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HPLC Technology

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Questions and Answers

Which of the following is NOT a type of high performance liquid chromatography column?

Normal-phase

Size-exclusion

Solid-phase (correct)

Reverse-phase

What does the stationary phase do in high performance liquid chromatography?

Carries the sample through the column

Moves with the mobile phase

Detects the sample components

Separates the sample components based on interactions (correct)

Which detector is commonly used in high performance liquid chromatography for compounds that absorb UV or visible light?

Flame ionization detector

Refractive index detector (correct)

Diode array detector

Mass spectrometer

What is the primary purpose of gel electrophoresis in molecular biology?

To separate DNA fragments by size Signup and view all the answers

Which of the following gels is most commonly used for DNA separation?

Agarose Signup and view all the answers

In high performance liquid chromatography, which phase is responsible for carrying the sample through the column?

Mobile phase Signup and view all the answers

Study Notes

High Performance Liquid Chromatography (HPLC)

Solid-phase is not a type of HPLC column.
HPLC columns can be classified into three types: Reverse-phase, Normal-phase, and Size-exclusion.
The stationary phase in HPLC separates sample components based on interactions.

HPLC Detectors

The Refractive Index Detector is commonly used in HPLC for compounds that absorb UV or visible light.
Other types of detectors used in HPLC include the Diode Array Detector, Mass Spectrometer, and Flame Ionization Detector.

Gel Electrophoresis

The primary purpose of gel electrophoresis in molecular biology is to separate DNA fragments by size.
Agarose gel is the most commonly used gel for DNA separation.
Other applications of gel electrophoresis do not include amplifying DNA sequences, sequencing DNA, or synthesizing RNA.

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Description

A quiz on high performance liquid chromatography, covering types of columns and stationary phase functions.