
What is a key point about Virtual Reality in 3-D Visualization?
What is a key point about Virtual Reality in 3-D Visualization?
Which of the following is a limitation of polygonal modeling in 3D imaging?
Which of the following is a limitation of polygonal modeling in 3D imaging?
In volume rendering, 'Image Order Rendering' projects voxels orthogonally onto the screen.
In volume rendering, 'Image Order Rendering' projects voxels orthogonally onto the screen.
What is the primary function of blending in Volume Rendering regarding voxel intensities?
What is the primary function of blending in Volume Rendering regarding voxel intensities?
In 3D imaging, a pixel in a 2D image is analogous to a ______ in a 3D volume.
In 3D imaging, a pixel in a 2D image is analogous to a ______ in a 3D volume.
Match the following steps to create a visualization:
Match the following steps to create a visualization:
What is considered the basic cellular unit of the brain?
What is considered the basic cellular unit of the brain?
Extracellular recordings can only record the activity of a single neuron at a time.
Extracellular recordings can only record the activity of a single neuron at a time.
Invasive recordings that use sharp or patch electrodes are known as ______ recordings.
Invasive recordings that use sharp or patch electrodes are known as ______ recordings.
Which of the following materials is NOT typically used for electrodes in extracellular recordings?
Which of the following materials is NOT typically used for electrodes in extracellular recordings?
Intracellular recordings can measure subthreshold responses in neurons.
Intracellular recordings can measure subthreshold responses in neurons.
What term is used to describe the emission of light by an organism as the result of a chemical reaction?
What term is used to describe the emission of light by an organism as the result of a chemical reaction?
What happens when a molecule undergoes fluorescence?
What happens when a molecule undergoes fluorescence?
In fluorescence, the emitted light has a smaller wavelength than the absorbed light.
In fluorescence, the emitted light has a smaller wavelength than the absorbed light.
The process where a molecule absorbs a high-energy photon, placing it in a higher energy state, is a key step in ______.
The process where a molecule absorbs a high-energy photon, placing it in a higher energy state, is a key step in ______.
Voltage-sensitive dyes are used to:
Voltage-sensitive dyes are used to:
Voltage-sensitive dyes need to be genetically encoded to be used in neurons.
Voltage-sensitive dyes need to be genetically encoded to be used in neurons.
In the context of voltage-sensitive dyes, what does PeT stand for?
In the context of voltage-sensitive dyes, what does PeT stand for?
A major challenge with using dyes for imaging is the need to ______ to the specific neurons of interest.
A major challenge with using dyes for imaging is the need to ______ to the specific neurons of interest.
What is the primary advantage of using genetically encoded indicators over synthetic dyes?
What is the primary advantage of using genetically encoded indicators over synthetic dyes?
The use of GFP in cells allows for the cell itself to ______ the protein, which eliminates the need to inject synthetic dyes.
The use of GFP in cells allows for the cell itself to ______ the protein, which eliminates the need to inject synthetic dyes.
Which of the following is a limitation of using fluorescent proteins alone for imaging neural activity?
Which of the following is a limitation of using fluorescent proteins alone for imaging neural activity?
Name one type of sensor, other than calcium sensors, that can be combined with fluorescent proteins to image neural activity.
Name one type of sensor, other than calcium sensors, that can be combined with fluorescent proteins to image neural activity.
To image neural activity, fluorescent proteins are combined with proteins that change conformation in response to that activity, such as calcium sensors or ______ sensors.
To image neural activity, fluorescent proteins are combined with proteins that change conformation in response to that activity, such as calcium sensors or ______ sensors.
What is the purpose of combining fluorescent proteins with other proteins to image neural activity?
What is the purpose of combining fluorescent proteins with other proteins to image neural activity?
Fluorescent proteins can act as indicators for neuronal activity all by themselves.
Fluorescent proteins can act as indicators for neuronal activity all by themselves.
What does GECI stand for in the context of neural imaging?
What does GECI stand for in the context of neural imaging?
GCaMP is an example of a ______ indicator used in neural imaging.
GCaMP is an example of a ______ indicator used in neural imaging.
Which of the following is a well-known type of genetically encoded calcium indicator (GECI)?
Which of the following is a well-known type of genetically encoded calcium indicator (GECI)?
