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Questions and Answers
Which of the following is a defining characteristic of S. cerevisiae strains?
Which of the following is a defining characteristic of S. cerevisiae strains?
- The ability to exist as both haploid and diploid cells (correct)
- The ability to undergo sporulation to form four-spored asci
- The ability to grow and store indefinitely as haploid cells
- The ability to manipulate for gene expression studies
What must be present in yeast media?
What must be present in yeast media?
- A carbon and nitrogen source (correct)
- Agar supplements
- Amino acids
- All of the above
Why should amino acids not be added before autoclaving SD medium?
Why should amino acids not be added before autoclaving SD medium?
- They can interfere with the autoclaving process
- They can negatively affect the growth of yeast cells (correct)
- They can cause the medium to become too acidic
- They can cause the medium to become too basic
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Study Notes
- Saccharomyces cerevisiae is a fungus used in fermentation and molecular biology.
- It can exist as haploid or diploid cells.
- Haploid cells can be grown and stored indefinitely.
- Diploid cells can undergo sporulation to form four-spored asci.
- Yeast strains are defined by ploidy status and genetic markers.
- S. cerevisiae is easy to manipulate for gene expression studies.
- Yeast media must contain a carbon and nitrogen source, and may contain supplements and agar.
- Nutritional markers are commonly used for selection of transformants and mating diploids.
- Amino acids should not be added before autoclaving SD medium.
- Re-melting SD agar media can present problems with setting hard.
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