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Questions and Answers
Which of the following is a defining characteristic of S. cerevisiae strains?
Which of the following is a defining characteristic of S. cerevisiae strains?
- The ability to exist as both haploid and diploid cells (correct)
- The ability to undergo sporulation to form four-spored asci
- The ability to grow and store indefinitely as haploid cells
- The ability to manipulate for gene expression studies
What must be present in yeast media?
What must be present in yeast media?
- A carbon and nitrogen source (correct)
- Agar supplements
- Amino acids
- All of the above
Why should amino acids not be added before autoclaving SD medium?
Why should amino acids not be added before autoclaving SD medium?
- They can interfere with the autoclaving process
- They can negatively affect the growth of yeast cells (correct)
- They can cause the medium to become too acidic
- They can cause the medium to become too basic
Study Notes
- Saccharomyces cerevisiae is a fungus used in fermentation and molecular biology.
- It can exist as haploid or diploid cells.
- Haploid cells can be grown and stored indefinitely.
- Diploid cells can undergo sporulation to form four-spored asci.
- Yeast strains are defined by ploidy status and genetic markers.
- S. cerevisiae is easy to manipulate for gene expression studies.
- Yeast media must contain a carbon and nitrogen source, and may contain supplements and agar.
- Nutritional markers are commonly used for selection of transformants and mating diploids.
- Amino acids should not be added before autoclaving SD medium.
- Re-melting SD agar media can present problems with setting hard.
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Description
Test your knowledge of Saccharomyces cerevisiae, the versatile fungus used in fermentation and molecular biology. Learn about its ploidy status, growth conditions, genetic markers, and more. This quiz will challenge your understanding of yeast media, nutritional markers, and gene expression studies. Don't miss the opportunity to improve your knowledge of this fascinating organism!