In two-photon microscopy, two photons combine to have the same effect as multiple lower energy photons.
In two-photon microscopy, two photons combine to have the same effect as multiple lower energy photons.
Name the technique that permits deeper tissue penetration and minimizes photobleaching by exciting fluorescent proteins only at the focal point where two photons interact.
Name the technique that permits deeper tissue penetration and minimizes photobleaching by exciting fluorescent proteins only at the focal point where two photons interact.
In two-photon microscopy, excitation of fluorescent proteins occurs only where two photons ______, reducing photobleaching.
In two-photon microscopy, excitation of fluorescent proteins occurs only where two photons ______, reducing photobleaching.
Which of the following is a key advantage of two-photon microscopy over single-photon microscopy?
Which of the following is a key advantage of two-photon microscopy over single-photon microscopy?
Two-photon microscopy results in more photobleaching than one-photon microscopy.
Two-photon microscopy results in more photobleaching than one-photon microscopy.
What is the proposed function of the Drosophila central complex?
What is the proposed function of the Drosophila central complex?
The Drosophila central complex is thought to be involved in visual processing.
The Drosophila central complex is thought to be involved in visual processing.
Light sheet microscopy allows you to image neural activity in an organism.
Light sheet microscopy allows you to image neural activity in an organism.
In light sheet microscopy, are samples illuminated from only one side?
In light sheet microscopy, are samples illuminated from only one side?
In microscopy, light ______ is a method in which samples are illuminated with just a 'sheet' of light.
In microscopy, light ______ is a method in which samples are illuminated with just a 'sheet' of light.
Light sheet microscopy is useful to image neural activity for what reason?
Light sheet microscopy is useful to image neural activity for what reason?
What is the primary advantage of using genetically encoded indicators, like GFP, for imaging neural activity, compared to synthetic dyes?
What is the primary advantage of using genetically encoded indicators, like GFP, for imaging neural activity, compared to synthetic dyes?
Extracellular recordings utilize sharp electrodes to record subthreshold responses from neurons.
Extracellular recordings utilize sharp electrodes to record subthreshold responses from neurons.
What property of a molecule is key to fluorescence?
What property of a molecule is key to fluorescence?
Two-photon microscopy reduces photobleaching because excitation occurs only where two ______ interact.
Two-photon microscopy reduces photobleaching because excitation occurs only where two ______ interact.
Which type of microscopy is particularly useful for imaging deeper into tissue with reduced photobleaching?
Which type of microscopy is particularly useful for imaging deeper into tissue with reduced photobleaching?
Match each voltage indicator with its correct description:
Match each voltage indicator with its correct description:
Which of the following is a limitation of imaging with dyes?
Which of the following is a limitation of imaging with dyes?
Fluorescent proteins can directly act as indicators of neural activity without needing additional components.
Fluorescent proteins can directly act as indicators of neural activity without needing additional components.
In the context of fluorescence, what happens when a molecule absorbs a high-energy photon?
In the context of fluorescence, what happens when a molecule absorbs a high-energy photon?
What is the basic cellular unit of the brain?
What is the basic cellular unit of the brain?
Which of the following is NOT typically considered an area of use for clinical imaging methods?
Which of the following is NOT typically considered an area of use for clinical imaging methods?
In X-ray imaging, increasing the radiation dose always leads to a decrease in the signal-to-noise ratio (SNR).
In X-ray imaging, increasing the radiation dose always leads to a decrease in the signal-to-noise ratio (SNR).
State the absorption law used in X-ray imaging.
State the absorption law used in X-ray imaging.
In X-ray imaging, contrast is based on differential ________ of radiation by different tissues.
In X-ray imaging, contrast is based on differential ________ of radiation by different tissues.
Match the following tissue types with their relative absorption of X-rays, from lowest to highest:
Match the following tissue types with their relative absorption of X-rays, from lowest to highest:
What is a primary disadvantage of projection X-ray imaging?
What is a primary disadvantage of projection X-ray imaging?
Perfusion imaging techniques can only be CT-based.
Perfusion imaging techniques can only be CT-based.
Name two parameters measured by sophisticated post-processing software in perfusion imaging.
Name two parameters measured by sophisticated post-processing software in perfusion imaging.
