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Questions and Answers
What do capital letters represent in DNA?
What do capital letters represent in DNA?
- Restriction enzymes
- Bases (correct)
- Single-stranded extensions
- Complementary bases
What do blunt ends lack that prevents them from circularizing spontaneously?
What do blunt ends lack that prevents them from circularizing spontaneously?
- Complementary bases
- Staggered breaks
- Restriction enzymes
- Single-stranded extensions (correct)
What did Werner Arber, Hamilton Smith, and Daniel Nathans win the Nobel Prize for?
What did Werner Arber, Hamilton Smith, and Daniel Nathans win the Nobel Prize for?
- Discovering DNA
- Characterizing hundreds of restriction enzymes
- Recognizing the usefulness of restriction enzymes (correct)
- Breaking DNA at specific recognition sequences
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Study Notes
- Capital letters represent bases and ' indicates the complementary base.
- Restriction enzymes break DNA at specific recognition sequences.
- Breaks can be staggered or blunt.
- Staggered breaks form cohesive ends with single-stranded extensions.
- Blunt ends cannot circularize spontaneously.
- Each restriction enzyme generates a unique set of fragments.
- Hundreds of restriction enzymes have been characterized.
- Most attack short palindromic target sequences.
- Some recognize other types of specific sequences.
- Werner Arber, Hamilton Smith, and Daniel Nathans were awarded the 1978 Nobel Prize for recognizing the usefulness of restriction enzymes.
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