The development of _________ in imaging allowed for true 3D imaging.
The development of _________ in imaging allowed for true 3D imaging.
Which of the following describes a key requirement for a probe used in medical imaging?
Which of the following describes a key requirement for a probe used in medical imaging?
Ultrasound imaging uses electromagnetic (EM) energy.
Ultrasound imaging uses electromagnetic (EM) energy.
Give one advantage and one disadvantage of ultrasound imaging.
Give one advantage and one disadvantage of ultrasound imaging.
In ultrasound imaging, resolution is proportional to _________.
In ultrasound imaging, resolution is proportional to _________.
Which of the following best describes how X-ray imaging generates an image?
Which of the following best describes how X-ray imaging generates an image?
Magnetic Resonance (MR) imaging is generally considered unsafe due to the use of ionizing radiation.
Magnetic Resonance (MR) imaging is generally considered unsafe due to the use of ionizing radiation.
What physical property does ultrasound measure to create images?
What physical property does ultrasound measure to create images?
In Magnetic Resonance imaging, the signal, M(x,y,z), is proportional to _________.
In Magnetic Resonance imaging, the signal, M(x,y,z), is proportional to _________.
Which imaging modality is generally the first choice for Neuro/Head assessment of bone structures?
Which imaging modality is generally the first choice for Neuro/Head assessment of bone structures?
Ultrasound is generally considered an excellent imaging modality for visualizing structures within the abdomen, regardless of air or gas interference.
Ultrasound is generally considered an excellent imaging modality for visualizing structures within the abdomen, regardless of air or gas interference.
Which modality could be merged with CT for extensive use in the heart?
Which modality could be merged with CT for extensive use in the heart?
For cardiovascular imaging, X-ray imaging is excellent when combined with ________ contrast.
For cardiovascular imaging, X-ray imaging is excellent when combined with ________ contrast.
Which imaging modality provides functional information on perfusion in the skeletal system?
Which imaging modality provides functional information on perfusion in the skeletal system?
MRI is considered not useful for imaging the cardiovascular system.
MRI is considered not useful for imaging the cardiovascular system.
For skeletal/muscular imaging, which modality is strong for viewing the skeletal system?
For skeletal/muscular imaging, which modality is strong for viewing the skeletal system?
Which of the following lists the modalities in order of capital cost, from lowest to highest?
Which of the following lists the modalities in order of capital cost, from lowest to highest?
Which of the following is a primary reason for using image compression?
Which of the following is a primary reason for using image compression?
All images benefit equally from compression techniques, regardless of their content.
All images benefit equally from compression techniques, regardless of their content.
What type of redundancy is exploited when compressing neighboring pixels that are not independent but correlated?
What type of redundancy is exploited when compressing neighboring pixels that are not independent but correlated?
The difference between information and data is that data includes ______ data.
The difference between information and data is that data includes ______ data.
In the context of image compression, what does 'quantization' refer to?
In the context of image compression, what does 'quantization' refer to?
Increasing the number of quantization levels always results in better image compression.
Increasing the number of quantization levels always results in better image compression.
Name one factor, besides storage space, that makes image compression necessary.
Name one factor, besides storage space, that makes image compression necessary.
The uncompressed bit rate of digital cinema data can exceed 1 ______ per second.
The uncompressed bit rate of digital cinema data can exceed 1 ______ per second.
In image compression, 'spatial redundancy' refers to:
In image compression, 'spatial redundancy' refers to:
Spatial redundancy is about similarities that exist across multiple frames in a video sequence.
Spatial redundancy is about similarities that exist across multiple frames in a video sequence.
What term is used to describe the bits needed to represent an image?
What term is used to describe the bits needed to represent an image?
Image compression exploits the fact that all images are not equally ______.
Image compression exploits the fact that all images are not equally ______.
Which type of redundancy considers how the human eye perceives visual information when compressing images?
Which type of redundancy considers how the human eye perceives visual information when compressing images?
Psychovisual redundancy relies on similarities that exist across frames in a video sequence.
Psychovisual redundancy relies on similarities that exist across frames in a video sequence.
What equation represents the composition of data in terms of information and redundancy?
What equation represents the composition of data in terms of information and redundancy?
In the equation Bit Rate = NQF
, N
represents the number of ______.
In the equation Bit Rate = NQF
, N
represents the number of ______.
What does decreasing 'Q' (Quantization bits/pixel) in the equation Bit Rate = NQF
create?
What does decreasing 'Q' (Quantization bits/pixel) in the equation Bit Rate = NQF
create?
Decreasing frame rate will increase resolution
Decreasing frame rate will increase resolution
What is a primary goal in image compression, besides reducing file size?
What is a primary goal in image compression, besides reducing file size?
Modern image processing is typically performed in the ______ domain.
Modern image processing is typically performed in the ______ domain.
Which of these is true of lossless compression?
Which of these is true of lossless compression?
Lossy image compression techniques guarantee perfect image data reconstruction upon decompression.
Lossy image compression techniques guarantee perfect image data reconstruction upon decompression.
What type of coding is used by lossless compression?
What type of coding is used by lossless compression?
JPEG is an example of a ______ image compression method.
JPEG is an example of a ______ image compression method.
Which of these file formats is typically associated with lossless compression?
Which of these file formats is typically associated with lossless compression?
JPEG files are best suited for logos and textual graphics.
JPEG files are best suited for logos and textual graphics.
What term describes the artifacts that can appear in an image from excessive compression?
What term describes the artifacts that can appear in an image from excessive compression?
In JPEG encoding, the 2D forward ______ is applied to preprocess the image.
In JPEG encoding, the 2D forward ______ is applied to preprocess the image.
What comes after image preprocessing in JPEG encoding steps?
What comes after image preprocessing in JPEG encoding steps?
After Quantization, apply 2D forward DCT.
After Quantization, apply 2D forward DCT.
In the JPEG Block Diagram, what immediately precedes the Entropy Encoder?
In the JPEG Block Diagram, what immediately precedes the Entropy Encoder?
In JPEG compression, color transformation is ______.
In JPEG compression, color transformation is ______.
What is the main purpose of applying the Forward DCT (Discrete Cosine Transform) in image compression?
What is the main purpose of applying the Forward DCT (Discrete Cosine Transform) in image compression?
DFT is preferred over DCT for better approximations.
DFT is preferred over DCT for better approximations.
The intensity values in each color plane often change how?
The intensity values in each color plane often change how?
______ is when the resulting image is 'close' to the original image.
______ is when the resulting image is 'close' to the original image.
According to the provided text, what should be done if in doubt about whether to use lossy or lossless compression?
According to the provided text, what should be done if in doubt about whether to use lossy or lossless compression?
Image compression is a new field with lots of room for improvement.
Image compression is a new field with lots of room for improvement.
What type of redundancy describes predictions along motion trajectories?
What type of redundancy describes predictions along motion trajectories?
_____DPCM is used to remove temporal redundancy first.
_____DPCM is used to remove temporal redundancy first.
Which of the following accurately describes a primary reason for employing image compression techniques?
Which of the following accurately describes a primary reason for employing image compression techniques?
Spatial redundancy in images refers to the independence of neighboring pixels, meaning the value of one pixel does not correlate with the values of adjacent pixels.
Spatial redundancy in images refers to the independence of neighboring pixels, meaning the value of one pixel does not correlate with the values of adjacent pixels.
Briefly explain how image compression exploits the characteristics of images to reduce file size.
Briefly explain how image compression exploits the characteristics of images to reduce file size.
In the context of image compression, the two main categories are ______ compression, which can reproduce the original data exactly, and ______ compression, which sacrifices some data to achieve higher compression ratios.
In the context of image compression, the two main categories are ______ compression, which can reproduce the original data exactly, and ______ compression, which sacrifices some data to achieve higher compression ratios.
Match the following image compression concepts with their descriptions:
Match the following image compression concepts with their descriptions:
Flashcards
What is a Transform?
What is a Transform?
A mathematical mapping process; provides info on rate of gray level change within an image (spatial frequency).
What is Point Spread Function (PSF)?
What is Point Spread Function (PSF)?
Describes how a single point of light is distributed or spread out by the imaging system.
What is Modulation Transfer Function (MTF)?
What is Modulation Transfer Function (MTF)?
Quantifies how well an imaging system preserves contrast at different spatial frequencies; relates to detail transmission.
What is Noise in imaging?
What is Noise in imaging?
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What is Resolution?
What is Resolution?
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What is the Nyquist Criterion?
What is the Nyquist Criterion?
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What is image sampling?
What is image sampling?
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What are CCD Image Sensors?
What are CCD Image Sensors?
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What are Photomultiplier Tubes (PMTs)?
What are Photomultiplier Tubes (PMTs)?
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What are Computerized Tomography (CT) methods?
What are Computerized Tomography (CT) methods?
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What is Ultrasound (US)?
What is Ultrasound (US)?
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What is Nuclear (PET/SPECT) imaging?
What is Nuclear (PET/SPECT) imaging?
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What are Fluorescence techniques?
What are Fluorescence techniques?
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What is Image Compression?
What is Image Compression?
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What is Lossless Compression?
What is Lossless Compression?
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What is Lossy Compression?
What is Lossy Compression?
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What does Fourier Transform do?
What does Fourier Transform do?
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What are Reconstructions from Projections?
What are Reconstructions from Projections?
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Compare Surface vs Volume rendering.
Compare Surface vs Volume rendering.
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What are Stereotactic interventions?
What are Stereotactic interventions?
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Computer Graphics
Computer Graphics
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Polygonal Modeling
Polygonal Modeling
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Bézier-Polynomial
Bézier-Polynomial
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Bézier-Curves
Bézier-Curves
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Topological Interpretation
Topological Interpretation
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Surface Triangulation
Surface Triangulation
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Marching Cubes
Marching Cubes
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Optical Tomography
Optical Tomography
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Non-invasive serial sectioning
Non-invasive serial sectioning
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Color depth coding
Color depth coding
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Voxel
Voxel
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Volume Rendering
Volume Rendering
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Artificial lightning
Artificial lightning
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Image Order Rendering
Image Order Rendering
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Object Order Rendering
Object Order Rendering
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Blending of intensities
Blending of intensities
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Segmentation
Segmentation
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Region of Interest (ROI)
Region of Interest (ROI)
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Embedded Geometry
Embedded Geometry
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Topology
Topology
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Neuron
Neuron
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Invasive Recordings
Invasive Recordings
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Intracellular Recording
Intracellular Recording
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Extracellular Recording
Extracellular Recording
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Fluorescence
Fluorescence
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Bioluminescence
Bioluminescence
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Dye Delivery
Dye Delivery
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Aequorea victoria
Aequorea victoria
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Green Fluorescent Protein (GFP)
Green Fluorescent Protein (GFP)
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Fluorescent Proteins
Fluorescent Proteins
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Voltage Sensitive Dyes
Voltage Sensitive Dyes
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Calcium/Transmembrane voltage sensors
Calcium/Transmembrane voltage sensors
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Genetically Encoded Calcium Indicators (GECIs)
Genetically Encoded Calcium Indicators (GECIs)
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cpGFP voltage sensors
cpGFP voltage sensors
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Light Sheet Microscopy
Light Sheet Microscopy
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Two-Photon Microscopy
Two-Photon Microscopy
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Diagnostic Imaging
Diagnostic Imaging
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Screening Imaging
Screening Imaging
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Therapy Control Imaging
Therapy Control Imaging
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Intervention Imaging
Intervention Imaging
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Basic Biomedical Research Imaging
Basic Biomedical Research Imaging
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Radiation Dosis
Radiation Dosis
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Costs and Time (Imaging)
Costs and Time (Imaging)
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Medical Imaging Limitations
Medical Imaging Limitations
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(Physical) Resolution
(Physical) Resolution
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(Time) Resolution
(Time) Resolution
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Image Matrix
Image Matrix
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Imaging Measurement
Imaging Measurement
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Signal-to-Noise Ratio (SNR)
Signal-to-Noise Ratio (SNR)
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X-ray Divergence
X-ray Divergence
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X-ray Penetration
X-ray Penetration
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X-ray Absorption Law
X-ray Absorption Law
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X-ray Contrast
X-ray Contrast
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X-Ray Imaging
X-Ray Imaging
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Contrast Agent
Contrast Agent
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Perfusion Imaging
Perfusion Imaging
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3D Imaging
3D Imaging
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Necessary Probe Properties
Necessary Probe Properties
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X-ray Modality
X-ray Modality
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CT modality
CT modality
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Ultrasound modality
Ultrasound modality
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What is Bit Rate Reduction?
What is Bit Rate Reduction?
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How does image compression work?
How does image compression work?
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What are energy gaps?
What are energy gaps?
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What is spatial redundancy?
What is spatial redundancy?
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What is temporal redundancy?
What is temporal redundancy?
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Psychovisual redundancy
Psychovisual redundancy
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What is Bandwidth Compression?
What is Bandwidth Compression?
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What is the main goal of image compression?
What is the main goal of image compression?
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What is the digital domain?
What is the digital domain?
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Reducing N,Q,F
Reducing N,Q,F
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What is BMP (Bitmap)?
What is BMP (Bitmap)?
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Graphic Interchange Format (GIF)
Graphic Interchange Format (GIF)
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What is JPEG?
What is JPEG?
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What are 'Blocking Artifacts'?
What are 'Blocking Artifacts'?
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What are the JPEG encoding steps?
What are the JPEG encoding steps?
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What is color transformation?
What is color transformation?
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What is Forward DCT?
What is Forward DCT?
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Why DCT not DFT?
Why DCT not DFT?
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What is Reconstruction Error?
What is Reconstruction Error?
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Temporal DPCM
Temporal DPCM
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Motion estimation influence?
Motion estimation influence?
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What is Motion Compensated Prediction?
What is Motion Compensated Prediction?
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Study Notes
Image Compression
- Image compression reduces the number of bits needed to represent an image or its information.
- It exploits the fact that all images are not equally likely and energy gaps in the signal.
Why Compression is Needed
- Requirements may exceed the anticipated increase of storage space and bandwidth.
- Data storage and transmission, video conferencing, and printers benefit from compression.
- The bitrate of uncompressed digital cinema data exceeds 1 Gbps.
- Video streams require compression.
Why Compression Works
- Spatial redundancy exists because neighboring pixels are not independent, but correlated.
- Temporal redundancy.
Psychovisual Redundancy
- Quantization is an example.
- Adds a small pseudo-random number to each pixel before quantization.
Information vs. Data
- Data = Information + Redundant Data.
Image Model
- The picture size is one unit wide by one unit high.
- M = number of samples.
- D = spacing between samples = 1/√M
- Correlation between adjacent samples = e^D = e^(1/√M)
Compression and Image Content
- Portrait (fills 1/2 frame) has a correlation distance^-1 of 6.3.
- Typical (moderate detail) has a correlation distance^-1 of 16.7.
- 100 people has a correlation distance^-1 of 50.
- 2000 people has a correlation distance^-1 of 150.
Interframe and Intraframe Processing
- Interframe processing involves point-to-point predictive encoding and line-to-line encoding.
- Intraframe Processing
Bit Rate
- Bit Rate = NQF.
- N = Number of pixels.
- Q = Quantization bits/pixel.
- F = Frame rate.
- Compression Ratio = 10 LOG [Channel Bit Rate / NQF].
Reducing Parameters
- Reducing N results in reduced resolution.
- Reducing Q results in contouring (artifacts).
- Reducing F results in image blur.
- More sophisticated approaches are needed.
Goal of Image Compression
- Storing image data as efficiently as possible.
- Maximizing image quality and minimizing storage space and processing resources.
- Trade-offs are necessary because you can't have the best of both.
Digital vs. Analog
- Modern image processing is done in the digital domain.
- Analog source images get converted to digital format before processing.
Lossless Compression
- Stored image data can reproduce the original image exactly.
- Lossless compression typically takes more storage space.
- Lossless compression only uses entropy coding or none at all.
- Examples: BMP, TIFF, GIF.
Lossy Compression
- Stored image data can reproduce something that looks "close" to the original image.
- Lossy Compression uses both quantization and entropy coding.
- Lossy Compression involves transform into frequency or other domain.
- Examples: JPEG, JPEG-2000.
BMP (Bitmap)
- Uses 3 bytes per pixel: one each for R, G, and B.
- Can represent up to 2^24 = 16.7 million colors.
- No entropy coding.
- File size in bytes = 3 * length * height, which can be very large.
- Can use fewer than 8 bits per color, but need to store the color palette.
- Works well with ZIP, RAR, etc.
GIF (Graphics Interchange Format)
- Can use up to 256 colors from 24-bit RGB color space.
- If a source image contains more than 256 colors, reprocessing is needed to reduce the image to fewer colors.
- Suitable for simpler images such as logos and textual graphics, not photographs.
- Uses LZW lossless data compression.
JPEG (Joint Photographic Experts Group)
- Dominant image format today.
- Typical file size is about 10% of BMP (can vary depending on quality settings).
- Unlike GIF, JPEG is suitable for photographs, not logos and textual graphics.
JPEG Encoding Steps
- Preprocess image.
- Apply 2D forward DCT.
- Quantize DCT coefficients.
- Apply RLE, then entropy encoding.
Color Transformation
- (Optional) Down-sample chrominance components to compress without loss of quality in color space as an option
- Examples include: YUV 4:2:2 or 4:1:1
- 640 x 480 RGB to YUV 4:1:1 example consists of: Y is 640x480, U is 160x120, and V is 160x120.
Forward DCT
- Convert from spatial to frequency domain.
- Convert the intensity function into a weighted sum of periodic basis (cosine) functions.
- Identify bands of spectral information that can be thrown away without loss of quality.
- Intensity values in each color plane often change slowly.
Why DCT Not DFT
- DCT is similar to DFT, but can provide a better approximation with fewer coefficients.
- The coefficients of DCT are real-valued instead of complex-valued in DFT.
Understanding DCT
- In R^3, we can write (5, 2, 9) as the sum of a set of basis vectors: [(1,0,0), (0,1,0), (0,0,1)].
- (5,2,9) = 5*(1,0,0) + 2*(0,1,0) + 9*(0,0,1).
- DCT is the same process in the function domain.
DCT Basis Functions
- Decompose the intensity function into a...
Reconstruction Error
- The resulting image is "close" to the original image.
- Closeness measures use MSE (Mean Squared Error) and PSNR (Peak Signal to Noise Ratio).
- Desire low MSE and high PSNR.
Image Compression in Regulated Environments
- Previously employed image acquisition, analysis, compression.
- Image Compression utilizes, Image acquisition, compression, analysis.
Image Formats in Regulated Studies
- JPEG compression does not alter the accuracy or reliability to reproduce individual data point measurements, except at the highest compression level.
- If acquired with TIFF and saved as JPEG, there may be discordance.
- If the original and features extracted from a JPEG-compressed file, high confidence in the reproducibility of individual measures.
Quantitative Analysis and Compression
- High-quality classifications can be obtained with JPEG compression ratios up to 10:1 or higher.
- High compression tends to eliminate much of the pixel-to-pixel detail.
- Training on compressed imagery could raise the training site accuracy, but did not raise the percentage of pixels matching the original classification.
- The maximum-likelihood classifier degraded faster than other classifiers due to specific assumptions about class statistics.
- Data tends to become uncorrelated with high compression, ruining its statistics.
What is MPEG
- "Motion Picture Expert Group" was established in 1990 to create standards for the delivery of audio and video.
- MPEG-1 targets VHS quality on a CD-ROM(320x240 + CD audio @ 1.5 Mbit/sec).
MPEG (Interframe Coding)
- Temporal DPCM removes temporal redundancy first.
- Motion compensated error is coded with DCT+Run length Huffman entropy coding.
- Spatial Compression(Image compression)
- Termporal Compression.(Sequence compression)
- MPEG-2 has a ratio of 100:1
- MPEG-4 has a ratio of 200:1
MPEG Temporal Redundancy
- Prediction along the motion trajectories (motion compensation prediction).
Motion Estimation
- The accuracy of motion estimation has a big influence on coding efficiency.
- Motion estimation is time-consuming.
- Fast algorithms are needed.
MPEG-1 Frame Types
- I frame is an intra-coded frame.
- P frame is an inter-coded or predicted frame.
Conclusion
- Image compression can be done lossy or lossless.
- Use lossless if in doubt.
- The degree of noise will impact the amount of compression.
- Image compression is nearly mature, but there is always room for improvement.
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